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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/11/2021 |
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Data da última atualização: |
04/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FALEIRO, F. G.; KIM, J. Y.; PARIKH, A.; ALTPETER, F. |
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Afiliação: |
FABIO GELAPE FALEIRO, CPAC. |
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Título: |
Generation of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Tropical Plant Biology, Sep. 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Napier grass (Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the production of biofuel. Genetic engineering and genome editing have tremendous potential for crop improvement and require the establishment of a transformation protocol. In this study, transgenic Napier grass was generated by introducing a minimal cassette (MC) encoding the antibiotic resistance gene nptII with biolistic gene transfer into embryogenic callus. Paromomycin was superior to geneticin as a selective agent in the selection of transgenic calli. Two high-biomass producing Napier grass accessions were successfully transformed. Southern blot analysis revealed simple transgene integration patterns with 33% of the regenerated transgenic lines having a single insert of the minimal transgene expression cassette. NPTII-ELISA confirmed transgene expression in all transgenic lines. The results of this study will enable the acceleration of Napier grass improvement by genetic transformation or genome editing. |
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Thesagro: |
Capim Napier; Organismo Transgênico. |
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Thesaurus Nal: |
Genetic engineering; Transgenic plants. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01698naa a2200205 a 4500
001 2136186
005 2023-04-04
008 2021 bl uuuu u00u1 u #d
100 1 $aFALEIRO, F. G.
245 $aGeneration of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette.$h[electronic resource]
260 $c2021
520 $aNapier grass (Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the production of biofuel. Genetic engineering and genome editing have tremendous potential for crop improvement and require the establishment of a transformation protocol. In this study, transgenic Napier grass was generated by introducing a minimal cassette (MC) encoding the antibiotic resistance gene nptII with biolistic gene transfer into embryogenic callus. Paromomycin was superior to geneticin as a selective agent in the selection of transgenic calli. Two high-biomass producing Napier grass accessions were successfully transformed. Southern blot analysis revealed simple transgene integration patterns with 33% of the regenerated transgenic lines having a single insert of the minimal transgene expression cassette. NPTII-ELISA confirmed transgene expression in all transgenic lines. The results of this study will enable the acceleration of Napier grass improvement by genetic transformation or genome editing.
650 $aGenetic engineering
650 $aTransgenic plants
650 $aCapim Napier
650 $aOrganismo Transgênico
700 1 $aKIM, J. Y.
700 1 $aPARIKH, A.
700 1 $aALTPETER, F.
773 $tTropical Plant Biology, Sep. 2021.
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