| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
|
Data corrente: |
01/11/1995 |
|
Data da última atualização: |
01/11/1995 |
|
Autoria: |
CHOI, P. S.; SOH, W. Y.; KIM, Y. S.; YOO, O. J.; LIU, J. R. |
|
Título: |
Genetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens. |
|
Ano de publicação: |
1994 |
|
Fonte/Imprenta: |
Plant Cell Reports, v.13, n.6, p.344-348, 1994. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Adventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai; cvs. Sweet Gem and Gold Medal] cultured on Murashige and Skoog's (MS) medium with 1 mgl-1 6-benzyladenine (BA). Light (16-h photoperiod, about 7 Wm-2 cool-white fluorescent lamps) was essential for shoot formation. To obtain transformed plants, cotyledonary explants of "Sweet Gem" were cocultured with Agrobacterium tumefaciens LBA4404, a disarmed strain harboring a binary vector pBI121 carrying the CaMV 35S promoter-belta-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker, for 48h on MS medium with 1 mgl-1 BA and 200 uM beta-hydroxyacetosyringone. After 48h of culture, explants were transferred to medium with 1 mgl-1 BA, 250 mgl-1 carbenicillin, and 100 mgl-1 kanamycin and cultured in the light. Adventitious shoots formed on the explants after 4 weeks of culture. When subjected to GUS histochemical assay, young leaves obtained from the shoots showed a positive response at a frequency of up to 16%. Preculturing cotyledonary explants on MS medium with 1 mgl-1 BA for 5d enhanced the competence of the cells to be transformed by Agrobacterium Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants. The transformed plants were grown to maturity. |
|
Palavras-Chave: |
Melhoramento genetico; Watermelon. |
|
Thesagro: |
Agrobacterium Tumefaciens; Melancia. |
|
Thesaurus Nal: |
genetic transformation. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02095naa a2200229 a 4500
001 1075311
005 1995-11-01
008 1994 bl --- 0-- u #d
100 1 $aCHOI, P. S.
245 $aGenetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens.
260 $c1994
520 $aAdventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai; cvs. Sweet Gem and Gold Medal] cultured on Murashige and Skoog's (MS) medium with 1 mgl-1 6-benzyladenine (BA). Light (16-h photoperiod, about 7 Wm-2 cool-white fluorescent lamps) was essential for shoot formation. To obtain transformed plants, cotyledonary explants of "Sweet Gem" were cocultured with Agrobacterium tumefaciens LBA4404, a disarmed strain harboring a binary vector pBI121 carrying the CaMV 35S promoter-belta-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker, for 48h on MS medium with 1 mgl-1 BA and 200 uM beta-hydroxyacetosyringone. After 48h of culture, explants were transferred to medium with 1 mgl-1 BA, 250 mgl-1 carbenicillin, and 100 mgl-1 kanamycin and cultured in the light. Adventitious shoots formed on the explants after 4 weeks of culture. When subjected to GUS histochemical assay, young leaves obtained from the shoots showed a positive response at a frequency of up to 16%. Preculturing cotyledonary explants on MS medium with 1 mgl-1 BA for 5d enhanced the competence of the cells to be transformed by Agrobacterium Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants. The transformed plants were grown to maturity.
650 $agenetic transformation
650 $aAgrobacterium Tumefaciens
650 $aMelancia
653 $aMelhoramento genetico
653 $aWatermelon
700 1 $aSOH, W. Y.
700 1 $aKIM, Y. S.
700 1 $aYOO, O. J.
700 1 $aLIU, J. R.
773 $tPlant Cell Reports$gv.13, n.6, p.344-348, 1994.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 5 | |
| 3. |  | LADISCH, M. R.; XIMENES, E.; FARINAS, C. S.; KIM, Y.; KO, J. K.; KREKE, T. Mechanisms of Lignin Derived Inhibition in Hydrolysis of Pretreated Biomass at Low Enzyme Loadings. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 38., 2016, Baltimore. Papers. [S. l.: s. n.], 2016. P. 12-1.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
|    |
| 4. | | HONG, J.; KIM, D.; CHO, K.; SA, S.; CHOI, S.; KIM, Y.; PARK, J.; SCHMIDT, G. S.; DAVIS, M. E.; CHUNG, H. Effects of genetic variants for the swine FABP3, HMGA1, MC4R, IGF2, and FABP4 genes on fatty acid composition. Meat Science, v. 110, p. 46-51, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 5. | | HENGGE, N.; KIM, D.; MOSIER, N. S.; KIM, Y.; XIMENES, E.; LADISCH, M. R.; CUNHA, F.; BADINO, A. C.; FARINAS, C. S. Liquefaction of lignocellulose. In: AICHE ANNUAL MEETING, 2016. Abstracts... [S. l.: s. n.], 2016. n. 474787.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
| |
| Registros recuperados : 5 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
