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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/06/1997 |
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Data da última atualização: |
18/07/2023 |
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Autoria: |
JOHNSON, L. K.; MEYER, A. L.; ZINK, M. C. |
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Título: |
Detection of ovine lentivirus in seronegative sheep by in situ hybridization, PCR, and cocultivation with susceptible cells. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Clinical Immunology and Immunopathology, v. 65, n. 3, p. 254-260, Dec. 1992. |
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DOI: |
10.1016/0090-1229(92)90155-h |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Serological surveys for ovine lentivirus (OvLV), a worldwide cause of pneumonia and chronic debilitation in sheep, have demonstrated a wide range of seroprevalence rates. This study analyzed OvLV infection in a purebred sheep flock with a history of OvLV disease (flock 1), and compared the prevalence with that of a flock lacking previous OvLV-associated disease (flock 2). Serological tests (ELISA and Western blot assay) indicated that 25% of sheep of all ages in flock 1 (Group A) and 33% of animals of all ages in flock 2 (Group B) had antibodies to OvLV. In situ hybridization, however, detected viral RNA in a much larger proportion of sheep (72 and 67%, respectively). Animals less than 1 year of age rarely had antibodies to OvLV, although most harbored viral RNA. Twenty animals in this age group from flock 1 (Group C) were therefore studied more closely for infection. These yearling animals were tested serologically by ELISA and their peripheral blood-derived macrophages were cultured for 14 days to amplify any infection in these target cells. The macrophages were then tested by in situ hybridization, PCR, and cocultivation with susceptible target cells. The results of these tests showed that while only 10% of animals in Group C were seropositive, 70% were positive by in situ hybridization, PCR, and cocultivation. These data suggest that latent OvLV infection is common in sheep and that infection is frequently undetected by serological tests. |
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Palavras-Chave: |
Age Factors; Base Sequence; Cocultivacao; In situ hibridizacao; PCR; RNA viral. |
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Thesagro: |
Ovino. |
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Thesaurus Nal: |
Disease diagnosis; In situ hybridization; Lentivirus; Macrophages; Microbiology; Oligodeoxyribonucleotides; Polymerase chain reaction; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02494naa a2200337 a 4500
001 1523494
005 2023-07-18
008 1992 bl uuuu u00u1 u #d
024 7 $a10.1016/0090-1229(92)90155-h$2DOI
100 1 $aJOHNSON, L. K.
245 $aDetection of ovine lentivirus in seronegative sheep by in situ hybridization, PCR, and cocultivation with susceptible cells.$h[electronic resource]
260 $c1992
520 $aAbstract: Serological surveys for ovine lentivirus (OvLV), a worldwide cause of pneumonia and chronic debilitation in sheep, have demonstrated a wide range of seroprevalence rates. This study analyzed OvLV infection in a purebred sheep flock with a history of OvLV disease (flock 1), and compared the prevalence with that of a flock lacking previous OvLV-associated disease (flock 2). Serological tests (ELISA and Western blot assay) indicated that 25% of sheep of all ages in flock 1 (Group A) and 33% of animals of all ages in flock 2 (Group B) had antibodies to OvLV. In situ hybridization, however, detected viral RNA in a much larger proportion of sheep (72 and 67%, respectively). Animals less than 1 year of age rarely had antibodies to OvLV, although most harbored viral RNA. Twenty animals in this age group from flock 1 (Group C) were therefore studied more closely for infection. These yearling animals were tested serologically by ELISA and their peripheral blood-derived macrophages were cultured for 14 days to amplify any infection in these target cells. The macrophages were then tested by in situ hybridization, PCR, and cocultivation with susceptible target cells. The results of these tests showed that while only 10% of animals in Group C were seropositive, 70% were positive by in situ hybridization, PCR, and cocultivation. These data suggest that latent OvLV infection is common in sheep and that infection is frequently undetected by serological tests.
650 $aDisease diagnosis
650 $aIn situ hybridization
650 $aLentivirus
650 $aMacrophages
650 $aMicrobiology
650 $aOligodeoxyribonucleotides
650 $aPolymerase chain reaction
650 $aSheep diseases
650 $aOvino
653 $aAge Factors
653 $aBase Sequence
653 $aCocultivacao
653 $aIn situ hibridizacao
653 $aPCR
653 $aRNA viral
700 1 $aMEYER, A. L.
700 1 $aZINK, M. C.
773 $tClinical Immunology and Immunopathology$gv. 65, n. 3, p. 254-260, Dec. 1992.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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