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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/09/2019 |
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Data da última atualização: |
28/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FAQIR, N.; MUHAMMAD, A.; ALI, G. M.; SHEHZAD, A.; ALI, K.; RAHMAN, H.; FARHATULLAH; SOUZA JUNIOR, M. T.; ALI, M.; AKHTAR, M. N.; HYDER, M. Z. |
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Afiliação: |
Nadia Faqir, PARC Institute of Advanced Studies in Agriculture, National Agricultural Research Centre, Islamabad, Pakistan.; Aish Muhammad, National Agricultural Research Centre, Islamabad, Pakistan.; Ghulam Muhammad Ali, National Agricultural Research Centre, Islamabad, Pakistan.; Armghan Shehzad, National Agricultural Research Centre, Islamabad, Pakistan.; Kazim Ali, National Agricultural Research Centre, Islamabad, Pakistan.; Hafeez Ur Rahman, Horticulture Research Institute, National Agricultural Research Centre, Islamabad, Pakistan.; Farhatullah, University of Agriculture Peshawar, Pakistan.; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; Muhammad Ali, COMSATS University Islamabad, Park Road, Islamabad, Pakistan.; Malik Nadeem Akhtar, COMSATS University Islamabad, Park Road, Islamabad, Pakistan.; Muhammad Zeeshan Hyder, COMSATS University Islamabad, Park Road, Islamabad, Pakistan. |
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Título: |
Development of single nucleotide polymorphism-based genetic identification key for date palm cultivars. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Crop Science, n. 59, p. 2509-2520, 2019. |
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DOI: |
10.2135/cropsci2019.01.0016 |
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Idioma: |
Inglês |
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Conteúdo: |
Date palm has a long history of cultivation and valuable germplasm in Pakistan with little knowledge about its genetic makeup and diversity. The objective of this work was to develop a DNA sequence based genetic identification system for date palm cultivars. DNA fragments of rbcL (~500bp), atpB (~500bp) and 16S rRNA (~1000bp) genes of chloroplast genome and twelve DNA fragments from nuclear genome of seven cultivars were sequenced. Sequence analysis indicated that three fragments had higher marker index (MI) of 4.61, 3.61 and 2.26 with eight, seven and five SNPs respectively. Eight novel SNP sites were also found in the sequenced fragments. Based on sequenced data, a SNP typing system was developed for varietal identification of date palm cultivars, using unique SNP signatures. To validate this system, four highest SNP bearing fragments were sequenced from 30 additional date palm cultivars. The SNP data from these four fragments were able to resolve 70% of the cultivars with unique signatures. This strategy could be expanded to search more SNP sites to achieve improved resolution for identification of date palm germplasm. |
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Palavras-Chave: |
DNA sequence. |
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Thesaurus Nal: |
Pakistan. |
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Categoria do assunto: |
-- |
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Marc: |
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005 2020-01-28
008 2019 bl uuuu u00u1 u #d
024 7 $a10.2135/cropsci2019.01.0016$2DOI
100 1 $aFAQIR, N.
245 $aDevelopment of single nucleotide polymorphism-based genetic identification key for date palm cultivars.$h[electronic resource]
260 $c2019
520 $aDate palm has a long history of cultivation and valuable germplasm in Pakistan with little knowledge about its genetic makeup and diversity. The objective of this work was to develop a DNA sequence based genetic identification system for date palm cultivars. DNA fragments of rbcL (~500bp), atpB (~500bp) and 16S rRNA (~1000bp) genes of chloroplast genome and twelve DNA fragments from nuclear genome of seven cultivars were sequenced. Sequence analysis indicated that three fragments had higher marker index (MI) of 4.61, 3.61 and 2.26 with eight, seven and five SNPs respectively. Eight novel SNP sites were also found in the sequenced fragments. Based on sequenced data, a SNP typing system was developed for varietal identification of date palm cultivars, using unique SNP signatures. To validate this system, four highest SNP bearing fragments were sequenced from 30 additional date palm cultivars. The SNP data from these four fragments were able to resolve 70% of the cultivars with unique signatures. This strategy could be expanded to search more SNP sites to achieve improved resolution for identification of date palm germplasm.
650 $aPakistan
653 $aDNA sequence
700 1 $aMUHAMMAD, A.
700 1 $aALI, G. M.
700 1 $aSHEHZAD, A.
700 1 $aALI, K.
700 1 $aRAHMAN, H.
700 1 $aFARHATULLAH
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aALI, M.
700 1 $aAKHTAR, M. N.
700 1 $aHYDER, M. Z.
773 $tCrop Science$gn. 59, p. 2509-2520, 2019.
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Embrapa Agroenergia (CNPAE) |
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| 1. |  | FAQIR, N.; MUHAMMAD, A.; ALI, G. M.; SHEHZAD, A.; ALI, K.; RAHMAN, H.; FARHATULLAH; SOUZA JUNIOR, M. T.; ALI, M.; AKHTAR, M. N.; HYDER, M. Z. Development of single nucleotide polymorphism-based genetic identification key for date palm cultivars. Crop Science, n. 59, p. 2509-2520, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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