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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/01/2001 |
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Data da última atualização: |
18/01/2001 |
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Autoria: |
KUSTER, H.; PUHLER, A.; PERLICK, A. M. |
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Título: |
Analysis of genes encoding modular nodulins from Vicia hirsuta and Vicia faba. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Plant Science, Ireland, v. 149, p. 1-11, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
The expession of genes encoding modular nodulins was analysed in the Vicia hirsuta and the Vicia faba system. From V. hirsuta root nodules, we isolated two families of transcript sequences designated VhNOD28/32-A and VhNOD28/32-B encoding nodulins homologous to the V. faba nodulins Nvf-28/32 and the Medicago sativa nodulin-25. The modular proteins encoded by the V. hirsuta transcript sequences consisted of N- and C-terminal unique modules flanking two types of repetitive modules. Specific repetitive modules were deleted from individual VhNOD28/32 transcripts. Southern hybridizations indicated the presence of either a small VhNOD28/32 gene family or a single copy gene comprising at least two alleles. VhNOD28/32 transcripts were expressed specifically in root nodules, where they were localized in the nitrogen-fixing zone III. In the Vicia faba system, we isolated the promoter of two alleles of the VfNOD28/32 gene by PCR-based DNA walking. The promoter sequences were similar to the promoter of the M. sativa nodulin-25 gene and contained an organ-specific element at position - 68/ - 55 from the transciptional start. An analysis of transgenic V. hirsuta root nodules expressing the gus Aint reporter gene under the control of the two VfNOD28/32 promoters demonstrated that both promoters were active in the central region of transgenic V. hirsuta nodules. |
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Thesagro: |
Alfafa; Feijão Fava; Medicago Sativa; Vicia Faba. |
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Thesaurus Nal: |
Vicia hirsuta. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01912naa a2200205 a 4500
001 1621191
005 2001-01-18
008 1999 bl --- 0-- u #d
100 1 $aKUSTER, H.
245 $aAnalysis of genes encoding modular nodulins from Vicia hirsuta and Vicia faba.
260 $c1999
520 $aThe expession of genes encoding modular nodulins was analysed in the Vicia hirsuta and the Vicia faba system. From V. hirsuta root nodules, we isolated two families of transcript sequences designated VhNOD28/32-A and VhNOD28/32-B encoding nodulins homologous to the V. faba nodulins Nvf-28/32 and the Medicago sativa nodulin-25. The modular proteins encoded by the V. hirsuta transcript sequences consisted of N- and C-terminal unique modules flanking two types of repetitive modules. Specific repetitive modules were deleted from individual VhNOD28/32 transcripts. Southern hybridizations indicated the presence of either a small VhNOD28/32 gene family or a single copy gene comprising at least two alleles. VhNOD28/32 transcripts were expressed specifically in root nodules, where they were localized in the nitrogen-fixing zone III. In the Vicia faba system, we isolated the promoter of two alleles of the VfNOD28/32 gene by PCR-based DNA walking. The promoter sequences were similar to the promoter of the M. sativa nodulin-25 gene and contained an organ-specific element at position - 68/ - 55 from the transciptional start. An analysis of transgenic V. hirsuta root nodules expressing the gus Aint reporter gene under the control of the two VfNOD28/32 promoters demonstrated that both promoters were active in the central region of transgenic V. hirsuta nodules.
650 $aVicia hirsuta
650 $aAlfafa
650 $aFeijão Fava
650 $aMedicago Sativa
650 $aVicia Faba
700 1 $aPUHLER, A.
700 1 $aPERLICK, A. M.
773 $tPlant Science, Ireland$gv. 149, p. 1-11, 1999.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agropecuária Oeste. Para informações adicionais entre em contato com cpao.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
26/10/2005 |
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Data da última atualização: |
15/09/2008 |
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Autoria: |
GODOY, K. B.; ÁVILA. C. J.; PORTELA, A. C. V.; ARCE, A. C. |
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Afiliação: |
Embrapa Agropecuária Oeste, Dourados, MS. |
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Título: |
Levantamento de parasitóides de ovos de Euschistus heros na cultura da soja, em três municípios de Mato Grosso do Sul: safra 2004/2005. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. |
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Páginas: |
p. 119-120. |
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Série: |
(Embrapa Soja. Documentos, 257). |
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Idioma: |
Português |
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Notas: |
Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.
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Thesagro: |
Entomologia; Pesquisa; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00820naa a2200217 a 4500
001 1251050
005 2008-09-15
008 2005 bl uuuu u00u1 u #d
100 1 $aGODOY, K. B.
245 $aLevantamento de parasitóides de ovos de Euschistus heros na cultura da soja, em três municípios de Mato Grosso do Sul$bsafra 2004/2005.
260 $c2005
300 $ap. 119-120.
490 $a(Embrapa Soja. Documentos, 257).
500 $aOrganizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.
650 $aEntomologia
650 $aPesquisa
650 $aSoja
700 1 $aÁVILA. C. J.
700 1 $aPORTELA, A. C. V.
700 1 $aARCE, A. C.
773 $tIn: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005.
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