| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
18/11/2014 |
|
Data da última atualização: |
18/11/2014 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BEARSON, B. L.; BEARSON, S. M. D.; KICH, J. D.; LEE, I. S. |
|
Afiliação: |
BRADLEY L. BEARSON, USDA. ARS. National Laboratory for Agriculture and the Environment.; SHAWN MICHELLE DUNKIN BEARSON, USDA. ARS. National Animal Disease center.; JALUSA DEON KICH, CNPSA; IN SOO LEE, Hannam university. Departament of Biological Sciences and Biotechnology. |
|
Título: |
An rfaH mutant of salmonella enterica serovar typhimurium is attenuated in swine and reduces intestinal colonization, fecal shedding, and disease severity due to virulent salmonella typhimurium. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Frontiers in Veterinary Science, Lausana, v. 1, p. 1-7, 2014. |
|
DOI: |
10.3389/fvets.2014.00009 |
|
Idioma: |
Português |
|
Conteúdo: |
Swine are often asymptomatic carriers of Salmonella spp., and interventions are needed to limit colonization of swine to enhance food safety and reduce environmental contamination. We evaluated the attenuation and potential vaccine use in pigs of a Salmonella enterica serovar Typhimurium mutant of rfaH, the gene encoding the RfaH antiterminator that prevents premature termination of long mRNA transcripts. Pigs inoculated with wildtype S.Typhimurium exhibited a significant elevation in average body temperature (fever) at 1 and 2 days post-inoculation; rfaH-inoculated pigs did not (nD5/group). During the 7-day trial, a significant reduction of Salmonella in the feces, tonsils, and cecum were observed in the rfaH-inoculated pigs compared to wild-type inoculated pigs. To determine whether vaccination with the rfaH mutant could provide protection against wild-type S.Typhimurium challenge, two groups of pigs (nD14/group) were intranasally inoculated with either the rfaH mutant or a PBS placebo at 6 and 8weeks of age and challenged with the parental, wild-type S. Typhimurium at 11weeks of age. The average body temperature was significantly elevated in the mock-vaccinated pigs at 1 and 2 days post-challenge, but not in the rfaH-vaccinated pigs. Fecal shedding at 2 and 3 days post-challenge and colonization of intestinal tract tissues at 7 days post-challenge by wild-type S. Typhimurium was significantly reduced in the rfaH-vaccinated pigs compared to mock-vaccinated pigs. Serological analysis using the IDEXX HerdChek Swine Salmonella Test Kit indicated that vaccination with the rfaH mutant did not stimulate an immune response against LPS.These results indicate that vaccination of swine with the attenuated rfaH mutant confers protection against challenge with virulent S.Typhimurium but does not interfere with herd level monitoring for Salmonella spp., thereby allowing for differentiation of infected from vaccinated animals (DIVA). MenosSwine are often asymptomatic carriers of Salmonella spp., and interventions are needed to limit colonization of swine to enhance food safety and reduce environmental contamination. We evaluated the attenuation and potential vaccine use in pigs of a Salmonella enterica serovar Typhimurium mutant of rfaH, the gene encoding the RfaH antiterminator that prevents premature termination of long mRNA transcripts. Pigs inoculated with wildtype S.Typhimurium exhibited a significant elevation in average body temperature (fever) at 1 and 2 days post-inoculation; rfaH-inoculated pigs did not (nD5/group). During the 7-day trial, a significant reduction of Salmonella in the feces, tonsils, and cecum were observed in the rfaH-inoculated pigs compared to wild-type inoculated pigs. To determine whether vaccination with the rfaH mutant could provide protection against wild-type S.Typhimurium challenge, two groups of pigs (nD14/group) were intranasally inoculated with either the rfaH mutant or a PBS placebo at 6 and 8weeks of age and challenged with the parental, wild-type S. Typhimurium at 11weeks of age. The average body temperature was significantly elevated in the mock-vaccinated pigs at 1 and 2 days post-challenge, but not in the rfaH-vaccinated pigs. Fecal shedding at 2 and 3 days post-challenge and colonization of intestinal tract tissues at 7 days post-challenge by wild-type S. Typhimurium was significantly reduced in the rfaH-vaccinated pigs compared to mock-vaccinated pigs. Serologica... Mostrar Tudo |
|
Thesagro: |
Bacteriologia; Salmonella; Suíno. |
|
Thesaurus Nal: |
Bacteriology. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02702naa a2200217 a 4500
001 2000432
005 2014-11-18
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3389/fvets.2014.00009$2DOI
100 1 $aBEARSON, B. L.
