Data/hora: 22/06/2024 08:56:39

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	18/01/2001	
Data da última atualização: 	18/01/2001	
Autoria: 	KUSTER, H.; PUHLER, A.; PERLICK, A. M.	
Título: 	Analysis of genes encoding modular nodulins from Vicia hirsuta and Vicia faba.	
Ano de publicação: 	1999	
Fonte/Imprenta: 	Plant Science, Ireland, v. 149, p. 1-11, 1999.	
Idioma: 	Inglês	
Conteúdo: 	The expession of genes encoding modular nodulins was analysed in the Vicia hirsuta and the Vicia faba system. From V. hirsuta root nodules, we isolated two families of transcript sequences designated VhNOD28/32-A and VhNOD28/32-B encoding nodulins homologous to the V. faba nodulins Nvf-28/32 and the Medicago sativa nodulin-25. The modular proteins encoded by the V. hirsuta transcript sequences consisted of N- and C-terminal unique modules flanking two types of repetitive modules. Specific repetitive modules were deleted from individual VhNOD28/32 transcripts. Southern hybridizations indicated the presence of either a small VhNOD28/32 gene family or a single copy gene comprising at least two alleles. VhNOD28/32 transcripts were expressed specifically in root nodules, where they were localized in the nitrogen-fixing zone III. In the Vicia faba system, we isolated the promoter of two alleles of the VfNOD28/32 gene by PCR-based DNA walking. The promoter sequences were similar to the promoter of the M. sativa nodulin-25 gene and contained an organ-specific element at position - 68/ - 55 from the transciptional start. An analysis of transgenic V. hirsuta root nodules expressing the gus Aint reporter gene under the control of the two VfNOD28/32 promoters demonstrated that both promoters were active in the central region of transgenic V. hirsuta nodules. 	
Thesagro: 	Alfafa; Feijão Fava; Medicago Sativa; Vicia Faba.	
Thesaurus Nal: 	Vicia hirsuta.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (24578-1) - ADD - SP - -- -  -  - 22386 - DIS