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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
15/03/2021 |
Data da última atualização: |
10/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BOARI, A. de J.; QUADROS, A. F. F.; KAUFFMANN, C. M.; PANTOJA, K. F. C.; GOMES JUNIOR, R. A.; FREIRE FILHO, F. R.; OLIVEIRA, R. P. de; CORDOVIL, G. A.; GAVINHO, B. E. S.; KITAJIMA, E. W.; BLAWID, R.; NAGATA, T. |
Afiliação: |
ALESSANDRA DE JESUS BOARI, CPATU; AYANE F. F. QUADROS, UFRA; CATERYNNE M. KAUFFMANN, UFRA; KÉSSIA F. C. PANTOJA, Univesidade Estadual de São Paulo; RUI ALBERTO GOMES JUNIOR, CPATU; FRANCISCO RODRIGUES FREIRE FILHO, CPATU; RAIMUNDO PARENTE DE OLIVEIRA, CPATU; GABRIELE A. CORDOVIL, UFRA; BRENDA E. S. GAVINHO, UFRA; ELLIOT W. KITAJIMA, ESALQ/USP; ROSANA BLAWID, Universidade Federal Rural de Pernambuco; TATSUYA NAGATA, UNB. |
Título: |
Near-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Tropical Plant Pathology, v. 46, n. 4, p. 487-491, Aug. 2021. |
DOI: |
https://doi.org/10.1007/s40858-021-00420-w |
Idioma: |
Inglês |
Conteúdo: |
The sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%). MenosThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% ... Mostrar Tudo |
Thesagro: |
Feijão; Genoma; Vigna Unguiculata; Vírus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02717naa a2200313 a 4500 001 2130679 005 2021-08-10 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s40858-021-00420-w$2DOI 100 1 $aBOARI, A. de J. 245 $aNear-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection.$h[electronic resource] 260 $c2021 520 $aThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%). 650 $aFeijão 650 $aGenoma 650 $aVigna Unguiculata 650 $aVírus 700 1 $aQUADROS, A. F. F. 700 1 $aKAUFFMANN, C. M. 700 1 $aPANTOJA, K. F. C. 700 1 $aGOMES JUNIOR, R. A. 700 1 $aFREIRE FILHO, F. R. 700 1 $aOLIVEIRA, R. P. de 700 1 $aCORDOVIL, G. A. 700 1 $aGAVINHO, B. E. S. 700 1 $aKITAJIMA, E. W. 700 1 $aBLAWID, R. 700 1 $aNAGATA, T. 773 $tTropical Plant Pathology$gv. 46, n. 4, p. 487-491, Aug. 2021.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registros recuperados : 25 | |
21. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BOARI, A. de J.; CORDOVIL, G. A.; KAUFFMANN, C. M.; GAVINHO, B. E. S.; QUADROS, A. F. F.; PANTOJA, K. F. C.; GOMES JUNIOR, R. A.; FREIRE FILHO, F. R.; OLIVEIRA, R. P. de; KITAJIMA, E. W. Bean common mosaic virus infecting manteiguinha cowpea (Vigna unguiculata): seed transmission, evaluation of yield loss, and genetic resistance. Tropical Plant Pathology, v. 47, n. 3, p. 450-455, June 2022.Tipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Amazônia Oriental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
22. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MAEDA, M. H. K.; KOYAMA, L. H. H.; CAMPOS, R. N. S.; KAUFFMANN, C. M.; SOUZA, J. O. de; GILBERTSON, R.; INOUE-NAGATA, A. K.; FREITAS, D. M. S.; NOGUEIRA, D. R. S.; MELO, F. L.; NAGATA, T. First report of watermelon crinkle leaf-associated virus 1 and 2 infecting watermelon (Citrullus lanatus) plants in Brazil. Plant Disease, v. 106, n. 2, p. 773, Feb. 2022.Tipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Hortaliças; Embrapa Semiárido. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
23. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, B. A.; MOTA, H. B. da S.; KAUFFMANN, C. M.; QUEIROZ, P. de S.; BATISTA, A. M. do V.; CÁRDENAS, S. B. dos S.; FREITAS, D. M. S.; NAGATA, T. Identificação de vírus associados a cucurbitáceas por sequenciamento de alto desempenho. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 649.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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24. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CASTRO, L. C.; SAUTER, I. P.; ETHUR, E. M.; KAUFFMANN, C.; DALL'AGNOL, R.; SOUZA, J.; CIBULSKI, S. P.; MUNIZ, A. W.; WEIDLICH, L.; LOHMANN, P. M.; ROEHE, P. M.; GERMANI, J. C.; ROTT, M. B.; VAND DER SAND, S. T. In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites. Revista Brasileira de Plantas Medicinais, Campinas, v. 15, n. 4, p. 589-594, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Amazônia Ocidental. |
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25. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BOARI, A. de J.; QUADROS, A. F. F.; KAUFFMANN, C. M.; PANTOJA, K. F. C.; GOMES JUNIOR, R. A.; FREIRE FILHO, F. R.; OLIVEIRA, R. P. de; CORDOVIL, G. A.; GAVINHO, B. E. S.; KITAJIMA, E. W.; BLAWID, R.; NAGATA, T. Near-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection. Tropical Plant Pathology, v. 46, n. 4, p. 487-491, Aug. 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Amazônia Oriental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
Registros recuperados : 25 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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