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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
21/03/2019 |
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Data da última atualização: |
21/03/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. |
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Afiliação: |
MARCO AURELIO CALDAS DE PINHO PESSO, CPAC; FAUSTO DE SOUZA SOBRINHO, CNPGL; RODRIGO DA ROCHA FRAGOSO, CPAC; ORZENIL BONFIM DA SILVA JUNIOR, Cenargen; MARCIO ELIAS FERREIRA, Cenargen. |
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Título: |
A phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago, 2019 |
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Idioma: |
Inglês |
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Notas: |
Plant and Animal Genome XXVII Conference (PAG). |
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Conteúdo: |
Ruzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, anchoring of contigs in linkage maps, and Hi-C scaffolding. A high-quality, chromosome-scale genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses. MenosRuzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, ... Mostrar Tudo |
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Palavras-Chave: |
Brasil. |
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Thesagro: |
Capim Urochloa; Genoma. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/194810/1/abstract-PAG-XXVII.pdf
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Marc: |
LEADER 02499nam a2200205 a 4500
001 2107378
005 2019-03-21
008 2019 bl uuuu u00u1 u #d
100 1 $aPESSOA FILHO, M. A. C. de P.
245 $aA phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing.$h[electronic resource]
260 $aIn: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago$c2019
500 $aPlant and Animal Genome XXVII Conference (PAG).
520 $aRuzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, anchoring of contigs in linkage maps, and Hi-C scaffolding. A high-quality, chromosome-scale genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses.
650 $aCapim Urochloa
650 $aGenoma
653 $aBrasil
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aFRAGOSO, R. da R.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aFERREIRA, M. E.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Biblioteca |
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| Registros recuperados : 114 | |
| 63. |  | PASSOS, M. A. N.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; EMEDIATO, F. L.; TEIXEIRA, C. C.; MILLER, R. N. G. Identificação e caracterização de components genéticos de resistência a Mycosphaerella musicola em Musa acuminata. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 45., 2012, Manaus. Fito 2012. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. Suplemento. Resumo 470. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 64. | | RESENDE, R. T.; SILVA, P. I. T.; SILVA JUNIOR, O. B. da; FREITAS, M. L. M.; SEBBENN, A. M.; SOUSA, V. A. de; AGUIAR, A. V. de; GRATTAPAGLIA, D. Age trends in genetic parameters for growth performance across country-wide provenances of the iconic conifer tree Araucaria angustifolia show strong prospects for systematic breeding and early selection. Forest Ecology and Management, v. 501, 119671, Dec. 2021. 13 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia. |
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| 65. | | ALCÂNTARA, B. S.; MATA, L. R. da; ROCHA, F. S.; CHAVES, L.; TELES, M. P. C.; SILVA JUNIOR, J. F. da; SILVA, A. V. C. da; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Análise genética de três coleções de germoplasma de mangabeira (Hancornia speciosa Gomes) por genotipagem de SNPs com DArTSeq. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 7., 2022. Multifuncionalidade e qualidade de vida: [anais...]. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2022. Na publicação: Josué Francisco da Silva Jr., Orzenil B. Silva-Junior.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 66. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 67. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 68. | | MIDORIKAWA, G. E. O.; CORREA, C. L.; NORONHA, E. F.; FERREIRA FILHO, E. X.; TOGAWA, R. C.; COSTA, M. M. do C.; SILVA JUNIOR, O. B. da; GRYNBERG, P.; MILLER, R. N. G. Analysis of the transcriptome in Aspergillus tamarii during enzymatic degradation of sugarcane bagasse. Frontiers in Bioengineering and Biotechnology, v 6, article 123, 2018. Na publicação: Orzenil Bonfim Silva-Junior.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 69. | | FIRMINO, A. A. P.; FONSECA, F. C. A.; MACEDO, L. L. P.; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; SA, M. F. G. de. Cotton boll weevil (Anthonomus Grandis) transcriptome pyrosequencing and insect-pest control by RNAI gene silencing. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 279.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 70. | | CARNEIRO, F. de A.; AQUINO, S. O. de; MATTOS, N. G.; VALERIANO, J. C.; CARNEIRO, W. W. de J.; MARRACCINI, P.; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D.; ANDRADE, A. C. Desenvolvimento e validação de uma plataforma de genotipagem em larga escala para Coffea canéfora. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 71. | | PETROLI, C. D.; SANSALONI, C. P; CARLING, J.; STEANE, D. A.; VAILLANCOURT, R. E.; MYBURG, A. M.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; KILIAN, A.; GRATTAPAGLIA, D. Genomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome. PLoS ONE, v. 7, n. 9, set. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 72. | | AGUIAR, A. V. de; TEIXEIRA-FREITAS, D. M. A.; ALMEIDA FILHO, J. E. de; SOUSA, V. A. de; RESENDE, M. D. V. de; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Genomic prediction of growth traits in Pinus taeda using genome-wide sequence-based DArT-seq markers. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2015, Florence. Forests: the importance to the planet and society. [S.l.]: IBBR: ICCOM, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 73. | | AGUIAR, A. V. de; TEIXEIRA-FREITAS, D. M. A.; ALMEIDA FILHO, J. E. de; SOUSA, V. A. de; RESENDE, M. D. V. de; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Genomic prediction of growth traits in Pinus taeda using genome-wide sequence-based DArT-seq markers. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2015, Florence. Forests: the importance to the planet and society. [S.l.]: IBBR: ICCOM, 2015. Pen-drive.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 74. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & Abstract book...Hyderabad: ICRISAT, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 75. | | GUIMARAES, P. M.; MORGANTE, C. V.; ROBERTS, P. A.; SILVA JUNIOR, O. B. da; ARRAIS, L; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D.; BRASILEIRO, A. C. M. Gene expression revealed during the interaction of resistant Arachis stenosperma and Meloidogyne arenaria. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 76. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 77. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; BERTIOLI, D. Global transcriptome analysis of peanut wild species under biotic and abiotic stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 33.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 78. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 79. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; ROBERTS, P. A.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; MARTINS, A.; MORETZSOHN, M. de C.; BERTIOLI, D. Global transcriptome analysis of two peanut wild species (Arachis stenosperma and A. duranensis) under biotic and abiotic stress. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 80. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; SILVA, A. K.; MARTINS, A. C. Q.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M. Next Generation Sequencing (NGS) for discovery of droughtresponsive genes in wild Arachis. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 114 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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