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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
19/10/2016 |
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Data da última atualização: |
20/10/2016 |
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Autoria: |
CHAI, K.; MILLIGAN, L. P.; KENNEDY, P. M.; MATHISON, G. W. |
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Título: |
Effects of cold exposure and plant species on forage intake, chewing behavior and digesta particle size in sheep. |
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Ano de publicação: |
1985 |
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Fonte/Imprenta: |
Canadian Journal of Animal Science, v. 65, n. 1, p. 69-76, Mar. 1985. |
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DOI: |
10.4141/cjas85-007 |
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Idioma: |
Inglês |
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Conteúdo: |
Three closely shorn sheep were allotted to each of three diets of chopped hay prepared from red clover, reed canarygrass and bromegrass, respectively. The animals were housed at 25, 10 and 5 °C during three periods of 28 days. For the final 8 days of each period, each individual sheep was restricted to 90% of its voluntary feed consumption and hay was given at intervals of 2 h. Eating and chewing behavior, rumen fluid kinetics and digesta particle size were determined. Voluntary intake of clover hay was greater, but chews during eating and rumination were less than for sheep consuming grass hay. The small particle fractions present in the duodenum and rumination activity were greater in the sheep fed grasses. Feed intake, rumination chews and duration, and digesta particle size in the duodenum were increased in sheep housed at 5 °C. Eating duration and chews, particle size of rumen digesta and rumen fluid volume were not affected by cold stress, but fluid retention time was reduced, while daily fluid flow from the rumen tended to increase. Key words: Forage intake, cold stress, chewing behavior, digesta particle size, fluid passage |
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Palavras-Chave: |
Digesta particle size; Feed crops; Feeding habits; Tamanho de partículas. |
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Thesagro: |
Digestão; Hábito alimentar; Ingestão; Mastigação; Nutrição animal; Ovino; Planta forrageira; Stress. |
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Thesaurus Nal: |
Animal nutrition; Cold; Digesta; Particle size; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02180naa a2200373 a 4500
001 2054960
005 2016-10-20
008 1985 bl uuuu u00u1 u #d
024 7 $a10.4141/cjas85-007$2DOI
100 1 $aCHAI, K.
245 $aEffects of cold exposure and plant species on forage intake, chewing behavior and digesta particle size in sheep.
260 $c1985
520 $aThree closely shorn sheep were allotted to each of three diets of chopped hay prepared from red clover, reed canarygrass and bromegrass, respectively. The animals were housed at 25, 10 and 5 °C during three periods of 28 days. For the final 8 days of each period, each individual sheep was restricted to 90% of its voluntary feed consumption and hay was given at intervals of 2 h. Eating and chewing behavior, rumen fluid kinetics and digesta particle size were determined. Voluntary intake of clover hay was greater, but chews during eating and rumination were less than for sheep consuming grass hay. The small particle fractions present in the duodenum and rumination activity were greater in the sheep fed grasses. Feed intake, rumination chews and duration, and digesta particle size in the duodenum were increased in sheep housed at 5 °C. Eating duration and chews, particle size of rumen digesta and rumen fluid volume were not affected by cold stress, but fluid retention time was reduced, while daily fluid flow from the rumen tended to increase. Key words: Forage intake, cold stress, chewing behavior, digesta particle size, fluid passage
650 $aAnimal nutrition
650 $aCold
650 $aDigesta
650 $aParticle size
650 $aSheep
650 $aDigestão
650 $aHábito alimentar
650 $aIngestão
650 $aMastigação
650 $aNutrição animal
650 $aOvino
650 $aPlanta forrageira
650 $aStress
653 $aDigesta particle size
653 $aFeed crops
653 $aFeeding habits
653 $aTamanho de partículas
700 1 $aMILLIGAN, L. P.
700 1 $aKENNEDY, P. M.
700 1 $aMATHISON, G. W.
773 $tCanadian Journal of Animal Science$gv. 65, n. 1, p. 69-76, Mar. 1985.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/12/2014 |
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Data da última atualização: |
12/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A. |
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Afiliação: |
FURGS; FURGS; RODRIGO DA ROCHA FRAGOSO, CPAC; WAGNER ALEXANDRE LUCENA, CNPA; IAPAR; UNIVERSITY OF CALDAS, COLOMBIA; INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, FRANCE; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN. |
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Título: |
Molecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Gene, v. 553, n. 1, p. 7-16, Dec. 2014. |
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DOI: |
10.1016/j.gene.2014.09.050 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies. MenosAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica. |
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Thesagro: |
Amido; Curculionideo; Hidrolise; Praga de planta. |
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Thesaurus NAL: |
Curculionidae; Enzymatic hydrolysis; Gene expression; Insect pests; Starch. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114033/1/34279.pdf
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Marc: |
LEADER 02768naa a2200385 a 4500
001 2003023
005 2015-02-12
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.gene.2014.09.050$2DOI
100 1 $aBEZERRA, C. A.
245 $aMolecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei.
260 $c2014
520 $aAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies.
650 $aCurculionidae
650 $aEnzymatic hydrolysis
650 $aGene expression
650 $aInsect pests
650 $aStarch
650 $aAmido
650 $aCurculionideo
650 $aHidrolise
650 $aPraga de planta
653 $aExpressão gênica
700 1 $aMACEDO, L. L. P.
700 1 $aAMORIM, T. M. L.
700 1 $aSANTOS, V. O.
700 1 $aFRAGOSO, R. da R.
700 1 $aLUCENA, W. A.
700 1 $aMENEGUIM, A. M.
700 1 $aVALENCIA-JIMENEZ, A.
700 1 $aENGLER, G.
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
700 1 $aFREIRE, E. V. S. A.
773 $tGene$gv. 553, n. 1, p. 7-16, Dec. 2014.
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Embrapa Cerrados (CPAC) |
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