Data/hora: 19/06/2024 13:03:03

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	13/06/2016	
Data da última atualização: 	13/06/2016	
Autoria: 	ALMEIDA, F. de; MEDEIROS, M. I. C.; KICH, J. D.; FALCÃO, J. P.	
Afiliação: 	FERNANDA DE ALMEIDA, USP/Faculdade de Ciências farmacêuticas de Ribeirão Preto; MARTA INÊS CAZENTINI MEDEIROS, Instituto Adolfo Lutz; JALUSA DEON KICH, CNPSA; JULIANA PFRIMER FALCÃO, USP/Faculdade de Ciências Farmacêuticas de Ribeirão Preto.	
Título: 	Virulence-associated genes, antimicrobial resistance and molecular typing of salmonella typhimurium strains isolated from swine from 2000 to 2012 in Brazil.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Journal of Applied Microbiology, v. 120, n. 6, p. 1677-1690, 2016.	
DOI: 	10.1111/jam.13110	
Idioma: 	Inglês	
Conteúdo: 	Abstract Aims: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. Methods and Results: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 778% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. Conclusions: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. Significance and Impact of the Study: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide.	
Thesagro: 	Bactéria; Microbiologia; Patogenicidade; Produção animal; Salmonella Typhimurium; Suíno.	
Thesaurus Nal: 	Swine production; Virulence.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (20670-1) - UPC - AP - DD -  -  -  - DIS