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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
05/03/2013 |
Data da última atualização: |
11/03/2013 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BRASILEIRO, A. C. M.; MORGANTE, C. V.; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; PAPPAS, G.; SILVA JUNIOR, O. B. da; MARTINS, A. C. Q.; BERTIOLI, D.; GUIMARAES, P. M. |
Afiliação: |
ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CAROLINA VIANNA MORGANTE, CPATSA; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, CENARGEN; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ABADIO HERMES VIEIRA, CPAF-RO; PATRICIA MESSEMBERG GUIMARAES, CENARGEN. |
Título: |
Transcriptome analysis in response to gradual water deficit in Arachis wild relatives. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012. |
Idioma: |
Inglês |
Conteúdo: |
Peanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization of new alleles and development of molecular markers in Arachis. MenosPeanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization ... Mostrar Tudo |
Palavras-Chave: |
Cultura resistente a seca; Drought-prone areas; Melhoramento genético; Peanut; Tolerância à seca. |
Thesagro: |
Amendoim; Arachis Hypogaea; Melhoramento genético vegetal; Planta oleaginosa. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77977/1/MORGANTE0002.pdf
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Marc: |
LEADER 02609nam a2200313 a 4500 001 1952220 005 2013-03-11 008 2012 bl uuuu u00u1 u #d 100 1 $aBRASILEIRO, A. C. M. 245 $aTranscriptome analysis in response to gradual water deficit in Arachis wild relatives.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT$c2012 520 $aPeanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization of new alleles and development of molecular markers in Arachis. 650 $aAmendoim 650 $aArachis Hypogaea 650 $aMelhoramento genético vegetal 650 $aPlanta oleaginosa 653 $aCultura resistente a seca 653 $aDrought-prone areas 653 $aMelhoramento genético 653 $aPeanut 653 $aTolerância à seca 700 1 $aMORGANTE, C. V. 700 1 $aBERTIOLI, S. C. de M. L. 700 1 $aARAUJO, A. C. G. de 700 1 $aPAPPAS, G. 700 1 $aSILVA JUNIOR, O. B. da 700 1 $aMARTINS, A. C. Q. 700 1 $aBERTIOLI, D. 700 1 $aGUIMARAES, P. M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registros recuperados : 114 | |
4. | | RESENDE, R. T.; SILVA JUNIOR, O. B.; GRATTAPAGLIA, D. ENVIROTYPING in forest tree breeding: exploitation of genotype by environment interaction to avoid misallocation of genotypes in field deployment. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 313-314. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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7. | | CARNEIRO, F. de A.; MARRACCINI, P.; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D.; ANDRADE, A. C. Development and validation of a 26K Axiom® SNP array for Coffea canephora. In: SIMPÓSIO INTERNACIONAL DE GENÉTICA E MELHORAMENTO, 8., 2017, Viçosa, MG. Ômicas: do gene ao fenótipo. [Proceedings...] Viçosa, MG: UFV, 2017.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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8. | | CARNEIRO, F. de A.; MARRACCINI, P.; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D.; ANDRADE, A. C. Development and validation of a 26K Axiom® SNP array for Coffea canephora. In: SIMPÓSIO INTERNACIONAL DE GENÉTICA E MELHORAMENTO, 8., 2017, Viçosa, MG. Ômicas: do gene ao fenótipo. [Proceedings...] Viçosa, MG: UFV, 2017.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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10. | | SILVA JUNIOR, O. B.; LIRA, M.; BUSO, G. S. C.; AMARAL, Z. P. S.; GRATTAPAGLIA, D. Descoberta e genotipagem de SNPs em cajueiro via sequencimento de representações genômicas reduzidas. In. CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014. Resumo. 658. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | SILVA-JUNIOR, O. B.; GRATTAPAGLIA, D.; NOVAES, E.; COLLEVATTI, R. G. Genome assembly of the Pink Ipê (Handroanthus impetiginosus, Bignoniaceae), a highly valued, ecologically keystone Neotropical timber forest tree. GigaScience, v. 7, p. 1-16, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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16. | | TANNO, P.; SILVA JUNIOR, O. B. da; RESENDE, L. V.; SOUSA, V. A. de; GRATTAPAGLIA, D. A genotyping array of 3,400 Single Nucleotide Polymorphisms (SNPs) advances the genetic analysis of the iconic tree Araucaria angustifolia, showing that the natural populations ar e more differentiated than previously reported. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 188. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Florestas. |
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19. | | GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da; RESENDE, L. V.; SILVA, P. I. T. A five-species 50K Axiom SNP microarray allows high quality genotyping of coffee, cashew, cassava, brazilian pine and eucalyptus. In: PLANT AND ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. [Abstracts...]. San Diego, CA: [s.n.], 2017.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 114 | |
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