Data/hora: 23/06/2024 01:03:51

Biblioteca(s): | Embrapa Florestas.
Data corrente: | 04/01/1993
Data da última atualização: | 04/01/1993
Autoria: | TSUKADA, M.; KUSANO, T.; KITAGAWA, Y.
Título: | Introduction of foreign genes into tomato protoplasts by electroporation.
Ano de publicação: | 1989
Fonte/Imprenta: | Plant Cell Physiology, Kyoto, v.30, n.4, p.599-604, 1989.
Idioma: | Inglês
Conteúdo: | Optimum transfer of a plasmid harbouring the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves was achieved by electroporation at 666 or 7000 V/cm (single discharge) using a 47 5F capacitor in the presence of 10-100 5g DNA. The CAT gene was strongly expressed when under the control of the CaMV 35S RNA promoter. CAT activity was detected in cells 10 days after electroporation.
Palavras-Chave: | Eletroporacao; Promoter; Protoplastos; Technique; Tomato; Vegetable.
Thesagro: | Biotecnologia; Engenharia Genética.
Thesaurus Nal: | biotechnology; chloramphenicol acetyltransferase; electroporation; genetic transformation.
Categoria do assunto: | --
Registro original: | Embrapa Florestas (CNPF)
Exemplares: | CNPF - (1110-1) - ADC - SP - -- - SP0178 -  - SP0178 - DIS