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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
02/12/2005 |
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Data da última atualização: |
21/08/2009 |
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Autoria: |
GASPAR, J. O.; BELINTANI, P.; ALMEIDA, A. M. R.; KITAJIMA, E. W. |
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Título: |
A primer pair allows amplification of part of the 3'-terminal of carlaviruses genome. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Virus Reviews & Research, Rio de Janeiro, v. 10, p. 140, Nov. 2005. Supplement, ref. P-191. |
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Idioma: |
Inglês |
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Notas: |
Edição dos Resumos da XVI National Meeting of Virology, nov. 2005. |
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Conteúdo: |
The genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP gene showed the highest identify (78,3% nt and 93,5% aa) with the isolate H of Cowpea mild mottle virus (AF024628). Our designed primer have the advantage that about 930 nt from the 3' terminus, comprising part of the CP gene, the 11K gene and the terminal untranslated region can be amplified for sequencing, allowing the detection of carlaviruses and identification of unknown viruses as mebers of this virus genus. MenosThe genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP ge... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02495naa a2200169 a 4500
001 1468611
005 2009-08-21
008 2005 bl --- 0-- u #d
100 1 $aGASPAR, J. O.
245 $aA primer pair allows amplification of part of the 3'-terminal of carlaviruses genome.
260 $c2005
500 $aEdição dos Resumos da XVI National Meeting of Virology, nov. 2005.
520 $aThe genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP gene showed the highest identify (78,3% nt and 93,5% aa) with the isolate H of Cowpea mild mottle virus (AF024628). Our designed primer have the advantage that about 930 nt from the 3' terminus, comprising part of the CP gene, the 11K gene and the terminal untranslated region can be amplified for sequencing, allowing the detection of carlaviruses and identification of unknown viruses as mebers of this virus genus.
700 1 $aBELINTANI, P.
700 1 $aALMEIDA, A. M. R.
700 1 $aKITAJIMA, E. W.
773 $tVirus Reviews & Research, Rio de Janeiro$gv. 10, p. 140, Nov. 2005. Supplement, ref. P-191.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/05/2013 |
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Data da última atualização: |
24/06/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
GODOY, R. C. B. de; WASZCZYNSKJ, N.; SANTANA, F. A.; SILVA, S. de O. e; SOUZA NETO, M. A.; OLIVEIRA, L. A. de; LEDO, C. A. da S. |
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Afiliação: |
ROSSANA CATIE BUENO DE GODOY, CNPF; NINA WASZCZYNSKJ, UFPR; FERNANDA ALVES SANTANA, Universidade Federal do Recôncavo Baiano; SEBASTIÃO DE OLIVEIRA E SILVA, Pesquisador aposentado da Embrapa Mandioca e Fruticultura Tropical; MANUEL A. SOUZA NETO, Ex pesquisador da Embrapa Agroindústria Tropical; LUCIANA ALVES DE OLIVEIRA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Banana cultivars with resistance to black leaf streak and potential for jam processing. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Acta Horticulturae, n. 986, p. 309-317, 2013. |
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Idioma: |
Inglês |
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Notas: |
Trabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses. Eds.: I. Van den Bergh et al. |
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Conteúdo: |
Dessert banana cultivars used in the agro-industry belong mainly to the Cavendish subgroup (AAA), highly susceptible to black leaf streak (BLS). The objective of this study was to compare physicochemical and sensory data of banana jams using fruits of BLS-resistant genotypes with that of jams made from the traditional cultivar ?Grande Naine?. Variables evaluated were pH, titratable acidity TTA, soluble solids, total solids, yield, total sugars, reducing sugars and nonreducing sugars, humidity, water activity, firmness, adhesiveness and color. The multisample difference test was used for sensory analysis. There were no differences among the treatments for total soluble solids, total solids, humidity, brightness and yellowness. The total tritratable acidity and reducing sugars were superior in jams obtained from resistant genotypes. The non-reducing sugars in the jams produced from ?Bucaneiro?, ?Calipso?, ?Thap Maeo?, ?FHIA-18? and ?FHIA-02? were inferior compared to ?Grande Naine?, and the firmness of the jams from ?Thap Maeo?, ?Caipira? and ?FHIA-18? was superior to that of ?Grande Naine?. The highest adhesiveness was found in jams from ?Caipira? and the highest redness in jams from ?Thap Maeo?. The sugars were the variables responsible for the greatest diversity. In the cluster analysis, jam from ?Grande Naine? was close to the ?Caipira?, ?Ambrosia?, ?Calipso? and ?Bucaneiro? jams. Jam from ?Thap Maeo? was the most distant from ?Grande Naine?. The judges did not notice any