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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
22/11/2019 |
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Data da última atualização: |
10/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIANA, C.; SERENO, M. J.; BERSOT, L. dos S.; KICH, J. D.; NERO, L. A. |
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Afiliação: |
CIBELI VIANA, UFV; MALLU JAGNOW SERENO, UFPR; LUCIANO DOS SANTOS BERSOT, UFPR; JALUSA DEON KICH, CNPSA; LUIS AUGUSTO NERO, UFV. |
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Título: |
Comparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Foodborne Pathogens and Disease, v. 17, n. 3, p. 194-201, 2020. |
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DOI: |
10.1089/fpd.2019.2712 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering. MenosAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the except... Mostrar Tudo |
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Thesagro: |
Bacteriologia; Cadeia Produtiva; Carne; Elisa; Enzima; Salmonella; Suíno; Técnica Imunoenzimática. |
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Thesaurus Nal: |
Bacteriology; Disease surveillance; Enzyme-linked immunosorbent assay; Isolation techniques; Pork. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02918naa a2200337 a 4500
001 2114901
005 2020-03-10
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1089/fpd.2019.2712$2DOI
100 1 $aVIANA, C.
245 $aComparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain.$h[electronic resource]
260 $c2020
520 $aAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering.
650 $aBacteriology
650 $aDisease surveillance
650 $aEnzyme-linked immunosorbent assay
650 $aIsolation techniques
650 $aPork
650 $aBacteriologia
650 $aCadeia Produtiva
650 $aCarne
650 $aElisa
650 $aEnzima
650 $aSalmonella
650 $aSuíno
650 $aTécnica Imunoenzimática
700 1 $aSERENO, M. J.
700 1 $aBERSOT, L. dos S.
700 1 $aKICH, J. D.
700 1 $aNERO, L. A.
773 $tFoodborne Pathogens and Disease$gv. 17, n. 3, p. 194-201, 2020.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
20/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, J. F. de; CAVALCANTI, J. J. V.; FARIAS, F. J. C.; RAMOS, J. P. C.; QUEIROZ, D. R.; SANTOS, R. C. dos. |
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Afiliação: |
Jarbas Florentino de Carvalho, Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; FRANCISCO JOSE CORREIA FARIAS, CNPA; Jean Pierre Cordeiro Ramos, Universidade Federal da Paraíba - UFPB/Centro de Ciências Agrárias; Damião Raniere Queiroz, Universidade Federal Rural de Pernambuco - UFPB; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
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Título: |
Selection of upland cotton for the Brazilian semi-arid region under supplementary irrigation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 19, n. 2, p. 185-192, Apr./July 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to select cotton genotypes adapted to semi-arid conditions grown with supplementary irrigation. Two experiments were carried out between March and July 2016 in Serra Talhada- Pernambuco and Apodi-Rio Grande do Norte, Brazil. Eighteen elite genotypes bred by Embrapa Cotton and two controls (BRS 286 and BRS 336) were evaluated under drip and sprinkler irrigation. The experiment consisted of a randomized complete block design with four replications. Agronomic and fiber quality traits were evaluated. The data were subjected to individual and combined analysis of variance, and genotypes were selected by the Mulamba and Mock selection index (1978). The genetic parameters evidenced the possibility of significant gains in the selection process of cotton plants. The four genotypes (CNPA 2006-3052, CNPA 2004-266, CNPA 2006-1073, and CNPA 2005-125) with highest total genetic gains for the studied traits were considered the most promising. |
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Thesagro: |
Gossypium Hirsutum. |
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Thesaurus NAL: |
Fiber quality; Selection index; Yields. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208774/1/Selection-of-upland-cotton.pdf
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Marc: |
LEADER 01671naa a2200229 a 4500
001 2118756
005 2020-01-20
008 2019 bl uuuu u00u1 u #d
100 1 $aCARVALHO, J. F. de
245 $aSelection of upland cotton for the Brazilian semi-arid region under supplementary irrigation.$h[electronic resource]
260 $c2019
520 $aThis study aimed to select cotton genotypes adapted to semi-arid conditions grown with supplementary irrigation. Two experiments were carried out between March and July 2016 in Serra Talhada- Pernambuco and Apodi-Rio Grande do Norte, Brazil. Eighteen elite genotypes bred by Embrapa Cotton and two controls (BRS 286 and BRS 336) were evaluated under drip and sprinkler irrigation. The experiment consisted of a randomized complete block design with four replications. Agronomic and fiber quality traits were evaluated. The data were subjected to individual and combined analysis of variance, and genotypes were selected by the Mulamba and Mock selection index (1978). The genetic parameters evidenced the possibility of significant gains in the selection process of cotton plants. The four genotypes (CNPA 2006-3052, CNPA 2004-266, CNPA 2006-1073, and CNPA 2005-125) with highest total genetic gains for the studied traits were considered the most promising.
650 $aFiber quality
650 $aSelection index
650 $aYields
650 $aGossypium Hirsutum
700 1 $aCAVALCANTI, J. J. V.
700 1 $aFARIAS, F. J. C.
700 1 $aRAMOS, J. P. C.
700 1 $aQUEIROZ, D. R.
700 1 $aSANTOS, R. C. dos
773 $tCrop Breeding and Applied Biotechnology$gv. 19, n. 2, p. 185-192, Apr./July 2019.
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Registro original: |
Embrapa Algodão (CNPA) |
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