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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/06/1995 |
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Data da última atualização: |
02/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JAMES, E. K.; REIS, V. M.; OLIVARES, F. L.; BALDANI, J. I.; DOBEREINER, Johanna. |
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Título: |
Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford, v. 45, n. 275, p. 757-766, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Significant nitrogen fixation has recently been demonstrated in Brazilian sugar cane (Saccharum officinarum) cultivars known to form associations with a number of diazotrophs, iricluding Acetobacter diazotrophicus, an acid-tolerant endophytic bacterium which grows best on a sucrose-rich medium. In a series of experiments, aseptically-grown sugar cane plantlets were rooted in a liquid medium and inocu- lated with A. diazotrophicus originally isolated from field-grown sugar cane. After 4, 7, 9, and 15 d, plaflts were examined under light, scanning and transmission electron microscopes and the presence of A. diazotrophicus on and within plant tissues was con- firmed by immunogold labelling. By 15 d, external bacterial colonization was seen on roots and lower stems, particularly at cavities in lateral root junctions. The loose cells of the root cap at root tips were a site of entry of the bacteria into root tissues. Both at lateral root junctions and root tips, bacteria were also seen in enlarged, apparently intact, epidermal cells. After 15 d, bacteria were present in xylem vessels at the base of the stem, many: -connected via mucus to spiral secondary thickening. There was no obvious patho- genic reaction to the bacteria within the xylem. From these observations, it is proposed that, under experi- mental conditions, A. diazotrophicus firstly colonized the root and lower stem epidermal surfaces and then used root tips and lateral root junctions to enter the sugar cane plant where it was distributed around the plant in the transpiration stream. It is further sug- gested that the xylem vessels in the dense shoots of mature plants are also a possible site of N2-fixation by diazotrophs as they provide the low pO2 and energy as sucrose necessary for nitrogenase activity. MenosSignificant nitrogen fixation has recently been demonstrated in Brazilian sugar cane (Saccharum officinarum) cultivars known to form associations with a number of diazotrophs, iricluding Acetobacter diazotrophicus, an acid-tolerant endophytic bacterium which grows best on a sucrose-rich medium. In a series of experiments, aseptically-grown sugar cane plantlets were rooted in a liquid medium and inocu- lated with A. diazotrophicus originally isolated from field-grown sugar cane. After 4, 7, 9, and 15 d, plaflts were examined under light, scanning and transmission electron microscopes and the presence of A. diazotrophicus on and within plant tissues was con- firmed by immunogold labelling. By 15 d, external bacterial colonization was seen on roots and lower stems, particularly at cavities in lateral root junctions. The loose cells of the root cap at root tips were a site of entry of the bacteria into root tissues. Both at lateral root junctions and root tips, bacteria were also seen in enlarged, apparently intact, epidermal cells. After 15 d, bacteria were present in xylem vessels at the base of the stem, many: -connected via mucus to spiral secondary thickening. There was no obvious patho- genic reaction to the bacteria within the xylem. From these observations, it is proposed that, under experi- mental conditions, A. diazotrophicus firstly colonized the root and lower stem epidermal surfaces and then used root tips and lateral root junctions to enter the sugar cane plant whe... Mostrar Tudo |
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Palavras-Chave: |
Acetobacter diazotrophicans; Endophyte; FBN; Fixação biológica de nitrogênio. |
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Thesagro: |
Bactéria; Cana de Açúcar; Infecção. |
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Thesaurus Nal: |
infection; nitrogen fixation; sugarcane. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02614naa a2200289 a 4500
001 1618801
005 2024-02-02
008 1994 bl uuuu u00u1 u #d
100 1 $aJAMES, E. K.
245 $aInfection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus.
