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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/09/1995 |
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Data da última atualização: |
27/09/1995 |
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Autoria: |
KITTS, W. D.; UNDERKOFLER, L. A. |
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Título: |
Digestion by rumen microorganisms: hydrolytic produts of cellulose and the cellulolytic enzymes. |
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Ano de publicação: |
1954 |
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Fonte/Imprenta: |
Journal Agricultural and Food Chemistry, v.2, n.12, p.639-645, June,1954. |
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Idioma: |
Inglês |
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Conteúdo: |
The chemistry of cellulose digestion by rumen microorganisms was investigated with spe-
cial reference to identification of the carbohydrates formed and the cellulolytic enzymes.
In order to identify the carbohydrate intermediates, a number or organic and inorganic
compounds were tested for their ability to arrest fermentation by rumen microorganisms at
the reducing sugar stage. Among those found effective were toluene, thymol, and sodium fluoride. Inhibited cellulose-digesting cultures were prepared and the media analyzed at
hourly intervals for the carbohydrate intemediates formed. Filter paper chromatography re-
vealed the principal intermediate compound of cellulose degradation by rumen organisms
to be glucose. The cellulolytic enzymes of rumen microorganisms are not present as such
in rumen fluid, but are associated with the bacterial cells, as centrifuged and filtered rumen
fluid failed to digest cellulose. Cell-free enzyme extracts of mixed rumen culture and of an
ísolated rumen organims were prepared which degraded cellulosic substrates. An optimum
temperature of 40 C. and an optimum pH of 5.5 were found for the maximum activity of the cell-free preparations. The cell-free preparations are stable at low temperature, but are par-
tially inactivated when allowed to stand at room temperature for 144 hours. |
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Palavras-Chave: |
Hydrolytic products; Microorganismo; Ruminant. |
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Thesagro: |
Digestão; Enzima; Ruminante. |
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Thesaurus Nal: |
cellulose; digestion; rumen microorganisms. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02017naa a2200241 a 4500
001 1786360
005 1995-09-27
008 1954 bl --- 0-- u #d
100 1 $aKITTS, W. D.
245 $aDigestion by rumen microorganisms$bhydrolytic produts of cellulose and the cellulolytic enzymes.
260 $c1954
520 $aThe chemistry of cellulose digestion by rumen microorganisms was investigated with spe- cial reference to identification of the carbohydrates formed and the cellulolytic enzymes. In order to identify the carbohydrate intermediates, a number or organic and inorganic compounds were tested for their ability to arrest fermentation by rumen microorganisms at the reducing sugar stage. Among those found effective were toluene, thymol, and sodium fluoride. Inhibited cellulose-digesting cultures were prepared and the media analyzed at hourly intervals for the carbohydrate intemediates formed. Filter paper chromatography re- vealed the principal intermediate compound of cellulose degradation by rumen organisms to be glucose. The cellulolytic enzymes of rumen microorganisms are not present as such in rumen fluid, but are associated with the bacterial cells, as centrifuged and filtered rumen fluid failed to digest cellulose. Cell-free enzyme extracts of mixed rumen culture and of an ísolated rumen organims were prepared which degraded cellulosic substrates. An optimum temperature of 40 C. and an optimum pH of 5.5 were found for the maximum activity of the cell-free preparations. The cell-free preparations are stable at low temperature, but are par- tially inactivated when allowed to stand at room temperature for 144 hours.
650 $acellulose
650 $adigestion
650 $arumen microorganisms
650 $aDigestão
650 $aEnzima
650 $aRuminante
653 $aHydrolytic products
653 $aMicroorganismo
653 $aRuminant
700 1 $aUNDERKOFLER, L. A.
773 $tJournal Agricultural and Food Chemistry$gv.2, n.12, p.639-645, June,1954.
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Registro original: |
Embrapa Pantanal (CPAP) |
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