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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/07/2012 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
YOSHIARA, L. Y.; MADEIRA, T. B.; RIBEIRO, M. L. L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; IDA, E. I. |
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Afiliação: |
L.Y. YOSHIARA, UEL; T.B. MADEIRA, UEL; M.L.L. RIBEIRO, UEL; JOSE MARCOS GONTIJO MANDARINO, CNPSO; MERCEDES CONCORDIA CARRAO PANIZZI, CNPT; ELZA IOUKO IDA, UEL. |
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Título: |
B-glucosidase activity of soybean (Glycine max) embryonic axis germinated in the presence or absence of light. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Food Biochemistry, v. 36, n. 6, p. 699-705, Dec. 2012. |
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DOI: |
doi:10.1111/j.1745-4514.2011.00585.x |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the b-glucosidase activity of radicles, hypocotyls, cotyledons and epicotyls of soybeans (Glycine max) grown in the presence and absence of light and to obtain an alternative source of b-glucosidase. Seeds were germinated in the presence and absence of light at various time points, and the radicles, epicotyls, hypocotyls and cotyledons were separated. The enzymatic activity in the epicotyls presented the highest activity at 144 h in the presence of light. Specifically, the enzymatic activity of the epicotyls was sixfold higher than that of radicles and 12-fold higher than that of cotyledons. Interestingly, the specific activity of hypocotyls and cotyledons was 4.02-fold and 5.17-fold lower than that of epicotyls after 144 h of germination under light. The high specific activity of lightgerminated epicotyls suggested that the purity of b-glucosidase from epicotyls is greater than b-glucosidase obtained fromother parts of the plant. PRACTICAL APPLICATIONS: Isoflavones are beneficial to human health. Specifically, isoflavones and estrogen have similar positive effects on the symptoms of menopause and osteoporosis. Moreover, isoflavones possess antioxidant and antitumoral properties against certain cancers. In nature, the most common form of the isoflavone is the glycosylated form. Studies on the bioavailability of isoflavones indicate that these compounds behave similarly to estrogen in most biological systems and the aglycone forms aremore bioavailable than their glycoside forms. b-glucosidase is produced from the metabolism of fungi, bacteria or soybeans, and is the only enzyme capable of hydrolyzing isoflavone glycosides to aglycones. Therefore, safe and effective methods for the synthesis of b-glucosidase must be developed to produce soy-based foods with high concentrations of aglycone isoflavones. Thus, the objective of the present study is to investigate the best conditions under which the greatest amount of b-glucosidase could be isolated from soybean. MenosThe aim of this study was to investigate the b-glucosidase activity of radicles, hypocotyls, cotyledons and epicotyls of soybeans (Glycine max) grown in the presence and absence of light and to obtain an alternative source of b-glucosidase. Seeds were germinated in the presence and absence of light at various time points, and the radicles, epicotyls, hypocotyls and cotyledons were separated. The enzymatic activity in the epicotyls presented the highest activity at 144 h in the presence of light. Specifically, the enzymatic activity of the epicotyls was sixfold higher than that of radicles and 12-fold higher than that of cotyledons. Interestingly, the specific activity of hypocotyls and cotyledons was 4.02-fold and 5.17-fold lower than that of epicotyls after 144 h of germination under light. The high specific activity of lightgerminated epicotyls suggested that the purity of b-glucosidase from epicotyls is greater than b-glucosidase obtained fromother parts of the plant. PRACTICAL APPLICATIONS: Isoflavones are beneficial to human health. Specifically, isoflavones and estrogen have similar positive effects on the symptoms of menopause and osteoporosis. Moreover, isoflavones possess antioxidant and antitumoral properties against certain cancers. In nature, the most common form of the isoflavone is the glycosylated form. Studies on the bioavailability of isoflavones indicate that these compounds behave similarly to estrogen in most biological systems and the aglycone forms arem... Mostrar Tudo |
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Palavras-Chave: |
Isoflavona. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02707naa a2200205 a 4500
001 1927991
005 2017-08-03
008 2012 bl uuuu u00u1 u #d
024 7 $adoi:10.1111/j.1745-4514.2011.00585.x$2DOI
100 1 $aYOSHIARA, L. Y.
245 $aB-glucosidase activity of soybean (Glycine max) embryonic axis germinated in the presence or absence of light.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the b-glucosidase activity of radicles, hypocotyls, cotyledons and epicotyls of soybeans (Glycine max) grown in the presence and absence of light and to obtain an alternative source of b-glucosidase. Seeds were germinated in the presence and absence of light at various time points, and the radicles, epicotyls, hypocotyls and cotyledons were separated. The enzymatic activity in the epicotyls presented the highest activity at 144 h in the presence of light. Specifically, the enzymatic activity of the epicotyls was sixfold higher than that of radicles and 12-fold higher than that of cotyledons. Interestingly, the specific activity of hypocotyls and cotyledons was 4.02-fold and 5.17-fold lower than that of epicotyls after 144 h of germination under light. The high specific activity of lightgerminated epicotyls suggested that the purity of b-glucosidase from epicotyls is greater than b-glucosidase obtained fromother parts of the plant. PRACTICAL APPLICATIONS: Isoflavones are beneficial to human health. Specifically, isoflavones and estrogen have similar positive effects on the symptoms of menopause and osteoporosis. Moreover, isoflavones possess antioxidant and antitumoral properties against certain cancers. In nature, the most common form of the isoflavone is the glycosylated form. Studies on the bioavailability of isoflavones indicate that these compounds behave similarly to estrogen in most biological systems and the aglycone forms aremore bioavailable than their glycoside forms. b-glucosidase is produced from the metabolism of fungi, bacteria or soybeans, and is the only enzyme capable of hydrolyzing isoflavone glycosides to aglycones. Therefore, safe and effective methods for the synthesis of b-glucosidase must be developed to produce soy-based foods with high concentrations of aglycone isoflavones. Thus, the objective of the present study is to investigate the best conditions under which the greatest amount of b-glucosidase could be isolated from soybean.
653 $aIsoflavona
700 1 $aMADEIRA, T. B.
700 1 $aRIBEIRO, M. L. L.
700 1 $aMANDARINO, J. M. G.
700 1 $aCARRÃO-PANIZZI, M. C.
700 1 $aIDA, E. I.
773 $tJournal of Food Biochemistry$gv. 36, n. 6, p. 699-705, Dec. 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
08/07/2005 |
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Data da última atualização: |
14/07/2008 |
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Autoria: |
AQUINO, Y. M.; VILARINHO, K. R.; SILVA, S. F.; OLIVEIRA, M. R. V. |
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Título: |
Identificação de insetos de expressão quarentenáriaparaa viticultura no Brasil. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. |
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Páginas: |
p. 164. |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CENARGEN/25254/1/tales2004.pdf
https://www.cenargen.embrapa.br/publica/talento/tales2004.pdf
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Marc: |
LEADER 00592nam a2200145 a 4500
001 1185859
005 2008-07-14
008 2004 bl uuuu u00u1 u #d
100 1 $aAQUINO, Y. M.
245 $aIdentificação de insetos de expressão quarentenáriaparaa viticultura no Brasil.
260 $aIn: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia$c2004
300 $ap. 164.
700 1 $aVILARINHO, K. R.
700 1 $aSILVA, S. F.
700 1 $aOLIVEIRA, M. R. V.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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