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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
18/06/2019 |
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Data da última atualização: |
02/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VOLK, G. M.; JENDEREK, M. M.; STAATS, E.; SHEPHERD, A.; BONNART, R.; LEDO, A. da S.; AYALA SILVA. T. |
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Afiliação: |
ANA DA SILVA LEDO, CPATC. |
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Título: |
Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Hortciulturae, 1234, 2019. |
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ISSN: |
2406-6168 |
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Idioma: |
Português |
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Notas: |
Apresentado no Acta Horticulturae, 1234. DOI 10.17660/ActaHor. |
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Conteúdo: |
The USDA-ARS National Plant Germplasm System (NPGS) maintains 946 accessions of sugarcane (Saccharum spp.) in the field at the Subtropical Horticult sure Research Station in Miami, Florida. These accessions are particularly vulnerable to hurricanes, diseases and other threats. We sought to identify a method whereby clonally propagated sugarcane accessions could be successfully introduced into tissue culture, multiplied, and then cryopreserved as shoot tips for long-term preservation at he National Laboratory for Genetic Resources Preservation in For Collins, Colorado. For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water weresufficient to remove microbial contaminants during the introduction process. However, in some cases, cefotaxime was particularly effective for removing bacterial contamination. We found that antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure usine either PVS2 or PVS3 as cryoprotectants in droplet vitrification cryopreservation methods. Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure. Published methods for encapsulation dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained. Using these methods, shoot tip regrowth levels ranged from 0 to 50% after LN exposure. We conclude that the sugarcane cryopreservation methods that we tested are not yet ready for implementation in the NPGS. MenosThe USDA-ARS National Plant Germplasm System (NPGS) maintains 946 accessions of sugarcane (Saccharum spp.) in the field at the Subtropical Horticult sure Research Station in Miami, Florida. These accessions are particularly vulnerable to hurricanes, diseases and other threats. We sought to identify a method whereby clonally propagated sugarcane accessions could be successfully introduced into tissue culture, multiplied, and then cryopreserved as shoot tips for long-term preservation at he National Laboratory for Genetic Resources Preservation in For Collins, Colorado. For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water weresufficient to remove microbial contaminants during the introduction process. However, in some cases, cefotaxime was particularly effective for removing bacterial contamination. We found that antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure usine either PVS2 or PVS3 as cryoprotectants in droplet vitrification cryopreservation methods. Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure. Published methods for encapsulation dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained. Using these methods, shoot tip regrowth... Mostrar Tudo |
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Thesagro: |
Cana de Açúcar. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198664/1/SugarcaneISHS2019.pdf
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Marc: |
LEADER 02355naa a2200229 a 4500
001 2109915
005 2019-10-02
008 2019 bl uuuu u00u1 u #d
022 $a2406-6168
100 1 $aVOLK, G. M.
245 $aChallenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation.
260 $c2019
500 $aApresentado no Acta Horticulturae, 1234. DOI 10.17660/ActaHor.
520 $aThe USDA-ARS National Plant Germplasm System (NPGS) maintains 946 accessions of sugarcane (Saccharum spp.) in the field at the Subtropical Horticult sure Research Station in Miami, Florida. These accessions are particularly vulnerable to hurricanes, diseases and other threats. We sought to identify a method whereby clonally propagated sugarcane accessions could be successfully introduced into tissue culture, multiplied, and then cryopreserved as shoot tips for long-term preservation at he National Laboratory for Genetic Resources Preservation in For Collins, Colorado. For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water weresufficient to remove microbial contaminants during the introduction process. However, in some cases, cefotaxime was particularly effective for removing bacterial contamination. We found that antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure usine either PVS2 or PVS3 as cryoprotectants in droplet vitrification cryopreservation methods. Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure. Published methods for encapsulation dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained. Using these methods, shoot tip regrowth levels ranged from 0 to 50% after LN exposure. We conclude that the sugarcane cryopreservation methods that we tested are not yet ready for implementation in the NPGS.
650 $aCana de Açúcar
700 1 $aJENDEREK, M. M.
700 1 $aSTAATS, E.
700 1 $aSHEPHERD, A.
700 1 $aBONNART, R.
700 1 $aLEDO, A. da S.
700 1 $aAYALA SILVA. T.
