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Registro Completo |
Biblioteca(s): |
Embrapa Algodão; Embrapa Arroz e Feijão. |
Data corrente: |
21/11/2011 |
Data da última atualização: |
26/05/2017 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
HOFFMANN, L. V.; BORBA, T. C. de O.; ALLEM, L. N.; BARROSO, P. A. V.; MELLO, R. N. de. |
Afiliação: |
LUCIA VIEIRA HOFFMANN, CNPA; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; LAÍSA NOGUEIRA ALLEM, CNPq; PAULO AUGUSTO VIANNA BARROSO, CNPA; RAQUEL NEVES DE MELLO, CNPAF. |
Título: |
Biodiversity measures in agriculture using DNA. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: SOFO, A. Biodiversity. Croatia: InTech, 2011. |
Páginas: |
p. 103-116. |
ISBN: |
978-953-307-715-4 |
ISSN: |
978-953-307-715-4 |
Idioma: |
Inglês |
Conteúdo: |
Introduction; From nature to agriculture: why we need diversity?; Microrganisms and pests associated to plants; Genetic markers and molecular markers; How measures are taken: a brief review on the simplest and most popular tools; Random amplified DNA reveals polymorphism; Repetitive sequences can be especially polymorphic; Sequencing and single nucleotide polymorphism; Markers may help to understand evolution; The gains in plant breeding depends of variance; Conclusion. |
Thesagro: |
Agricultura; Biodiversidade; DNA; Marcador molecular. |
Thesaurus Nal: |
Biodiversity; Genetic markers. |
Categoria do assunto: |
-- S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/50512/1/Hoffmann.pdf
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Marc: |
LEADER 01220naa a2200277 a 4500 001 1909247 005 2017-05-26 008 2011 bl uuuu u00u1 u #d 020 $a978-953-307-715-4 022 $a978-953-307-715-4 100 1 $aHOFFMANN, L. V. 245 $aBiodiversity measures in agriculture using DNA.$h[electronic resource] 260 $c2011 300 $ap. 103-116. 520 $aIntroduction; From nature to agriculture: why we need diversity?; Microrganisms and pests associated to plants; Genetic markers and molecular markers; How measures are taken: a brief review on the simplest and most popular tools; Random amplified DNA reveals polymorphism; Repetitive sequences can be especially polymorphic; Sequencing and single nucleotide polymorphism; Markers may help to understand evolution; The gains in plant breeding depends of variance; Conclusion. 650 $aBiodiversity 650 $aGenetic markers 650 $aAgricultura 650 $aBiodiversidade 650 $aDNA 650 $aMarcador molecular 700 1 $aBORBA, T. C. de O. 700 1 $aALLEM, L. N. 700 1 $aBARROSO, P. A. V. 700 1 $aMELLO, R. N. de 773 $tIn: SOFO, A. Biodiversity. Croatia: InTech, 2011.
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Embrapa Arroz e Feijão (CNPAF) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
08/02/2021 |
Data da última atualização: |
09/02/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; NAVARRO, J. S. |
Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚSA. SÁNCHEZ NAVARRO, Universidad Politécnica de Valencia. |
Título: |
Unravelling the involvement of cilevirus p32 protein in the viral transport. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Scientifc Reports, n.11, v.2943, 2021. |
Idioma: |
Inglês |
Conteúdo: |
Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. MenosCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model... Mostrar Tudo |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Thesaurus NAL: |
Citrus; Plant diseases and disorders. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02172naa a2200217 a 4500 001 2129841 005 2021-02-09 008 2021 bl uuuu u00u1 u #d 100 1 $aLEASTRO, M. O. 245 $aUnravelling the involvement of cilevirus p32 protein in the viral transport.$h[electronic resource] 260 $c2021 520 $aCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. 650 $aCitrus 650 $aPlant diseases and disorders 650 $aDoença de Planta 650 $aFruta Cítrica 700 1 $aASTUA, J. de F. 700 1 $aKITAJIMA, E. W. 700 1 $aPALLÁS, V. 700 1 $aNAVARRO, J. S. 773 $tScientifc Reports$gn.11
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