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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
14/07/2020 |
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Data da última atualização: |
14/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BABA, V. Y.; BRAGHINI, M. T.; SANTOS, T. B. dos; CARVALHO, K. de; SOARES, J. D. M.; IVAMOTO-SUZUKI, S. T.; MALUF, M. P.; PADILHA, L.; PACCOLA-MEIRELLES, L. D.; PEREIRA, L. F. P.; DOMINGUES, D. S. |
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Afiliação: |
VIVIANE YUMI BABA, UNIVERSIDADE ESTADUAL DE LONDRINA; MASAKO TOMA BRAGHINI, INSTITUTO AGRONÔMICO DE CAMPINAS; TIAGO BENEDITO DOS SANTOS, INSTITUTO AGRONÔMICO DO PARANÁ; KENIA DE CARVALHO, INSTITUTO AGRONÔMICO DO PARANÁ; JOÃO DANILLO MOURA SOARES, INSTITUTO AGRONÔMICO DO PARANÁ; SUZANA TIEMI IVAMOTO-SUZUKI, INSTITUTO AGRONÔMICO DO PARANÁ - UNESP; MIRIAN PEREZ MALUF, CNPCa; LILIAN PADILHA, CNPCa; LUZIA DORETTO PACCOLA-MEIRELLES, UNIVERSIDADE ESTADUAL DE LONDRINA - UNIVERSIDADE PARANAENSE; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; DOUGLAS S. DOMINGUES, INSTITUTO AGRONÔMICO DE LONDRINA. |
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Título: |
Transcriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PeerJ, v. 8, e8320, 2020. |
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DOI: |
https://doi.org/10.7717/peerj.8320 |
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Idioma: |
Inglês |
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Notas: |
eCollection 2020. |
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Conteúdo: |
This study evaluated the transcriptional profile of genes related to nitrogen (N) assimilation in coffee plants susceptible and resistant to rust fungi under N sufficiency and N suppression. For this purpose, we inoculated young coffee leaves with Hemileia vastatrix uredospores and collected them at 0, 12, 24 and 48 hours post-inoculation (HPI) to evaluate the relative expressions of genes encoding cytosolic glutamine synthetase (CaGS1 ), plastid glutamine synthetase (CaGS2 ), nitrate reductase (CaNR), and asparagine synthetase (CaAS). The genes exhibited distinct patterns of transcriptional modulation for the different genotypes and N nutritional regimes. The resistant genotype (I59) presented high levels of transcription in response to pathogen inoculation for CaNR and CaGS1 genes, evaluated under N sufficiency in the initial moments of infection (12 HPI). The gene CaGS1 also showed a peak at 48 HPI. The susceptible genotype (CV99) showed increased transcript rates of CaNR at 12 and 24 HPI in response to rust inoculation. The transcriptional patterns observed for CV99, under N suppression, were high levels for CaAS and CaGS2 at all post-inoculation times in response to coffee leaf rust disease. In addition, CaGS1 was up-regulated at 48 HPI for CV99. Cultivar I59 showed high transcript levels at 12 HPI for CaAS and peaks at 24 and 48 HPI for CaGS2 in inoculated samples. Consequently, total chlorophyl concentration was influenced by N suppression and by rust infection. Regarding enzyme activities in vitro for glutamine synthetase and CaNR, there was an increase in infected coffee leaves (I59) and under N sufficiency. Moreover, CV99 was modulated in both N nutritional regimes for GS activity in response to rust. Our results indicate that N transport genes trigger a differential modulation between genotypes through the action of rust disease. MenosThis study evaluated the transcriptional profile of genes related to nitrogen (N) assimilation in coffee plants susceptible and resistant to rust fungi under N sufficiency and N suppression. For this purpose, we inoculated young coffee leaves with Hemileia vastatrix uredospores and collected them at 0, 12, 24 and 48 hours post-inoculation (HPI) to evaluate the relative expressions of genes encoding cytosolic glutamine synthetase (CaGS1 ), plastid glutamine synthetase (CaGS2 ), nitrate reductase (CaNR), and asparagine synthetase (CaAS). The genes exhibited distinct patterns of transcriptional modulation for