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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo; Embrapa Unidades Centrais. |
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Data corrente: |
13/11/2008 |
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Data da última atualização: |
17/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HOMRICH, M. S.; PASSAGLIA, L. M. P.; PEREIRA, J. F.; BERTAGNOLLI, P. F.; SALVADORI, J. R.; NICOLAU, M.; KALTCHUK-SANTOS, E.; ALVES, L. B.; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
Jorge Fernando Pereira, CNPT; PAULO FERNANDO BERTAGNOLLI, CNPT; José Roberto Salvadori, CNPT; MARCIO NICOLAU, CNPT. |
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Título: |
Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 7, p. 801-807, jul. 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The presence of cry1Ac transgene affected neither the development nor the yield of plants. Cytogenetical analysis showed that transgenic plants presented normal karyotype. In detached-leaf bioassay, cry1Ac plants exhibited complete efficacy against A. gemmatalis, whereas negative controls were significantly damaged. Whole-plant feeding assay confirmed a very high protection of cry1Ac against velvetbean caterpillar, while nontransgenic 'IAS 5' plants and homozygous gusA line exhibited 56.5 and 71.5% defoliation, respectively. The presence of cry1Ac transgene doesn't affect the majority of agronomic traits (including yield) of soybean and grants high protection against A. gemmatalis. |
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Palavras-Chave: |
plant resistance to insects; resistência de plantas a insetos; transferência de genes. |
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Thesagro: |
Anticarsia Gemmatalis; Glycine Max; Soja. |
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Thesaurus Nal: |
gene transfer. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPT-2010/11249/1/43n07a03.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2009-09/44846/1/43n07a03.pdf
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Marc: |
LEADER 02410naa a2200301 a 4500
001 1823033
005 2021-08-17
008 2008 bl uuuu u00u1 u #d
100 1 $aHOMRICH, M. S.
245 $aAgronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene.
260 $c2008
520 $aThe objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The presence of cry1Ac transgene affected neither the development nor the yield of plants. Cytogenetical analysis showed that transgenic plants presented normal karyotype. In detached-leaf bioassay, cry1Ac plants exhibited complete efficacy against A. gemmatalis, whereas negative controls were significantly damaged. Whole-plant feeding assay confirmed a very high protection of cry1Ac against velvetbean caterpillar, while nontransgenic 'IAS 5' plants and homozygous gusA line exhibited 56.5 and 71.5% defoliation, respectively. The presence of cry1Ac transgene doesn't affect the majority of agronomic traits (including yield) of soybean and grants high protection against A. gemmatalis.
650 $agene transfer
650 $aAnticarsia Gemmatalis
650 $aGlycine Max
650 $aSoja
653 $aplant resistance to insects
653 $aresistência de plantas a insetos
653 $atransferência de genes
700 1 $aPASSAGLIA, L. M. P.
700 1 $aPEREIRA, J. F.
700 1 $aBERTAGNOLLI, P. F.
700 1 $aSALVADORI, J. R.
700 1 $aNICOLAU, M.
700 1 $aKALTCHUK-SANTOS, E.
700 1 $aALVES, L. B.
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 7, p. 801-807, jul. 2008.
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Registro original: |
Embrapa Trigo (CNPT) |
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Origem |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
06/02/2023 |
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Data da última atualização: |
12/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 3 |
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Autoria: |
LIMA, R.; SILVA, M. V. T.; GOMES, B. A.; MACEDO, E. H. B. C.; SANTANA, M. N.; AMARAL, A. C. F.; SILVA, J. R. A.; CORRÊA, P. G.; GODOY, R. L. de O.; SANTIAGO, M. C. P. de A.; LEITÃO, S. G.; SIMAS, R. C.; CARNEIRO, C. S.; RODRIGUES, I. A. |
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Afiliação: |
RAISSA LIMA, UFRJ; MARCOS VINICIUS T. SILVA, UFRJ; BRENDO A. GOMES, UFRJ; ELLIS HELENA B. C. MACEDO, UFRJ; MICHELE N. SANTANA, UFRJ; ANA CLAUDIA F. AMARAL, Fiocruz; JEFFERSON R. A. SILVA, UFAM; POLLYANE G. CORRÊA, UFAM; RONOEL LUIZ DE OLIVEIRA GODOY, CTAA; MANUELA CRISTINA P DE A SANTIAGO, CTAA; SUZANA G. LEITÃO, UFRJ; ROSINEIDE C. SIMAS, UFG; Universidade Presbiteriana Mackenzie; CARLA S. CARNEIRO, UFRJ; IGOR A. RODRIGUES, UFRJ. |
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Título: |
Chemical Profile and Hematoprotective Activity of Artisanal Jabuticaba (Plinia jabuticaba) Wine and Derived Extracts. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Fermentation, v. 9, n. 2, e157, 2023. |
