Data/hora: 03/06/2024 13:06:10

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 21/09/2017
Data da última atualização: | 21/09/2017
Autoria: | BEARSON, B. L.; BEARSON, S. M. D.; BRUNELLE, B. W.; BAYLES, D. O.; LEE, I. S.; KICH, J. D.
Afiliação: | BRADLEY L. BEARSON, USDA/ARS/National Laboratory for Agriculture and the Environment; SHAWN M. D. BEARSON, USDA/ARS/National Animal Disease Center; BRIAN W. BRUNELLE, USDA/ARS/National Animal Disease Center; DARRELL O. BAYLES, USDA/ARS/National Animal Disease Center; IN SOO LEE, Hannam University; JALUSA DEON KICH, CNPSA.
Título: | Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.
Ano de publicação: | 2017
Fonte/Imprenta: | Journal of Medical Microbiology, v. 66, p. 651-661, 2017.
DOI: | 10.1099/jmm.0.000482
Idioma: | Inglês
Conteúdo: | Abstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Conclusion. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.
Thesagro: | Salmonella Typhimurium; Suinocultura; Vacina; Virologia.
Categoria do assunto: | --
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (21091-1) - UPC - AP - DD -  -  -  - DIS