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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
08/10/2001 |
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Data da última atualização: |
08/10/2001 |
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Autoria: |
KELEMU, S.; WHITE JUNIOR, J. F.; MUNOZ, F.; TAKAYAMA, Y. |
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Título: |
An endophyte of the tropical forage grass Brachiaria brizantha: Isolating, identifying, and characterizing the fungus, and determining its antimycotic properties. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Canadian Journal of Microbiology, Ottawa, v. 47, p. 55-62, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
Brachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic c4 grass, that are commercially important forage grasses in Tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically significant. The fungus was identified as Acremonium implicatum (J. Gilman & E.V. Abott). 18S rDNA and ITS rDNA sequences were used to characterize isolates of the endophyte, and showed that they belonged to the Acremonium genus, being close to A. strictum and A. kiliense. Using the randow amplified polymorphic DNA (RAPD) technique, involving involving arbitrary primers of 10 bases, we showed that the isolates were highly similar to each other. Antiserum produced from a monoconidial culture of A. implicatum isolated from B. brizantha 6780, differentiated the isolates consistently in line with the DNA data. When we compared endophyte-free with endophyte-infected B.brizantha CIAT 6780 plants, both artificially inoculated with the pathogenic Drechslera fungus, we found that the endophyte-infected plants had fewer and smaller lesions than did the endophyte-free plants. Sporulation of Drechslera sp. on artifiacially inoculated leaf sheath tissues was also much less on tissue infected with the endophyte. |
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Palavras-Chave: |
Acremonium implicatum; Caracterizacao; Caracterization; Deoxyribonucleic acid; Diseases; Endofito; Identification; In vitro; Inoculation. |
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Thesagro: |
Brachiaria Brizantha; Controle Biológico; DNA; Doença; Gramínea Forrageira; Gramineae; Identificação; Inoculação. |
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Thesaurus Nal: |
biological control; endophytes. |
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Categoria do assunto: |
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Marc: |
LEADER 02450naa a2200385 a 4500
001 1621269
005 2001-10-08
008 2001 bl --- 0-- u #d
100 1 $aKELEMU, S.
245 $aAn endophyte of the tropical forage grass Brachiaria brizantha$bIsolating, identifying, and characterizing the fungus, and determining its antimycotic properties.
260 $c2001
520 $aBrachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic c4 grass, that are commercially important forage grasses in Tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically significant. The fungus was identified as Acremonium implicatum (J. Gilman & E.V. Abott). 18S rDNA and ITS rDNA sequences were used to characterize isolates of the endophyte, and showed that they belonged to the Acremonium genus, being close to A. strictum and A. kiliense. Using the randow amplified polymorphic DNA (RAPD) technique, involving involving arbitrary primers of 10 bases, we showed that the isolates were highly similar to each other. Antiserum produced from a monoconidial culture of A. implicatum isolated from B. brizantha 6780, differentiated the isolates consistently in line with the DNA data. When we compared endophyte-free with endophyte-infected B.brizantha CIAT 6780 plants, both artificially inoculated with the pathogenic Drechslera fungus, we found that the endophyte-infected plants had fewer and smaller lesions than did the endophyte-free plants. Sporulation of Drechslera sp. on artifiacially inoculated leaf sheath tissues was also much less on tissue infected with the endophyte.
650 $abiological control
650 $aendophytes
650 $aBrachiaria Brizantha
650 $aControle Biológico
650 $aDNA
650 $aDoença
650 $aGramínea Forrageira
650 $aGramineae
650 $aIdentificação
650 $aInoculação
653 $aAcremonium implicatum
653 $aCaracterizacao
653 $aCaracterization
653 $aDeoxyribonucleic acid
653 $aDiseases
653 $aEndofito
653 $aIdentification
653 $aIn vitro
653 $aInoculation
700 1 $aWHITE JUNIOR, J. F.
700 1 $aMUNOZ, F.
700 1 $aTAKAYAMA, Y.
