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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
21/06/2001 |
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Data da última atualização: |
21/06/2001 |
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Autoria: |
JAMES, E. K.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JÚNIOR, F. B. dos; SILVA, L. G. da; REIS, V. M. |
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Título: |
Further observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 52, n. 357, p. 747-760, apr. 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
Sugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody. MenosSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem p... Mostrar Tudo |
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Palavras-Chave: |
Bactéria endofítica; Biological nitrogen fixation; BNF; Cana-de-açúcar; Casa de vegetação; Endophytic bacteria; FBN; Fixação bológica de nitrogênio; Greenhouse; Saccharum spp. |
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Thesaurus Nal: |
Gluconacetobacter diazotrophicus; sugarcane. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03060naa a2200325 a 4500
001 1598609
005 2001-06-21
008 2001 bl --- 0-- u #d
100 1 $aJAMES, E. K.
245 $aFurther observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions.
260 $c2001
520 $aSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody.
650 $aGluconacetobacter diazotrophicus
650 $asugarcane
653 $aBactéria endofítica
653 $aBiological nitrogen fixation
653 $aBNF
653 $aCana-de-açúcar
653 $aCasa de vegetação
653 $aEndophytic bacteria
653 $aFBN
653 $aFixação bológica de nitrogênio
653 $aGreenhouse
653 $aSaccharum spp
700 1 $aOLIVARES, F. L.
700 1 $aOLIVEIRA, A. L. M. de
700 1 $aREIS JÚNIOR, F. B. dos
700 1 $aSILVA, L. G. da
700 1 $aREIS, V. M.
773 $tJournal of Experimental Botany, London$gv. 52, n. 357, p. 747-760, apr. 2001.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/04/2015 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; PÉREZ-MONTAÑO, F.; RODRÍGUEZ-CARVAJAL, M. A.; NAKATANI, A. S.; GIL-SERRANO, A.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. |
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Afiliação: |
Pablo del Cerro; AMANDA ALVES PAIVA ROLLA SANTOS, CNPSo - Pós-graduanda; DOUGLAS FABIANO GOMES, CNPSo - Pós-graduando; BETTINA BERQUÓ MARKS, CNPSo - Pós-graduanda; FRANCISCO PÉREZ-MONTAÑO; MIGUEL ÁNGEL RODRÍGUEZ-CARVAJAL; ANDRÉ SHIGUEYOSHI NAKATANI; ANTONIO GIL-SERRANO; MANUEL MEGÍAS; FRANCISCO JAVIER OLLERO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC Genomics, London, [S. l.], v. 16, n. 251, Mar. 2015. 13 p. |
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ISSN: |
1471-2164 |
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DOI: |
10.1186/s12864-015-1458-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Nodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis. MenosNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our p... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus NAL: |
Nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122208/1/regulatory-nodD1.pdf
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Marc: |
LEADER 02861naa a2200289 a 4500
001 2013361
005 2017-06-19
008 2015 bl uuuu u00u1 u #d
022 $a1471-2164
024 7 $a10.1186/s12864-015-1458-8$2DOI
100 1 $aCERRO, P. del
245 $aRegulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation.$h[electronic resource]
260 $c2015
520 $aNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aROLLA-SANTOS, A. A. P.
700 1 $aGOMES, D. F.
700 1 $aMARKS, B. B.
700 1 $aPÉREZ-MONTAÑO, F.
700 1 $aRODRÍGUEZ-CARVAJAL, M. A.
700 1 $aNAKATANI, A. S.
700 1 $aGIL-SERRANO, A.
700 1 $aMEGÍAS, M.
700 1 $aOLLERO, F. J.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics, London, [S. l.]$gv. 16, n. 251, Mar. 2015. 13 p.
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