245 $aAn rfaH mutant of salmonella enterica serovar typhimurium is attenuated in swine and reduces intestinal colonization, fecal shedding, and disease severity due to virulent salmonella typhimurium.$h[electronic resource]
260 $c2014
520 $aSwine are often asymptomatic carriers of Salmonella spp., and interventions are needed to limit colonization of swine to enhance food safety and reduce environmental contamination. We evaluated the attenuation and potential vaccine use in pigs of a Salmonella enterica serovar Typhimurium mutant of rfaH, the gene encoding the RfaH antiterminator that prevents premature termination of long mRNA transcripts. Pigs inoculated with wildtype S.Typhimurium exhibited a significant elevation in average body temperature (fever) at 1 and 2 days post-inoculation; rfaH-inoculated pigs did not (nD5/group). During the 7-day trial, a significant reduction of Salmonella in the feces, tonsils, and cecum were observed in the rfaH-inoculated pigs compared to wild-type inoculated pigs. To determine whether vaccination with the rfaH mutant could provide protection against wild-type S.Typhimurium challenge, two groups of pigs (nD14/group) were intranasally inoculated with either the rfaH mutant or a PBS placebo at 6 and 8weeks of age and challenged with the parental, wild-type S. Typhimurium at 11weeks of age. The average body temperature was significantly elevated in the mock-vaccinated pigs at 1 and 2 days post-challenge, but not in the rfaH-vaccinated pigs. Fecal shedding at 2 and 3 days post-challenge and colonization of intestinal tract tissues at 7 days post-challenge by wild-type S. Typhimurium was significantly reduced in the rfaH-vaccinated pigs compared to mock-vaccinated pigs. Serological analysis using the IDEXX HerdChek Swine Salmonella Test Kit indicated that vaccination with the rfaH mutant did not stimulate an immune response against LPS.These results indicate that vaccination of swine with the attenuated rfaH mutant confers protection against challenge with virulent S.Typhimurium but does not interfere with herd level monitoring for Salmonella spp., thereby allowing for differentiation of infected from vaccinated animals (DIVA).
650 $aBacteriology
650 $aBacteriologia
650 $aSalmonella
650 $aSuíno
700 1 $aBEARSON, S. M. D.
700 1 $aKICH, J. D.
700 1 $aLEE, I. S.
773 $tFrontiers in Veterinary Science, Lausana$gv. 1, p. 1-7, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 227 | |
| 81. |  | VIANCELLI, A.; KUNZ, A.; FONGARO, G.; KICH, J. D.; BARARDI, C. R. M.; SUZIN, L. Pathogen inactivation by hydrated lime addition in swine wastewater for reuse purposes. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 4., 2015, Rio de Janeiro, RJ. Anais... Brasília: Embrapa, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
|    |
| 82. | | VIANCELLI, A.; KUNZ, A.; FONGARO, G.; KICH, J. D.; BARARDI, C. R. M.; SUZIN, L. Pathogen inactivation and the chemical removal of phosphorus from swine wastewater. Water, Air and Soil Pollution, v. 226, p. 263-271, 2015| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
|  |
| 85. | | PISSETTI, C.; WERLANG, G. O.; BIESUS, L. L.; KICH, J. D.; CARDOSO, M. R. de I. Detecção de salmonella entErica e listeria monocytogenes em carcaças suínas na etapa de pré-resfriamento. Acta Scientiae Veterinariae, Porto Alegre, v. 40, n. 2, p. 1071, 2012. Projeto: 11.11.11.111.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 87. | | PISSETTI, C.; KICH, J. D.; CARDOSO, M.; COLDEBELLA, A.; KETTERMANN, C. A.; FERRAZ, S. M. Efeito de diferentes tratamentos em porções de carcaças de suínos contaminadas superficialmente com Salmonella typhimuriam. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 15., 2011, Fortaleza. Anais de palestras e resumos. Fortaleza: Abraves, 2011. 1 CD-ROM. Projeto: 04.10.06.001.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 90. | | ZANOTTO, D. L.; KICH, J. D.; BELLAVER, C.; SANTIANI, M. J.; LOCATELLI, C.; TRIQUES, N. Farinha de carne e ossos com resíduo flotado de efluentes: avaliação da presença de contaminantes microbiológicos. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 13., 2007, Florianópolis. Anais... Concórdia: Embrapa Suínos e Aves, 2007. 1 CD-ROM. Projeto n. 03.06.52.300-02| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 93. | | KLEIN, C. S.; REBELATTO, R.; MORES, N.; BORDIN, L. C.; KICH, J. D.; VIZZOTTO, R. Estudo do potencial patogênico de cepas de Mycoplasma hyopneumoniae em suínos In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25, 2009. Porto de Galinhas, PE. Anais... SBM, 2009. Disponível em: http://sbmicrobiologia.org.br/PDF/cdsbm/trabalhos.htm. Acesso em 17/02/2010. Projeto/Plano de Ação: 03.07.51.700-02| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
|  |
| 98. | | SCHWARZ, P.; SEYBOTH, L.; ROMEIRO, C.; CORBELLINI, L. G.; CALVEYRA, J.; KICH, J. D.; CARDOSO, M. Risk factors for the herd-level seroprevalence of Salmonella in swine production system in Southern Brazil: preliminary data. In: INTERNATIONAL PIG VETERINARY SOCIETY, 20., 2008, Durban, South Africa. Proceedings. Durban: IPVS, 2008. p. 324 Projeto/Plano de Ação: 02.06.60400-04.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 99. | | SCHWARZ, P.; COLDEBELLA, A.; CALVEYRA, J.; KICH, J. D.; HERTZ, A.; CARDOSO, M. Simulation of intervention models for the control of Salmonella sp. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 22., 2012, Jeju. Proceedings... Jeju: IPVS, 2012. p. 846. Projeto/Plano de Ação: 04.10.60100-03.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| Registros recuperados : 227 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
