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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
19/04/2010 |
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Data da última atualização: |
19/10/2010 |
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Autoria: |
TAMBARUSSI, E. V.; MELOTTO-PASSARIN, D. M.; GONZALEZ, S. G.; BRIGATI, J. B.; JESUS, F. A. de; BARBOSA, A. L.; DRESSANO, K.; CARRER, H. |
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Afiliação: |
EVANDRO VAGNER TAMBARUSSI, ESALQ/USP; DANILA MONTEWKA MELOTTO-PASSARIN, ESALQ/USP; SIMONE GUIDETTI GONZALEZ, ESALQ/USP; JOICE BISSOLOTI BRIGATI, ESALQ/USP; FREDERICO ALMEIDA DE JESUS, ESALQ/USP; ANDRÉ LUIZ BARBOSA, ESALQ/USP; KEINI DRESSANO, ESALQ/USP; HELAINE CARRER, ESALQ/USP. |
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Título: |
In silico analysis of Simple Sequence Repeats from chloroplast genomes of Solanaceae species. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 9, n. 4, p. 344-352, Dec. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
The availability of chloroplast genome (cpDNA) sequences of Atropa belladonna, Nicotiana sylvestris, N. tabacum, N. tomentosiformis, Solanum bulbocastanum, S. lycopersicum and S. tuberosum, which are Solanaceae species, allowed us to analyze the organization of cpSSRs in their genic and intergenic regions. In general, the number of cpSSRs in cpDNA ranged from 161 in S. tuberosum to 226 in N. tabacum, and the number of intergenic cpSSRs was higher than genic cpSSRs. The mononucleotide repeats were the most frequent in studied species, but we also identified di-, tri-, tetra-, penta- and hexanucleotide repeats. Multiple alignments of all cpSSRs sequences from Solanaceae species made the identification of nucleotide variability possible and the phylogeny was estimated by maximum parsimony. Our study showed that the plastome database can be exploited for phylogenetic analysis and biotechnological approaches. |
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Palavras-Chave: |
Análise in silico de microssatélites; CpSSR; Filogenética; Marcadores moleculares; Molecular marker; Nucleotide polymorphism; Phylogenetic; Plastomas; Plastome; Polimorfismos de nucleotídeos; Solanaceae species. |
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Categoria do assunto: |
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Marc: |
LEADER 01926naa a2200337 a 4500
001 1696998
005 2010-10-19
008 2009 bl uuuu u00u1 u #d
100 1 $aTAMBARUSSI, E. V.
245 $aIn silico analysis of Simple Sequence Repeats from chloroplast genomes of Solanaceae species.
260 $c2009
520 $aThe availability of chloroplast genome (cpDNA) sequences of Atropa belladonna, Nicotiana sylvestris, N. tabacum, N. tomentosiformis, Solanum bulbocastanum, S. lycopersicum and S. tuberosum, which are Solanaceae species, allowed us to analyze the organization of cpSSRs in their genic and intergenic regions. In general, the number of cpSSRs in cpDNA ranged from 161 in S. tuberosum to 226 in N. tabacum, and the number of intergenic cpSSRs was higher than genic cpSSRs. The mononucleotide repeats were the most frequent in studied species, but we also identified di-, tri-, tetra-, penta- and hexanucleotide repeats. Multiple alignments of all cpSSRs sequences from Solanaceae species made the identification of nucleotide variability possible and the phylogeny was estimated by maximum parsimony. Our study showed that the plastome database can be exploited for phylogenetic analysis and biotechnological approaches.
653 $aAnálise in silico de microssatélites
653 $aCpSSR
653 $aFilogenética
653 $aMarcadores moleculares
653 $aMolecular marker
653 $aNucleotide polymorphism
653 $aPhylogenetic
653 $aPlastomas
653 $aPlastome
653 $aPolimorfismos de nucleotídeos
653 $aSolanaceae species
700 1 $aMELOTTO-PASSARIN, D. M.
700 1 $aGONZALEZ, S. G.
700 1 $aBRIGATI, J. B.
700 1 $aJESUS, F. A. de
700 1 $aBARBOSA, A. L.
700 1 $aDRESSANO, K.
700 1 $aCARRER, H.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 9, n. 4, p. 344-352, Dec. 2009.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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