significant differences in flavor for the jams produced with ?Bucaneiro?, ?Calipso?, ?Thap Maeo?, ?FHIA-02? and ?FHIA-18? in comparison to the ?Grande Naine? control. ?FHIA-18? had a slightly better performance than ?Grande Naine?. These results indicate that some banana genotypes with BLS resistance can be recommended for the production of jams, yielding similar or superior quality as ?Grande Naine?. MenosDessert banana cultivars used in the agro-industry belong mainly to the Cavendish subgroup (AAA), highly susceptible to black leaf streak (BLS). The objective of this study was to compare physicochemical and sensory data of banana jams using fruits of BLS-resistant genotypes with that of jams made from the traditional cultivar ?Grande Naine?. Variables evaluated were pH, titratable acidity TTA, soluble solids, total solids, yield, total sugars, reducing sugars and nonreducing sugars, humidity, water activity, firmness, adhesiveness and color. The multisample difference test was used for sensory analysis. There were no differences among the treatments for total soluble solids, total solids, humidity, brightness and yellowness. The total tritratable acidity and reducing sugars were superior in jams obtained from resistant genotypes. The non-reducing sugars in the jams produced from ?Bucaneiro?, ?Calipso?, ?Thap Maeo?, ?FHIA-18? and ?FHIA-02? were inferior compared to ?Grande Naine?, and the firmness of the jams from ?Thap Maeo?, ?Caipira? and ?FHIA-18? was superior to that of ?Grande Naine?. The highest adhesiveness was found in jams from ?Caipira? and the highest redness in jams from ?Thap Maeo?. The sugars were the variables responsible for the greatest diversity. In the cluster analysis, jam from ?Grande Naine? was close to the ?Caipira?, ?Ambrosia?, ?Calipso? and ?Bucaneiro? jams. Jam from ?Thap Maeo? was the most distant from ?Grande Naine?. The judges did not notice any ... Mostrar Tudo |
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Palavras-Chave: |
Agroindústria; Agroindustry; Composição; Composition; Dessert; Sobremesa. |
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Thesagro: |
Alimento preparado; Banana; Doce; Indústria agrícola. |
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Thesaurus NAL: |
Bananas; Desserts; Food preparation; Multivariate analysis. |
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Categoria do assunto: |
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Marc: |
LEADER 03045naa a2200373 a 4500
001 1958981
005 2013-06-24
008 2013 bl uuuu u00u1 u #d
100 1 $aGODOY, R. C. B. de
245 $aBanana cultivars with resistance to black leaf streak and potential for jam processing.
260 $c2013
500 $aTrabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses. Eds.: I. Van den Bergh et al.
520 $aDessert banana cultivars used in the agro-industry belong mainly to the Cavendish subgroup (AAA), highly susceptible to black leaf streak (BLS). The objective of this study was to compare physicochemical and sensory data of banana jams using fruits of BLS-resistant genotypes with that of jams made from the traditional cultivar ?Grande Naine?. Variables evaluated were pH, titratable acidity TTA, soluble solids, total solids, yield, total sugars, reducing sugars and nonreducing sugars, humidity, water activity, firmness, adhesiveness and color. The multisample difference test was used for sensory analysis. There were no differences among the treatments for total soluble solids, total solids, humidity, brightness and yellowness. The total tritratable acidity and reducing sugars were superior in jams obtained from resistant genotypes. The non-reducing sugars in the jams produced from ?Bucaneiro?, ?Calipso?, ?Thap Maeo?, ?FHIA-18? and ?FHIA-02? were inferior compared to ?Grande Naine?, and the firmness of the jams from ?Thap Maeo?, ?Caipira? and ?FHIA-18? was superior to that of ?Grande Naine?. The highest adhesiveness was found in jams from ?Caipira? and the highest redness in jams from ?Thap Maeo?. The sugars were the variables responsible for the greatest diversity. In the cluster analysis, jam from ?Grande Naine? was close to the ?Caipira?, ?Ambrosia?, ?Calipso? and ?Bucaneiro? jams. Jam from ?Thap Maeo? was the most distant from ?Grande Naine?. The judges did not notice any significant differences in flavor for the jams produced with ?Bucaneiro?, ?Calipso?, ?Thap Maeo?, ?FHIA-02? and ?FHIA-18? in comparison to the ?Grande Naine? control. ?FHIA-18? had a slightly better performance than ?Grande Naine?. These results indicate that some banana genotypes with BLS resistance can be recommended for the production of jams, yielding similar or superior quality as ?Grande Naine?.
650 $aBananas
650 $aDesserts
650 $aFood preparation
650 $aMultivariate analysis
650 $aAlimento preparado
650 $aBanana
650 $aDoce
650 $aIndústria agrícola
653 $aAgroindústria
653 $aAgroindustry
653 $aComposição
653 $aComposition
653 $aDessert
653 $aSobremesa
700 1 $aWASZCZYNSKJ, N.
700 1 $aSANTANA, F. A.
700 1 $aSILVA, S. de O. e
700 1 $aSOUZA NETO, M. A.
700 1 $aOLIVEIRA, L. A. de
700 1 $aLEDO, C. A. da S.
773 $tActa Horticulturae$gn. 986, p. 309-317, 2013.
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