260 $c1994
520 $aSignificant nitrogen fixation has recently been demonstrated in Brazilian sugar cane (Saccharum officinarum) cultivars known to form associations with a number of diazotrophs, iricluding Acetobacter diazotrophicus, an acid-tolerant endophytic bacterium which grows best on a sucrose-rich medium. In a series of experiments, aseptically-grown sugar cane plantlets were rooted in a liquid medium and inocu- lated with A. diazotrophicus originally isolated from field-grown sugar cane. After 4, 7, 9, and 15 d, plaflts were examined under light, scanning and transmission electron microscopes and the presence of A. diazotrophicus on and within plant tissues was con- firmed by immunogold labelling. By 15 d, external bacterial colonization was seen on roots and lower stems, particularly at cavities in lateral root junctions. The loose cells of the root cap at root tips were a site of entry of the bacteria into root tissues. Both at lateral root junctions and root tips, bacteria were also seen in enlarged, apparently intact, epidermal cells. After 15 d, bacteria were present in xylem vessels at the base of the stem, many: -connected via mucus to spiral secondary thickening. There was no obvious patho- genic reaction to the bacteria within the xylem. From these observations, it is proposed that, under experi- mental conditions, A. diazotrophicus firstly colonized the root and lower stem epidermal surfaces and then used root tips and lateral root junctions to enter the sugar cane plant where it was distributed around the plant in the transpiration stream. It is further sug- gested that the xylem vessels in the dense shoots of mature plants are also a possible site of N2-fixation by diazotrophs as they provide the low pO2 and energy as sucrose necessary for nitrogenase activity.
650 $ainfection
650 $anitrogen fixation
650 $asugarcane
650 $aBactéria
650 $aCana de Açúcar
650 $aInfecção
653 $aAcetobacter diazotrophicans
653 $aEndophyte
653 $aFBN
653 $aFixação biológica de nitrogênio
700 1 $aREIS, V. M.
700 1 $aOLIVARES, F. L.
700 1 $aBALDANI, J. I.
700 1 $aDOBEREINER, Johanna.
773 $tJournal of Experimental Botany, Oxford$gv. 45, n. 275, p. 757-766, 1994.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
02/07/2004 |
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Data da última atualização: |
09/05/2019 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
ANDRADE, S. R. M. de. |
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Afiliação: |
SOLANGE ROCHA MONTEIRO DE ANDRADE, CPAC. |
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Título: |
Princípios da cultura de tecidos vegetais. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Planaltina: Embrapa Cerrados, 2002. |
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Páginas: |
16 p. |
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Série: |
(Embrapa Cerrados. Documentos, 58). |
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Idioma: |
Português |
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Conteúdo: |
Aspectos gerais; Fatores que afetam a regeneração in vitro; Etapas e vias de regeneração in vitro; Principais aplicações da cultura de tecidos; Conclusão. |
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Palavras-Chave: |
Cultura vegetal; Regeneration; Rewgeneration. |
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Thesagro: |
Biotecnologia; Cultura de Tecido; Cultura In Vitro; Explante; Melhoramento Vegetal; Micropropagação; Regeneração; Tecido Vegetal. |
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Thesaurus NAL: |
biotechnology; explants; in vitro culture; micropropagation; plant breeding; tissue culture. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAC-2009/24719/1/doc_58.pdf
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Marc: |
LEADER 01050nam a2200337 a 4500
001 1546466
005 2019-05-09
008 2002 bl uuuu u0uu1 u #d
100 1 $aANDRADE, S. R. M. de
245 $aPrincípios da cultura de tecidos vegetais.
260 $aPlanaltina: Embrapa Cerrados$c2002
300 $a16 p.
490 $a(Embrapa Cerrados. Documentos, 58).
520 $aAspectos gerais; Fatores que afetam a regeneração in vitro; Etapas e vias de regeneração in vitro; Principais aplicações da cultura de tecidos; Conclusão.
650 $abiotechnology
650 $aexplants
650 $ain vitro culture
650 $amicropropagation
650 $aplant breeding
650 $atissue culture
650 $aBiotecnologia
650 $aCultura de Tecido
650 $aCultura In Vitro
650 $aExplante
650 $aMelhoramento Vegetal
650 $aMicropropagação
650 $aRegeneração
650 $aTecido Vegetal
653 $aCultura vegetal
653 $aRegeneration
653 $aRewgeneration
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