Data/hora: 22/06/2024 03:04:07

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 11/12/2009
Data da última atualização: | 25/02/2010
Autoria: | KOBAYASHI, A. K.; RODRIGUEZ, M. A. D.; SOUZA JUNIOR, M. T.; KEMA, G. H. J.
Afiliação: | Adilson Kenji Kobayashi, CPAMN/PRI; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; MANOEL TEIXEIRA SOUZA JUNIOR, Labex Europa; Gert H. J. Kema, PRI.
Título: | Generation of Fusarium f. sp. passiflorae strains expressing reporter genes.
Ano de publicação: | 2009
Fonte/Imprenta: | Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento.
ISSN: | 1982-5676
Idioma: | Inglês
Notas: | Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 607.
Conteúdo: | In order generate transgenic Fusarium oxysporum f. sp. passiflorae (Fop) straisn, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Transformation procedures mediated by Agrobacterium tumefaciens (strain LBA1 100) were carried out using a 106 conidia.mL-¹ suspension of the Fop isolate CNPMF08-01p. After the co-cultivation period, the cultures were transferred to seletive medium containing 100 mg.mL-¹ hygromycin. Transgenic events expressing gfp of dsred proteins were identified by fluorescent microscopy observations after 1 week. Such fluorescent protein-labeled strains may constitute a useful tool in improving the understanding this pathosystem, specially, in the early stages of the infection process.
Thesagro: | Doença de Planta.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (26352-1) - UPC - AP - PP -  -  -  - PCT