773 $tActa Hortciulturae, 1234, 2019.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Unidades Centrais. |
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Data corrente: |
08/12/2020 |
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Data da última atualização: |
16/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MASCARENHAS, Y. S.; SILVA, M. A. S. da; CORRECHEL, V.; SANTOS, A. B. dos; CARVALHO, M. T. de M.; MADARI, B. E.; COELHO, A. S.; GONÇALVES, G. M. de O.; CAETANO, P. H. P. |
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Afiliação: |
YONÁ SERPA MASCARENHAS, INSTITUTO FEDERAL DO PIAUÍ; MELLISSA ANANIAS SOLER DA SILVA, CNPAF; VLÁDIA CORRECHEL, UFG; ALBERTO BAETA DOS SANTOS, CNPAF; MARCIA THAIS DE MELO CARVALHO, CNPAF; BEATA EMOKE MADARI, CNPAF; ALEXANDRE SIQUEIRA COELHO, UFG; GUSTAVO MELO de OLIVEIRA GONÇALVES, UFG; PEDRO HENRIQUE PEREIRA CAETANO, UFG. |
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Título: |
Emission of nitrous oxide in flooded rice cultivation in tropical area of Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 55, e01497, 2020. |
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ISSN: |
1678-3921 |
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DOI: |
https://doi.org/10.1590/S1678-3921.pab2020.v55.01497 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Emissão de óxido nitroso em cultivo de arroz inundado em área tropical do Brasil. |
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Conteúdo: |
Abstract - The objective of this work was to evaluate the effects of nitrogen fertilizers on the N dynamics and grain yield in flooded rice (Oryza sativa) cultivation in Brazilian tropical wetland. The experiment was carried out in a randomized complete block design with six treatments, as follows: common and protected urea; topdressing application of N doses (30, 70, and 150 kg ha-1); and one control treatment, without N fertilization. Emissions of N2O-N, global warming potential (pGWP), emission factors (EF) for mineral fertilizers, grain yield, emission intensity, nitrate, ammonium, pH, and potential redox were quantified. Gas sampling was carried out in two crop seasons of rice cultivation and in one off-season. During the flooded period of the two crop seasons, N2O fluxes did not exceed 862.41 ?g m-2 h-1 N2O-N; in the off-season, the fluxes varied from -52.95 to 274.34 ?g m-2 h-1 N2O-N. Consistent emission peaks were observed in soil draining before harvest, when the highest rate of both N sources was used, and also in the control treatment in the off-season. Protected urea does not reduce N2O emissions or EF. Nitrogen increases the grain yield. Protected urea does not have any effect on the pGWP. The concentrations of NO3 - and NH4 + in the soil are not related to N2O fluxes. Resumo - O objetivo deste trabalho foi avaliar os efeitos dos fertilizantes nitrogenados na dinâmica do N e na produtividade de arroz (Oryza sativa) inundado em áreas úmidas tropicais Brasileiras. O experimento foi realizado em delineamento de blocos ao acaso, com seis tratamentos: ureia comum e protegida; doses de N (30, 70 e 150 kg ha-1) em cobertura; e controle, sem fertilização com N. Foram quantificados emissões de N-N2O, potencial de aquecimento global (pGWP), fatores de emissão (EF) para fertilizantes minerais, rendimento de grãos, intensidade de emissão, nitrato, amônio, pH e potencial redox. As amostragens foram realizadas em duas safras e na entressafra do arroz. Durante o período de inundação das duas safras, os fluxos de N-N2O não excederam 862,41μg m2 h1; no período de entressafra, os fluxos variaram de -52,95 a 274,34 μg m-2 h-1 N-N2O. Observaram-se picos de emissão consistentes na drenagem do solo, antes da colheita, quando a dose mais alta de ambas as fontes de N foi utilizada, e também no controle na entressafra. A ureia protegida não reduz as emissões de N2O ou EF. O nitrogênio aumenta o rendimento de grãos. A ureia protegida não afeta o pGWP. As concentrações de NO3 - e NH4 + no solo não estão relacionadas aos fluxos de N2O. MenosAbstract - The objective of this work was to evaluate the effects of nitrogen fertilizers on the N dynamics and grain yield in flooded rice (Oryza sativa) cultivation in Brazilian tropical wetland. The experiment was carried out in a randomized complete block design with six treatments, as follows: common and protected urea; topdressing application of N doses (30, 70, and 150 kg ha-1); and one control treatment, without N fertilization. Emissions of N2O-N, global warming potential (pGWP), emission factors (EF) for mineral fertilizers, grain yield, emission intensity, nitrate, ammonium, pH, and potential redox were quantified. Gas sampling was carried out in two crop seasons of rice cultivation and in one off-season. During the flooded period of the two crop seasons, N2O fluxes did not exceed 862.41 ?g m-2 h-1 N2O-N; in the off-season, the fluxes varied from -52.95 to 274.34 ?g m-2 h-1 N2O-N. Consistent emission peaks were observed in soil draining before harvest, when the highest rate of both N sources was used, and also in the control treatment in the off-season. Protected urea does not reduce N2O emissions or EF. Nitrogen increases the grain yield. Protected urea does not have any effect on the pGWP. The concentrations of NO3 - and NH4 + in the soil are not related to N2O fluxes. Resumo - O objetivo deste trabalho foi avaliar os efeitos dos fertilizantes nitrogenados na dinâmica do N e na produtividade de arroz (Oryza sativa) inundado em áreas úmidas tropicais Brasileiras.... Mostrar Tudo |
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Thesagro: |
Arroz Inundado; Efeito Estufa; Fertilizante Nitrogenado; Mudança Climática; Nitrogênio; Oryza Sativa. |
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Thesaurus NAL: |
Climate change; Grain yield; Greenhouse gases; Mineral fertilizers; Nitrogen fertilizers; Nitrous oxide; Rice. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218930/1/Emission-nitrous-oxide.pdf
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Marc: |
LEADER 03896naa a2200409 a 4500
001 2127896
005 2020-12-16
008 2020 bl uuuu u00u1 u #d
022 $a1678-3921
024 7 $ahttps://doi.org/10.1590/S1678-3921.pab2020.v55.01497$2DOI
100 1 $aMASCARENHAS, Y. S.
