| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
31/03/2009 |
|
Data da última atualização: |
30/06/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LEÓN, M. G.; BECERRA, C. H.; FREITAS-ASTÚA, J.; SALAROLI, R. B.; KITAJIMA, E. W. |
|
Afiliação: |
M. G. León, CORPOICA; C. H. Becerra, ICA; Juliana Freitas-Ástua, CNPMF; R. B. Salaroli, ESALQ; Elliot Watanabe Kitajima, ESALQ. |
|
Título: |
Natural infection of Swinglea glutinosa by the Citrus leprosis virus cytoplasmic type (CiLV-C) in Colombia. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
Plant Disease, v.92, n.9, p.1364, 2008. |
|
Idioma: |
Inglês |
|
Notas: |
DOI: 10.1094/PDIS-92-9-1364C |
|
Conteúdo: |
Swinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5'GATACGGGACGCATAACA-3'/Rev. 5'-TTCTGGCTCAACATCTGG-3') that specifically amplify a region within the CiLV-C putative methyltransferase gene and this yielded a single fragment of the expected 402 bp (3). Analysis of the consensus sequence derived from 20 RT-PCR products (GenBank Accession No. EU689106) showed 96% nucleotide and 92% amino acid sequence identity to the sequence of a Brazilian CiLV-C isolate (GenBank Accession Nos. DQ352194.1 and YP_654565.1), respectively. Recently, published work described mite transmission of CiLV-C to some nonrutaceous plants (1), but to our knowledge, this is the first report of a nonCitrus rutaceous plant naturally infected by CiLV-C. Mites found in citrus orchards and previously identified as Brevipalpus phoenicis (Geijskes) (2), which are likely the most important vector of CiLV-C in citrus in Colombia, were observed feeding on healthy and symptomatic S. glutinosa, indicating that S. glutinosa is a host for B. phoenicis. Because the use of S. glutinosa as a living fence or hedge is a common practice in Colombia, CiLV-C-infected S. glutinosa plants may play a role in the epidemiology of leprosis in commercial citrus by serving as an inoculum source for this lethal virus. MenosSwinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5... Mostrar Tudo |
|
Thesagro: |
Ácaro; Doença de Planta; Leprose; Vírus. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03395naa a2200229 a 4500
001 1655641
005 2023-06-30
008 2008 bl uuuu u00u1 u #d
100 1 $aLEÓN, M. G.
245 $aNatural infection of Swinglea glutinosa by the Citrus leprosis virus cytoplasmic type (CiLV-C) in Colombia.$h[electronic resource]
260 $c2008
500 $aDOI: 10.1094/PDIS-92-9-1364C
520 $aSwinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5'GATACGGGACGCATAACA-3'/Rev. 5'-TTCTGGCTCAACATCTGG-3') that specifically amplify a region within the CiLV-C putative methyltransferase gene and this yielded a single fragment of the expected 402 bp (3). Analysis of the consensus sequence derived from 20 RT-PCR products (GenBank Accession No. EU689106) showed 96% nucleotide and 92% amino acid sequence identity to the sequence of a Brazilian CiLV-C isolate (GenBank Accession Nos. DQ352194.1 and YP_654565.1), respectively. Recently, published work described mite transmission of CiLV-C to some nonrutaceous plants (1), but to our knowledge, this is the first report of a nonCitrus rutaceous plant naturally infected by CiLV-C. Mites found in citrus orchards and previously identified as Brevipalpus phoenicis (Geijskes) (2), which are likely the most important vector of CiLV-C in citrus in Colombia, were observed feeding on healthy and symptomatic S. glutinosa, indicating that S. glutinosa is a host for B. phoenicis. Because the use of S. glutinosa as a living fence or hedge is a common practice in Colombia, CiLV-C-infected S. glutinosa plants may play a role in the epidemiology of leprosis in commercial citrus by serving as an inoculum source for this lethal virus.
650 $aÁcaro
650 $aDoença de Planta
650 $aLeprose
650 $aVírus
700 1 $aBECERRA, C. H.
700 1 $aFREITAS-ASTÚA, J.
700 1 $aSALAROLI, R. B.
700 1 $aKITAJIMA, E. W.
773 $tPlant Disease$gv.92, n.9, p.1364, 2008.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Meio Ambiente. |
|
Data corrente: |
13/01/2010 |
|
Data da última atualização: |
25/02/2011 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
C - 0 |
|
Autoria: |
BERTIN, P. R. B.; VACARI, I.; SIMÃO, V. P. M.; VISOLI, M. C.; LEITE, F. C. L. |
|
Afiliação: |
PATRÍCIA ROCHA BELLO BERTIN, SCT; ISAQUE VACARI, CNPTIA; VICTOR PAULO MARQUES SIMÃO, CNPMA; MARCOS CEZAR VISOLI, CNPTIA; FERNANDO CÉSAR LIMA LEITE, SCT. |
|
Título: |
An Open Access Approach to scientific information management at the brazilian agricultural research corporation. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Scholarly and Research Communication, v. 1, 1, p. 1-12, 2010. |
|
Idioma: |
Inglês |
|
Conteúdo: |
This article presents the experience of the Brazilian Agricultural Research Corporation (Embrapa) - a large state-owned company that plays an important global role in research, development, and innovation for tropical agriculture - in the planning and implementation of Open Access to scientific information in the context of a developing country. The aim of this initiative is to provide the necessary mechanisms to capture, store, organize, preserve, retrieve, and widely disseminate the scientific information produced by Embrapa and by agricultural research communities. This report concludes with a discussion of the obstacles encountered and the organizational features, cultural considerations, and political matters that facilitate open access implementation at Embrapa. |
|
Palavras-Chave: |
Acesso livre; Artigo científico; Embrapa; Open Access Approach; Repositório institucional; Scientific information management. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/17246/1/1-130-2-PB.Open.pdf
|
|
Marc: |
LEADER 01541naa a2200241 a 4500
001 1580209
005 2011-02-25
008 2010 bl uuuu u00u1 u #d
100 1 $aBERTIN, P. R. B.
245 $aAn Open Access Approach to scientific information management at the brazilian agricultural research corporation.$h[electronic resource]
260 $c2010
520 $aThis article presents the experience of the Brazilian Agricultural Research Corporation (Embrapa) - a large state-owned company that plays an important global role in research, development, and innovation for tropical agriculture - in the planning and implementation of Open Access to scientific information in the context of a developing country. The aim of this initiative is to provide the necessary mechanisms to capture, store, organize, preserve, retrieve, and widely disseminate the scientific information produced by Embrapa and by agricultural research communities. This report concludes with a discussion of the obstacles encountered and the organizational features, cultural considerations, and political matters that facilitate open access implementation at Embrapa.
653 $aAcesso livre
653 $aArtigo científico
653 $aEmbrapa
653 $aOpen Access Approach
653 $aRepositório institucional
653 $aScientific information management
700 1 $aVACARI, I.
700 1 $aSIMÃO, V. P. M.
700 1 $aVISOLI, M. C.
700 1 $aLEITE, F. C. L.
773 $tScholarly and Research Communication$gv. 1, 1, p. 1-12, 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
