Data/hora: 22/06/2024 03:39:33

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 24/01/2017
Data da última atualização: | 04/12/2017
Autoria: | KAYA, E.; SOUZA, F. V. D.; GÖKDOGAN, E. Y.; CEYLAN, M.; JENDEREK, M. M.
Afiliação: | ERGUN KAYA, Faculty of Science, Mu?la S?tk? Koçman University; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EMEL YILMAZ GÖKDO?AN, Faculty of Science, Mu?la S?tk? Koçman University; MUAMMER CEYLAN, Faculty of Science, Mu?la S?tk? Koçman University; MARIA M. JENDEREK, USDA-ARS.
Título: | Cryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen.
Ano de publicação: | 2017
Fonte/Imprenta: | Turkish Journal of Biology, 2017
ISSN: | 1300-0152
Idioma: | Inglês
Conteúdo: | An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability ranged from 73.3% (Poncirus trifoliata Raf. × C. sinensis Osb. and Fortunella margarita (Lour.) Swingle) to 93.3% (C. jambhiri Lush.). Dehydration was beneficial for germination of seeds from all of the tested citrus species after the liquid nitrogen exposure. Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.
Thesagro: | Criopreservação.
Thesaurus Nal: | Cryopreservation.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (31639-1) - UPC - AP - DD - Publicação digital -  -  - DIS