Data/hora: 13/06/2024 02:20:22

Biblioteca(s): 	Embrapa Meio Ambiente.	
Data corrente: 	05/02/2015	
Data da última atualização: 	01/03/2023	
Autoria: 	CLEMENTE, Z.; CASTRO, V. L. S. S. de; FEITOSA, L. O.; LIMA, R.; JONSSON, C. M.; MAIA, A. de H. N.; FRACETO, L. F.	
Afiliação: 	ZAIRA CLEMENTE, IB-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; LEANDRO OLIVEIRA FEITOSA, UFSCar; RENATA DE LIMA, Universidade de Sorocaba; CLAUDIO MARTIN JONSSON, CNPMA; ALINE DE HOLANDA NUNES MAIA, CNPMA; LEONARDO FERNANDES FRACETO, UNESP-Sorocaba.	
Título: 	Biomarker evaluation in fish after prolonged exposure to nano-TiO2: influence of illumination conditions and crystal phase.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Journal of Nanoscience and Nanotechnology, Stevenson Ranch, v. 15, n. 7., p. 5424-5433, 2015.	
Idioma: 	Inglês	
Conteúdo: 	Abstract: In this study, we evaluated the effects of prolonged exposure to two different nano-TiO2 crystal phases under different illumination conditions. Fish (Piaractus mesopotamicus) were exposed for 21 days to 100 mg/L of nano-TiO2 anatase and a mixture of anatase:rutile (80%:20%) under visible light and UV light (UVA and B, 22.47 J/cm2/h). The following oxidative stress biomarkers were monitored:concentrations of lipid hydroperoxide (LPO), carbonylated proteins (PCO), and specific activitiesof superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Otherbiomarkers as well as specific activities of acid phosphatase (AP), Na+, K+-ATPase and metallothioneinlevels (MT) were also evaluated. Moreover, micronucleus and comet assays were performed to assess genotoxicity. Our results showed low toxicity of nano-TiO2 to fish and lack of titanium accumulation in muscle tissue. However, it was observed the occurrence of sublethal effects that were influenced by nano-TiO2 crystal phase and illumination condition. Pure anatase caused more oxidative damage without co-exposure to UV, while the mixture anatase:rutile caused more sublethal effects when exposure occurred under UV. These findings show that the specific activity of CAT, GST, PCO levels and comet assay are useful as biomarkers of prolonged exposure to nano-TiO2. Overall, our study substantiates the development and implementation of nanoecotoxicological protocols.	
Palavras-Chave: 	Nanopartículas; Nanotoxicity; Titan dioxide; Ultraviolet Light.	
Thesagro: 	Pacu; Peixe de água doce; Raio ultravioleta; Substância tóxica; Toxidez.	
Thesaurus Nal: 	Biomarkers; Ecotoxicology; Nanoparticles; Oxidative Stress; Piaractus mesopotamicus; Ultraviolet radiation.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Meio Ambiente (CNPMA)	
Exemplares: 	CNPMA - (13699-1) - UPC - AP - DD -  -  -  - DIS