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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
28/08/1995 |
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Data da última atualização: |
28/08/1995 |
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Autoria: |
JOHNSON, R. R.; DEHORITY, B. A.; BENTLEY, O. G. |
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Título: |
Studies on the in vitro rumen procedure: improved inoculum preparation and the effect of voilatile eatty acids on cellulose digestion. |
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Ano de publicação: |
1958 |
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Fonte/Imprenta: |
Journal of Animal Science, v.17, n.3, p.841-850, 1958. |
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Idioma: |
Inglês |
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Conteúdo: |
An improved inoculum for in vitro rumen fermentation has been prepared by discarding the
first extraction of the rumen contents, resuspending the present pulp in buffer, and re-ex-
pressing this liquor as an inoculum source. Inoculum prepared from this liquor have higher
cellulose digestion and less variation between experiments. The activity of these cells was destroyed completely by washing twice with buffer. Aeration for 15 minutes destroyed only 40% of the activity of this inoculum while incubation at 37C for 2 hours detroyed only 23% of the activity. A mixture of acetic, propionic and butyric acids (300, 300, and 30 ,g./per 100 ml., respectively) inhibited cellulose digestion. Butyric acid alone, however, at levels
of 100 to 150 mg./100 ml. of medium possessed cellulolytic-factor activity similar to that
demonstrated by 10 to 30 mg. of valeric acid. The significance of these findings is discus-
sed. |
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Thesagro: |
Digestibilidade; Fermentação; Rúmen. |
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Thesaurus Nal: |
cellulose; digestibility; fermentation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01563naa a2200217 a 4500
001 1785895
005 1995-08-28
008 1958 bl --- 0-- u #d
100 1 $aJOHNSON, R. R.
245 $aStudies on the in vitro rumen procedure$bimproved inoculum preparation and the effect of voilatile eatty acids on cellulose digestion.
260 $c1958
520 $aAn improved inoculum for in vitro rumen fermentation has been prepared by discarding the first extraction of the rumen contents, resuspending the present pulp in buffer, and re-ex- pressing this liquor as an inoculum source. Inoculum prepared from this liquor have higher cellulose digestion and less variation between experiments. The activity of these cells was destroyed completely by washing twice with buffer. Aeration for 15 minutes destroyed only 40% of the activity of this inoculum while incubation at 37C for 2 hours detroyed only 23% of the activity. A mixture of acetic, propionic and butyric acids (300, 300, and 30 ,g./per 100 ml., respectively) inhibited cellulose digestion. Butyric acid alone, however, at levels of 100 to 150 mg./100 ml. of medium possessed cellulolytic-factor activity similar to that demonstrated by 10 to 30 mg. of valeric acid. The significance of these findings is discus- sed.
650 $acellulose
650 $adigestibility
650 $afermentation
650 $aDigestibilidade
650 $aFermentação
650 $aRúmen
700 1 $aDEHORITY, B. A.
700 1 $aBENTLEY, O. G.
773 $tJournal of Animal Science$gv.17, n.3, p.841-850, 1958.
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Registro original: |
Embrapa Pantanal (CPAP) |
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