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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
18/12/2003 |
Data da última atualização: |
18/12/2003 |
Autoria: |
INOUE-NAGATA, A. K.; RIBEIRO, S. G.; LACORTE, C. |
Afiliação: |
Embrapa Hortaliças, Brasília, DF. |
Título: |
Infection of sweetpepper and soybean plants with a tomato begomovirus. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, v. 28, p. 393, ago. 2003. Suplemento. |
Idioma: |
Inglês |
Notas: |
Trabalho apresentado no 36º Congresso Brasileiro de Fitopatologia, 2003, Brasília. Resumo. |
Palavras-Chave: |
Bahia; Brasil; Susceptibilidade; To CMV. |
Thesagro: |
Capsicum Annuum; Glycine Max; Pimentão; Soja. |
Thesaurus Nal: |
Begomovirus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00761naa a2200253 a 4500 001 1775068 005 2003-12-18 008 2003 bl uuuu u00u1 u #d 100 1 $aINOUE-NAGATA, A. K. 245 $aInfection of sweetpepper and soybean plants with a tomato begomovirus. 260 $c2003 500 $aTrabalho apresentado no 36º Congresso Brasileiro de Fitopatologia, 2003, Brasília. Resumo. 650 $aBegomovirus 650 $aCapsicum Annuum 650 $aGlycine Max 650 $aPimentão 650 $aSoja 653 $aBahia 653 $aBrasil 653 $aSusceptibilidade 653 $aTo CMV 700 1 $aRIBEIRO, S. G. 700 1 $aLACORTE, C. 773 $tFitopatologia Brasileira, Brasília$gv. 28, p. 393, ago. 2003. Suplemento.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
14/02/2011 |
Data da última atualização: |
19/07/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
Idioma: |
Inglês |
Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
Thesaurus NAL: |
mitochondrial DNA. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
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Marc: |
LEADER 03301naa a2200313 a 4500 001 1876985 005 2022-07-19 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.rbmo.2010.10.011$2DOI 100 1 $aCHIARATTI, M. R. 245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource] 260 $c2011 520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. 650 $amitochondrial DNA 653 $aEmbryo 653 $aEthidium bromide 653 $aOocyte 653 $aOoplasm 653 $aTransfer 700 1 $aFERREIRA, C. R. 700 1 $aPERECIN, F. 700 1 $aNICIURA, S. C. M. 700 1 $aSANGALLI, J. R. 700 1 $aMESQUITA, L. G. 700 1 $aBALIEIRO, J. C. de C. 700 1 $aSMITH, L. C. 700 1 $aGARCIA, J. M. 700 1 $aMEIRELLES, F. V. 773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
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