Data/hora: 20/06/2024 07:57:12

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 19/08/2005
Data da última atualização: | 19/08/2019
Autoria: | CAIXETA, E. T.; BORÉM, A.; KELLY, J. D.
Afiliação: | EVELINE TEIXEIRA CAIXETA; ALUIZIO BOREM; JAMES D KELLY.
Título: | Development of microsatellite markers based on BAC common bean clones.
Ano de publicação: | 2005
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 2, p. 125-133, June 2005.
Idioma: | Inglês
Conteúdo: | The eukaryotic genome owns simple sequence repeats in tandem, called microsatellites or SSR. The microsatellite-flanking DNA sequences are generally conserved among the plants of a same species, allowing that primers complementary to these regions may be used to amplify SSR-containing fragments. These repetitive regions are therefore used as molecular markers. In the present study, some microsatellite markers were developed using bacterial artificial chromosome (BAC) clones as DNA source. Four BAC clones were sub-cloned, hybridized with three different probes and the positive clones were sequenced. Specific primers were designed and tested in common bean (Phaseolus vulgaris) genotypes. Twenty-one pairs of primers amplified single and well-defined bands. One to six alleles per locus were generated, demonstrating the high polymorphism of these markers. This set of developed microsatellite markers together with the other available markers are an important tool in the breeding and genetic study of common bean.
Palavras-Chave: | SSR.
Thesagro: | Feijão; Marcador Molecular; Phaseolus Vulgaris.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (22898-1) - ADD - AP - DD - 2005 -  - 2005 - DIS