Registro Completo |
Biblioteca(s): |
Ebooks. |
Data corrente: |
15/08/2008 |
Data da última atualização: |
04/02/2013 |
Autoria: |
KAY, B. K.; WINTER, J.; McCAFFERTY, J. D. |
Afiliação: |
Brian K. Kay; Jill Winter; John Dr. Mccafferty. |
Título: |
Phage display of peptides and proteins a laboratory manual |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
San Diego : Academic Press, 1996. |
Páginas: |
xxii, 344 p. |
Descrição Física: |
ill. (some col.) ;24 cm. |
ISBN: |
9780124023802 |
Idioma: |
Inglês |
Conteúdo: |
Foreword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization.Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references. MenosForeword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization.Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Fe... Mostrar Tudo |
Palavras-Chave: |
Affinity; Antistoffen; Combinatorische chemie; Eiwitten; Genexpressie; Peptiden; ProteinsBiotechnology. |
Thesaurus Nal: |
bacteriophages; biotechnology; chromatography; peptides; proteins. |
Categoria do assunto: |
-- |
URL: |
https://www.sciencedirect.com/science/book/9780124023802
|
Marc: |
LEADER 02509nam a2200301 a 4500 001 1711319 005 2013-02-04 008 1996 bl uuuu u0uu1 u #d 020 $a9780124023802 100 1 $aKAY, B. K. 245 $aPhage display of peptides and proteins a laboratory manual$h[electronic resource] 260 $aSan Diego : Academic Press$c1996 300 $axxii, 344 p. $cill. (some col.) ;24 cm. 520 $aForeword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization.Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Key Features * Prepared by experts in the field * Provides proven laboratory protocols, troubleshooting, and tips * Includes maps, sequences, and sample data * Contains extensive and up-to-date references. 650 $abacteriophages 650 $abiotechnology 650 $achromatography 650 $apeptides 650 $aproteins 653 $aAffinity 653 $aAntistoffen 653 $aCombinatorische chemie 653 $aEiwitten 653 $aGenexpressie 653 $aPeptiden 653 $aProteinsBiotechnology 700 1 $aWINTER, J. 700 1 $aMcCAFFERTY, J. D.
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