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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
23/01/2004 |
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Data da última atualização: |
26/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
INOUE-NAGATA, A. K.; ALBUQUERQUE, L. C.; ROCHA, W. B.; NAGATA, T. |
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Afiliação: |
ALICE KAZUKO INOUE NAGATA, CNPH; LEONARDO C. ALBUQUERQUE, UNIVERSIDADE CATÓLICA DE BRASÍLIA; WESLEY B. ROCHA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; TATSUYA NAGATA, UNIVERSIDADE CATÓLICA DE BRASÍLIA. |
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Título: |
A simple method for cloning the complete begomovirus genome using the bacteriophage o29 DNA polymerase. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 116, p. 209-211, 2004. |
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ISSN: |
1879-0984 |
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DOI: |
10.1016/j.jviromet.2003.11.015 |
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Idioma: |
Inglês |
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Conteúdo: |
The bacteriophage φDNA polymerase amplifies circular DNA in a rolling circle amplification mechanism. This characteristic was applied to amplify and clone the complete circular DNA genome of a begomovirus. Total DNA extracted from infected tissue was used as the template of an amplification reaction using the commercial kit TempliPhi (Amersham Biosciences). The amplified DNA could be used for direct sequencing and was cloned after digestion with a single cutting restriction endonuclease. The use of this enzyme simplified the cloning steps and increased the cloning efficiency of the complete genome of a circular plant DNA virus. |
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Palavras-Chave: |
Geminivírus; Polimerase. |
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Thesagro: |
Clonagem; DNA. |
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Thesaurus Nal: |
Begomovirus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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022 $a1879-0984
024 7 $a10.1016/j.jviromet.2003.11.015$2DOI
100 1 $aINOUE-NAGATA, A. K.
245 $aA simple method for cloning the complete begomovirus genome using the bacteriophage o29 DNA polymerase.$h[electronic resource]
260 $c2004
520 $aThe bacteriophage φDNA polymerase amplifies circular DNA in a rolling circle amplification mechanism. This characteristic was applied to amplify and clone the complete circular DNA genome of a begomovirus. Total DNA extracted from infected tissue was used as the template of an amplification reaction using the commercial kit TempliPhi (Amersham Biosciences). The amplified DNA could be used for direct sequencing and was cloned after digestion with a single cutting restriction endonuclease. The use of this enzyme simplified the cloning steps and increased the cloning efficiency of the complete genome of a circular plant DNA virus.
650 $aBegomovirus
650 $aClonagem
650 $aDNA
653 $aGeminivírus
653 $aPolimerase
700 1 $aALBUQUERQUE, L. C.
700 1 $aROCHA, W. B.
700 1 $aNAGATA, T.
773 $tJournal of Virological Methods$gv. 116, p. 209-211, 2004.
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Registro original: |
Embrapa Hortaliças (CNPH) |