773 $tCanadian Journal of Microbiology, Ottawa$gv. 47, p. 55-62, 2001.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 17 | |
| 2. |  | CHAKRABORTY, S.; PERROT, R.; CHARCHAR, M. J. D`A.; FERNANDES, C. D.; KELEMU, S. Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. II. Genetic and pathogenic diversity in isolates of Colletotrichum gloeosporioides from eight species of Stylosanthes. Tropical Grasslands, Brisbane, v.31, n.5, p.393-401, Nov. 1997. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | CHAKRABORTY, S.; PERROT, R.; CHARCHAR, M. J. D' A.; FERNANDES, C. D.; KELEMU, S. Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. II. Genetic and pathogenic diversity in isolates of Colletotrichum gloeosporioides from eight species of Stylosanthes. Tropical Grasslands, Queensland, v.31, p.393-401, 1997.| Biblioteca(s): Embrapa Cerrados. |
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| 4. | | CAMERON, D. F.; CHARCHAR, M. J. D`A.; FERNANDES, C. D.; KELEMU, S.; CHAKRABORTY, S. Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. III. Field evaluation of Stylosanthes species for anthracnose resistance in their centre of diversity. Tropical Grasslands, Brisbane, v.31, n.5, p.402-407, Nov. 1997. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | CAMERON, D. F.; CHARCHAR, M. J. D' A.; FERNANDES, C. D.; KELEMU, S.; CHAKRABORTY, S. Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. III. Field evaluation of Stylosanthes species for anthracnose resistance in their centre of diversity. Tropical Grasslands, Queensland, v. 31, p. 402-407, 1997.| Biblioteca(s): Embrapa Cerrados. |
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| 6. | | CHAKRABORTY, S.; FERNANDES, C. D.; CHARCHAR, M. J. D'A.; KELEMU, S.; CAMERON, D. F. Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. IV. Epidemiology of Stylosanthes anthracnose at the center of host-pathogen diversity. Tropical Grasslands, Brisbane, v.31, n.5, p.409-416, Nov. 1997.| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | CHAKRABORTY, S.; FERNANDES, C. D.; CHARCHAR, M. J. D'A.; KELEMU, S.; CAMERON, D. F. Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. IV. Epidemiology of Stylosanthes anthracnose at the centre of host-pathogen diversity. Tropical Grasslands, Queensland, v.31, p.408-416, 1997.| Biblioteca(s): Embrapa Cerrados. |
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| 8. | | CHAKRABORTY, S.; PERROT, R.; CHARCHAR, M. J. D'A.; FERNANDES, C. D.; KELEMU, S. Variation in the Stylosanthes anthracnose pathogen: implications for australian cultivars. In: INTERNATIONAL GRASSLAND CONGRESS, 18., 1997, Winnipeg, Saskatoon. Proceedings... [S.l.: s.n., 1997?]. p. 13-1 / 13-2. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 11. | | CHAKRABORTY, S.; PERROT, R.; CHARCHAR, M. J. d' A.; FERNANDES, C. D.; KELEMU, S. Pathogenic & genetic variation in Colletotrichum gloeosporioides infecting Stylosanthes spp.: implications for Australian cultivars. Phytopathology, St. Paul, v. 87, n. 6, p. S16, 1997. Apresentado no "APS Annual Meeting, 1997, Rochester, New York".| Biblioteca(s): Embrapa Cerrados. |
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| 12. | | WEEDS, P. CHAKRABORTY, S.; FERNANDES, C. D.; CHARCHAR, M. J.; RAMESH; C. R.; GUODAO, L.; KELEMU, S. Genetic diversity i the anthracnose pathogen infecting Stylosanthes in Brazil, India and China. In: INTERNATIONAL GRASSLAND CONGRESS, 19., 2001, Sao Pedro, SP. Grassland ecosystems: an outlook into the 21st century: proceedings. Piracicaba: FEALQ, 2001. p. 232-234.| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | WEEDS, P.; CHAKRABORTY, S.; FERNANDES, C. D.; CHARCHAR, M. J.; RAMESH, C. R.; GUODAO, L.; KELEMU, S. Genetic diversity in the anthracnose pathogen infecting Stylosanthes in Brazil, India and China. In: INTERNATIONAL GRASSLAND CONGRESS, 19., 2001, Sao Pedro, SP. Proceedings... Piracicaba: FEALQ, 2001. p. 232-234.| Biblioteca(s): Embrapa Cerrados; Embrapa Gado de Corte. |
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| 14. | | WEEDS, P. L.; CHAKRABORTY, S.; FERNANDES, C. D.; CHARCHAR, M. J. d'A.; RAMESH, C. R.; KEXIAN, Y.; KELEMU, S. Genetic diversity in Colletotrichum gloeosporioides from Stylosanthes spp. at centers of origin and utilization. Phytopathology, St. Paul, v. 93, n. 2, p. 176-185, 2003.| Biblioteca(s): Embrapa Cerrados. |
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| 15. | | KELEMU, S.; BADEL, J. L.; MORENO, C. X.; MILES, J. W.; CHAKRABORTY, S.; FERNANDES, C. D.; CHARCAR, M. J. D`A. Biodeversity, epidemiology and virulence of colletotrichum gloeosporioides. I.Genetic and patrhogenica diversituy in colletotrichum gloesporioides isolates from Stylosanthes guianensis. Tropical Grasslands, Brisbane, v.31, n.5, p.387-392, Nov. 1997. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | KELEMU, S.; BADEL, J. L.; MORENO, C. X.; MILES, J. W.; CHAKRABORTY, S.; FERNANDES, C. D.; CHARCHAR, M. J. D' A. Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. I. genetic and pathogenic diversity in Colletotrichum gloeosporioides isolates from Stylosanthes guianensis. Tropical Grasslands, Queensland, v. 31, p. 387-392, 1997.| Biblioteca(s): Embrapa Cerrados. |
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| 17. | | KELEMU, S.; SKINNER, D.; BADEL; J. L.; MORENO, C. X.; RODRIGUEZ, M. X.; FERNANDES, C. D.; CHARCHAR, M. J.; CHAKRABORTY, S. Genetic diversity in South American Colletottrichum goleosporioides isolates from Stylosanthes guianensis, a tropical forage legume. European Journal of Plant Pathology, v. 105, p. 261-272, 1999.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 17 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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