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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
06/10/2021 |
Data da última atualização: |
10/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I. |
Afiliação: |
NATASHA SANT´ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JØRGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP. |
Título: |
Development of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 105, n. 20, p. 7913-7933, 2021. |
ISSN: |
1432-0614 |
DOI: |
https://doi.org/10.1007/s00253-021-11576-5 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage. MenosAbstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induc... Mostrar Tudo |
Palavras-Chave: |
Convective drying. |
Thesagro: |
Cigarrinha; Fermentação; Formulação; Fungo Entomógeno; Fungo Para Controle Biológico; Pó Molhável; Praga de Planta. |
Thesaurus Nal: |
Biological control agents; Dalbulus maidis; Entomopathogenic fungi; Liquid state fermentation; Metarhizium robertsii; Pesticide formulations; Wettable powders. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03008naa a2200373 a 4500 001 2135109 005 2022-06-10 008 2021 bl uuuu u00u1 u #d 022 $a1432-0614 024 7 $ahttps://doi.org/10.1007/s00253-021-11576-5$2DOI 100 1 $aIWANICKI, N. S. 245 $aDevelopment of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence.$h[electronic resource] 260 $c2021 520 $aAbstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage. 650 $aBiological control agents 650 $aDalbulus maidis 650 $aEntomopathogenic fungi 650 $aLiquid state fermentation 650 $aMetarhizium robertsii 650 $aPesticide formulations 650 $aWettable powders 650 $aCigarrinha 650 $aFermentação 650 $aFormulação 650 $aFungo Entomógeno 650 $aFungo Para Controle Biológico 650 $aPó Molhável 650 $aPraga de Planta 653 $aConvective drying 700 1 $aMASCARIN, G. M. 700 1 $aMORENO, S. G. 700 1 $aEILENBERG, J. 700 1 $aDELALIBERA JUNIOR, I. 773 $tApplied Microbiology and Biotechnology$gv. 105, n. 20, p. 7913-7933, 2021.
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Registros recuperados : 6 | |
5. | | IWANICKI, N. S.; DELALIBERA JÚNIOR, I.; FARIA, M. R. de; LOPES, R. B.; SANCHES, M. M.; SOUZA, M. L. de; SEIXAS, C. D. S.; SÓSA-GOMEZ, D. R. Controle de qualidade de produtos microbiológicos. In: MEYER, M. C.; BUENO, A. de F.; MAZARO, S. M.; SILVA, J. C. da (ed.). Bioinsumos na cultura da soja. Brasília, DF: Embrapa, 2022. p. 507-533.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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6. | | WYCKHUYS, K. A.; AKUTSE, K. S.; AMALIN, D. M.; ARAJ, S.-E.; BARRERA, G.; BELTRAN, M. J. B.; FEKIH, I. B.; CALATAYUD, P.; CICERO, L.; COKOLA, M. C.; COLMENAREZ, Y. C.; DESSAUVAGES, K.; DUBOIS, T.; DUROCHER-GRANGER, L.; ESPINEL, C.; FALLET, P.; FERNÁNDEZ-TRIANA, J. L.; FRANCIS, F.; GÓMEZ, J.; HADDI, K.; HARRISON, R. D.; HASEEB, M.; IWANICKI, N. S.; JABER, L. R.; KHAMIS, F. M.; LEGASPI, J. C.; LOMELI-FLORES, R. J.; LOPES, R. B.; LYU, B.; MONTOYA-LERMA, J.; MONTECALVO, M. P.; POLASZEK, A.; NGUYEN, T. D.; NURKOMAR, I.; O'HARA, J. E.; PERIER, J. D.; RAMÍREZ-ROMERO, R.; SÁNCHEZ-GARCÍA, F. J.; ROBINSON-BAKER, A. M.; SILVEIRA, L. C.; SIMEON, L.; SOLTER, L. F.; SANTOS-AMAYA, O. F.; TALAMAS, E. J.; TAVARES, W. de S.; TRABANINO, R.; TURLINGS, T. C.; VALICENTE, F. H.; VÁSQUEZ, C.; WANG, Z.; WENGRAT, A. P.; ZANG, L.-S.; ZHANG, W.; ZIMBA, K. J.; WU, K.; ELKAHKY, M.; HADI, B. A. Global scientific progress and shortfalls in biological control of the fall armyworm Spodoptera frugiperda. Biological Control, v. 191, 105460, 2024.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 6 | |
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