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DOI: |
https://doi.org/10.3390/fermentation9020157 |
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Idioma: |
Inglês |
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Conteúdo: |
The alcoholic fermentation of jabuticaba berries (Plinia spp.) originates from a beverage with an intense taste and aroma, popularly known as jabuticaba wine (JW). In addition, polyphenols transferred from fruit peels to the final product turn this beverage into a promising source of bioactive agents. Here, the chemical profile and antioxidant potential of artisanal JW and derivative extracts were determined. Volatile organic compounds were determined by HS-SPME/GC-MS analysis. The wine was dried by lyophilization and subjected to liquid-liquid partitioning (water: ethyl acetate), resulting in three fractions (JWF1-3). ABTS•+ and DPPH•+ scavenging assays were performed to evaluate the antioxidant capacity. In addition, the extracts’ hematoprotective activity was evaluated against oxidative stress. Finally, the extracts were analyzed by LC-HRMS/MS. HS-SPME/GC-MS analysis highlighted 1,8-cineole as the main compound that contributes to the camphor/mint flavor. JWF2 and JWF3 displayed the highest antioxidant capacity. JWF2 stood out for preventing oxidative damage in red blood cells at 7.8 μg·mL−1 The maximal protection of ascorbic acid occurred at 8.8 μg·mL−1. The LC-HRMS/MS analysis allowed the annotation of seventeen compounds, most of them with recognized antioxidant activity such as anthocyanins, catechins, flavanols, and phenolic acids. The results presented herein reinforce JW as a pleasant beverage with bioactive potential. |
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Palavras-Chave: |
Compostos orgânicos voláteis; Estresse oxidativo; Hematoproteção; Hematoprotection; Polifenóis. |
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Thesagro: |
Composto Orgânico; Fermentação. |
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Thesaurus NAL: |
Fermentation; Oxidative stress; Polyphenols; Volatile organic compounds. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151509/1/Jabuticaba-Plinia-jabuticaba-Wine-and-Derived-Extracts.pdf
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Marc: |
LEADER 02726naa a2200421 a 4500
001 2151509
005 2024-01-12
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/fermentation9020157$2DOI
100 1 $aLIMA, R.
245 $aChemical Profile and Hematoprotective Activity of Artisanal Jabuticaba (Plinia jabuticaba) Wine and Derived Extracts.$h[electronic resource]
260 $c2023
520 $aThe alcoholic fermentation of jabuticaba berries (Plinia spp.) originates from a beverage with an intense taste and aroma, popularly known as jabuticaba wine (JW). In addition, polyphenols transferred from fruit peels to the final product turn this beverage into a promising source of bioactive agents. Here, the chemical profile and antioxidant potential of artisanal JW and derivative extracts were determined. Volatile organic compounds were determined by HS-SPME/GC-MS analysis. The wine was dried by lyophilization and subjected to liquid-liquid partitioning (water: ethyl acetate), resulting in three fractions (JWF1-3). ABTS•+ and DPPH•+ scavenging assays were performed to evaluate the antioxidant capacity. In addition, the extracts’ hematoprotective activity was evaluated against oxidative stress. Finally, the extracts were analyzed by LC-HRMS/MS. HS-SPME/GC-MS analysis highlighted 1,8-cineole as the main compound that contributes to the camphor/mint flavor. JWF2 and JWF3 displayed the highest antioxidant capacity. JWF2 stood out for preventing oxidative damage in red blood cells at 7.8 μg·mL−1 The maximal protection of ascorbic acid occurred at 8.8 μg·mL−1. The LC-HRMS/MS analysis allowed the annotation of seventeen compounds, most of them with recognized antioxidant activity such as anthocyanins, catechins, flavanols, and phenolic acids. The results presented herein reinforce JW as a pleasant beverage with bioactive potential.
650 $aFermentation
650 $aOxidative stress
650 $aPolyphenols
650 $aVolatile organic compounds
650 $aComposto Orgânico
650 $aFermentação
653 $aCompostos orgânicos voláteis
653 $aEstresse oxidativo
653 $aHematoproteção
653 $aHematoprotection
653 $aPolifenóis
700 1 $aSILVA, M. V. T.
700 1 $aGOMES, B. A.
700 1 $aMACEDO, E. H. B. C.
700 1 $aSANTANA, M. N.
700 1 $aAMARAL, A. C. F.
700 1 $aSILVA, J. R. A.
700 1 $aCORRÊA, P. G.
700 1 $aGODOY, R. L. de O.
700 1 $aSANTIAGO, M. C. P. de A.
700 1 $aLEITÃO, S. G.
700 1 $aSIMAS, R. C.
700 1 $aCARNEIRO, C. S.
700 1 $aRODRIGUES, I. A.
773 $tFermentation$gv. 9, n. 2, e157, 2023.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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