the different genotypes and N nutritional regimes. The resistant genotype (I59) presented high levels of transcription in response to pathogen inoculation for CaNR and CaGS1 genes, evaluated under N sufficiency in the initial moments of infection (12 HPI). The gene CaGS1 also showed a peak at 48 HPI. The susceptible genotype (CV99) showed increased transcript rates of CaNR at 12 and 24 HPI in response to rust inoculation. The transcriptional patterns observed for CV99, under N suppression, were high levels for CaAS and CaGS2 at all post-inoculation times in response to coffee leaf rust disease. In addition, CaGS1 was up-regulated at 48 HPI for CV99. Cultivar I59 showed high transcript levels at 12 HPI for CaAS and peaks at 24 and 48 HPI for CaGS2 in inoculated samples. Consequently, total chlorophyl concentration was influenced by N suppression and by rust infection. Regar... Mostrar Tudo |
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Thesagro: |
Coffea Arábica; Nitrogênio; Nutrição Vegetal; Nutriente Mineral. |
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Thesaurus Nal: |
Gene expression; Hemileia; Nitrogen fixation; Plant nutrition. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214586/1/Transcriptional-patterns-of-coffea-peerj-08-8320.pdf
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Marc: |
LEADER 02987naa a2200361 a 4500
001 2123827
005 2020-07-14
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.7717/peerj.8320$2DOI
100 1 $aBABA, V. Y.
245 $aTranscriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust.$h[electronic resource]
260 $c2020
500 $aeCollection 2020.
520 $aThis study evaluated the transcriptional profile of genes related to nitrogen (N) assimilation in coffee plants susceptible and resistant to rust fungi under N sufficiency and N suppression. For this purpose, we inoculated young coffee leaves with Hemileia vastatrix uredospores and collected them at 0, 12, 24 and 48 hours post-inoculation (HPI) to evaluate the relative expressions of genes encoding cytosolic glutamine synthetase (CaGS1 ), plastid glutamine synthetase (CaGS2 ), nitrate reductase (CaNR), and asparagine synthetase (CaAS). The genes exhibited distinct patterns of transcriptional modulation for the different genotypes and N nutritional regimes. The resistant genotype (I59) presented high levels of transcription in response to pathogen inoculation for CaNR and CaGS1 genes, evaluated under N sufficiency in the initial moments of infection (12 HPI). The gene CaGS1 also showed a peak at 48 HPI. The susceptible genotype (CV99) showed increased transcript rates of CaNR at 12 and 24 HPI in response to rust inoculation. The transcriptional patterns observed for CV99, under N suppression, were high levels for CaAS and CaGS2 at all post-inoculation times in response to coffee leaf rust disease. In addition, CaGS1 was up-regulated at 48 HPI for CV99. Cultivar I59 showed high transcript levels at 12 HPI for CaAS and peaks at 24 and 48 HPI for CaGS2 in inoculated samples. Consequently, total chlorophyl concentration was influenced by N suppression and by rust infection. Regarding enzyme activities in vitro for glutamine synthetase and CaNR, there was an increase in infected coffee leaves (I59) and under N sufficiency. Moreover, CV99 was modulated in both N nutritional regimes for GS activity in response to rust. Our results indicate that N transport genes trigger a differential modulation between genotypes through the action of rust disease.
650 $aGene expression
650 $aHemileia
650 $aNitrogen fixation
650 $aPlant nutrition
650 $aCoffea Arábica
650 $aNitrogênio
650 $aNutrição Vegetal
650 $aNutriente Mineral
700 1 $aBRAGHINI, M. T.
700 1 $aSANTOS, T. B. dos
700 1 $aCARVALHO, K. de
700 1 $aSOARES, J. D. M.
700 1 $aIVAMOTO-SUZUKI, S. T.
700 1 $aMALUF, M. P.
700 1 $aPADILHA, L.
700 1 $aPACCOLA-MEIRELLES, L. D.
700 1 $aPEREIRA, L. F. P.
700 1 $aDOMINGUES, D. S.
773 $tPeerJ$gv. 8, e8320, 2020.
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Embrapa Café (CNPCa) |
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