245 $aEmission of nitrous oxide in flooded rice cultivation in tropical area of Brazil.$h[electronic resource]
260 $c2020
500 $aTítulo em português: Emissão de óxido nitroso em cultivo de arroz inundado em área tropical do Brasil.
520 $aAbstract - The objective of this work was to evaluate the effects of nitrogen fertilizers on the N dynamics and grain yield in flooded rice (Oryza sativa) cultivation in Brazilian tropical wetland. The experiment was carried out in a randomized complete block design with six treatments, as follows: common and protected urea; topdressing application of N doses (30, 70, and 150 kg ha-1); and one control treatment, without N fertilization. Emissions of N2O-N, global warming potential (pGWP), emission factors (EF) for mineral fertilizers, grain yield, emission intensity, nitrate, ammonium, pH, and potential redox were quantified. Gas sampling was carried out in two crop seasons of rice cultivation and in one off-season. During the flooded period of the two crop seasons, N2O fluxes did not exceed 862.41 ?g m-2 h-1 N2O-N; in the off-season, the fluxes varied from -52.95 to 274.34 ?g m-2 h-1 N2O-N. Consistent emission peaks were observed in soil draining before harvest, when the highest rate of both N sources was used, and also in the control treatment in the off-season. Protected urea does not reduce N2O emissions or EF. Nitrogen increases the grain yield. Protected urea does not have any effect on the pGWP. The concentrations of NO3 - and NH4 + in the soil are not related to N2O fluxes. Resumo - O objetivo deste trabalho foi avaliar os efeitos dos fertilizantes nitrogenados na dinâmica do N e na produtividade de arroz (Oryza sativa) inundado em áreas úmidas tropicais Brasileiras. O experimento foi realizado em delineamento de blocos ao acaso, com seis tratamentos: ureia comum e protegida; doses de N (30, 70 e 150 kg ha-1) em cobertura; e controle, sem fertilização com N. Foram quantificados emissões de N-N2O, potencial de aquecimento global (pGWP), fatores de emissão (EF) para fertilizantes minerais, rendimento de grãos, intensidade de emissão, nitrato, amônio, pH e potencial redox. As amostragens foram realizadas em duas safras e na entressafra do arroz. Durante o período de inundação das duas safras, os fluxos de N-N2O não excederam 862,41μg m2 h1; no período de entressafra, os fluxos variaram de -52,95 a 274,34 μg m-2 h-1 N-N2O. Observaram-se picos de emissão consistentes na drenagem do solo, antes da colheita, quando a dose mais alta de ambas as fontes de N foi utilizada, e também no controle na entressafra. A ureia protegida não reduz as emissões de N2O ou EF. O nitrogênio aumenta o rendimento de grãos. A ureia protegida não afeta o pGWP. As concentrações de NO3 - e NH4 + no solo não estão relacionadas aos fluxos de N2O.
650 $aClimate change
650 $aGrain yield
650 $aGreenhouse gases
650 $aMineral fertilizers
650 $aNitrogen fertilizers
650 $aNitrous oxide
650 $aRice
650 $aArroz Inundado
650 $aEfeito Estufa
650 $aFertilizante Nitrogenado
650 $aMudança Climática
650 $aNitrogênio
650 $aOryza Sativa
700 1 $aSILVA, M. A. S. da
700 1 $aCORRECHEL, V.
700 1 $aSANTOS, A. B. dos
700 1 $aCARVALHO, M. T. de M.
700 1 $aMADARI, B. E.
700 1 $aCOELHO, A. S.
700 1 $aGONÇALVES, G. M. de O.
700 1 $aCAETANO, P. H. P.
773 $tPesquisa Agropecuária Brasileira$gv. 55, e01497, 2020.
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Embrapa Arroz e Feijão (CNPAF) |
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