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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/06/2010 |
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Data da última atualização: |
02/09/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. B.; NASCIMENTO, L. B.; JUNIOR, M. L.; PETROFEZA, S. |
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Afiliação: |
M. B. OLIVEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; L. B. NASCIMENTO, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF; S. PETROFEZA, UNIVERSIDADE FEDERAL DE GOIÁS. |
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Título: |
Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 9, n. 2, p. 994-1004, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins. MenosPolygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be... Mostrar Tudo |
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Palavras-Chave: |
Endopolygalacturonase; Polygalacturonase-inhibiting protein. |
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Thesagro: |
Doença fúngica; Feijão; Mofo branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/18863/1/gmr776.pdf
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Marc: |
LEADER 02653naa a2200241 a 4500
001 1855158
005 2010-09-02
008 2010 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, M. B.
245 $aCharacterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.$h[electronic resource]
260 $c2010
520 $aPolygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins.
650 $aDoença fúngica
650 $aFeijão
650 $aMofo branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aEndopolygalacturonase
653 $aPolygalacturonase-inhibiting protein
700 1 $aNASCIMENTO, L. B.
700 1 $aJUNIOR, M. L.
700 1 $aPETROFEZA, S.
773 $tGenetics and Molecular Research$gv. 9, n. 2, p. 994-1004, 2010.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 95 | |
| 2. |  | SILVA, L. L. D.; BARBOSA, E. T.; JUNIOR, M. L. Densidade populacional de patógenos e antagonistas habitantes do solo em regiões produtoras de feijoeiro comum. Tropical Plant Pathology, Brasília, DF, v. 38, p. 575, ago. 2013. Suplemento. ref. 191-2. Edição dos Resumos do XLVI Congresso Brasileiro de Fitopatologia, Ouro Preto, MG, ago. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 3. | | FREITAS, L. D. D.; BARBOSA, E. T.; JUNIOR, M. L. Diversidade cultural, virulência e agressividade de Rhizoctonia spp. em solos cultivados com feijoeiro comum. Tropical Plant Pathology, Brasília, DF, v. 38, p. 576, ago. 2013. Suplemento. ref. 195-1. Edição dos Resumos do XLVI Congresso Brasileiro de Fitopatologia, Ouro Preto, MG, ago. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | CIVARDI, E. A.; SANTOS, P. F. D.; BARBOSA, E. T.; JUNIOR, M. L. Efeitos da temperatura e do período de molhamento foliar na infecção da soja por Sclerotinia sclerotiorum. Tropical Plant Pathology, Brasília, DF, v. 38, p. 463 ago. 2013. Suplemento. ref. 222-1. Edição dos Resumos do XLVI Congresso Brasileiro de Fitopatologia, Ouro Preto, MG, ago. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 10. | | SANTANA JÚNIOR, M. L.; ELER, J. P.; CARDOSO, F. F.; FERRAS, J. B. S. Interação genótipo x ambiente para ganho de peso pós-desmama de bovinos de corte compostos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 13. | | SALDANHA, E. C. M.; SILVA JÚNIOR, M. L. da; OKUMURA, R. S.; WADT, P. G. S. Normas dris para a cultura do coqueiro híbrido no estado do Pará. Revista Caatinga, Mossoró, v. 28, n. 4, p. 99 ? 109, out. ? dez., 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Rondônia. |
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| 15. | | BARBOSA, E. T.; SILVA, L. L. D.; YOSHIDA, F.; JUNIOR, M. L. Utilização de diferentes fontes de carbono por isolados de Fusarium solani. Tropical Plant Pathology, Brasília, DF, v. 38, p. 692, ago. 2013. Suplemento. ref. 720-1. Edição dos Resumos do XLVI Congresso Brasileiro de Fitopatologia, Ouro Preto, MG, ago. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 16. | | SENA, W. de L.; SILVA, G. R. da; SILVA JÚNIOR, M. L. da; FRAZÃO, D. A. C. Atributos químicos de um Latossolo amarelo, sob diferentes sistemas de uso, no nordeste do Pará. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira: anais. Porto Alegre: SBCS, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | SENA, W. de L.; SILVA, G. R. da; SILVA JÚNIOR, M. L. da; FRAZÃO, D. A. C. Atributos químicos de um latossolo amarelo, sob diferentes sistemas de uso, no nordeste do Pará. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira: livro de resumos. Gramado: UFRGS: SBCS, 2007. p. 270. 123-6821.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | SILVA JUNIOR, M. L. da; SILVA, G. R. da; LIMA JUNIOR, E. C. de; VIÉGAS, I. de J. M. Absorção de macronutrientes pelo mogno (Swietenia macrophylla King) em função da calagem. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82). FERTBIO 2006.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SANTANA JÚNIOR, M. L.; PEDROSA, V. B.; GROENEVELD, E.; FERRAZ, J. B. S.; CARDOSO, F. F.; ELER, J. P. Genotype x environment interaction for growth and reproduction traits of composite beef cattle in Brazil. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 9., 2010, Leipzig. Proceedings... Leipzig: German Society of Animal Science, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 20. | | SANTANA JUNIOR, M. L.; ELER, J. P.; CARDOSO, F. F.; ALBUQUERQUE, L. G.; BIGNARDI, A. B.; FERRAZ, J. B. S. Genotype by environment interaction for birth and weaning weights of composite beef cattle in different regions of Brazil Livestock Production Science, Amsterdam, v. 149, n. 3, p. 242-249, Nov. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 95 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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