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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
23/09/2009 |
Data da última atualização: |
23/09/2009 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
ALVES, M. F.; SILVA, U. C.; FREITAS, R. B. de; HOFFMANN, L. V.; CIAMPI, A. Y.; BARROSO, P. A. V. |
Afiliação: |
Milena Ferreira Alves; Uiara Cavalcante Silva, UFRPE; Rodolfo Barbosa de Freitas, UEPB; Lúcia Vieira Hoffmann, Embrapa Algodão; Ana Yamaguishi Ciampi, Embrapa Cenargen; Paulo Augusto Vianna Barroso, Embrapa Algodão. |
Título: |
Caracterização in situ e conservação de germoplasma de Gossypium Mustelinum Miers ex. watt (malvaceae) de afluentes da bacia do rio Paraguaçu no estado da Bahia. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. |
Descrição Física: |
1 CD-ROM |
ISSN: |
2175-2311 |
Idioma: |
Português |
Palavras-Chave: |
Caracterização in situ; Conservação do algodão; Gossypium mustelinum. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00883naa a2200229 a 4500 001 1428509 005 2009-09-23 008 2009 bl uuuu u00u1 u #d 022 $a2175-2311 100 1 $aALVES, M. F. 245 $aCaracterização in situ e conservação de germoplasma de Gossypium Mustelinum Miers ex. watt (malvaceae) de afluentes da bacia do rio Paraguaçu no estado da Bahia. 260 $c2009 300 $c1 CD-ROM 653 $aCaracterização in situ 653 $aConservação do algodão 653 $aGossypium mustelinum 700 1 $aSILVA, U. C. 700 1 $aFREITAS, R. B. de 700 1 $aHOFFMANN, L. V. 700 1 $aCIAMPI, A. Y. 700 1 $aBARROSO, P. A. V. 773 $tIn: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009.
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Embrapa Algodão (CNPA) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
27/11/2023 |
Data da última atualização: |
28/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 3 |
Autoria: |
SUNIGA, P. A. P.; MANTOVANI, C.; SANTOS, M. G. dos; EGITO, A. A. do; VERBISCK, N. V.; SANTOS, L. R. dos; DÁVILA, A. M. R.; ZIMPEL, C. K.; ZERPA, M. C. S.; CHIEBAO, D. P.; GUIMARÃES, A. M. DE S.; NASSAR, A. F. DE C.; ARAUJO, F. R. |
Afiliação: |
PAULA ADAS PEREIRA SUNIGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; CYNTHIA MANTOVANI, CNPGC; MARIA GORETTI DOS SANTOS, CNPGC; ANDREA ALVES DO EGITO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC; LENITA RAMIRES DOS SANTOS, CNPGC; ALBERTO MARTÍN RIVERA DÁVILA, INSTITUTO OSWALDO CRUZ; CRISTINA KRAEMER ZIMPEL, MICHIGAN STATE UNIVERSITY; MARIA CAROLINA SISCO ZERPA, UNIVERSIDASDE DE SÃO PAULO; DANIELA PONTES CHIEBAO, ANIMAL HEALTH RESEARCH CENTER; ANA MÁRCIA DE SÁ GUIMARÃES, UNIVERSIDADE DE SÃO PAULO; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, ANIMAL HEALTH RESEARCH CENTER; FLABIO RIBEIRO DE ARAUJO, CNPGC. |
Título: |
Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Pathogens, v. 12, e1250, 2023. |
DOI: |
https://doi.org/10.3390/pathogens12101250 |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders. |
Thesagro: |
Doença Animal; Patogenicidade. |
Thesaurus NAL: |
Animal diseases; Burkholderia mallei; Genome; Glanders; Mass spectrometry; Pathogenicity. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158739/1/Glanders-diagnosis-Asymptomatic-Mare-2023.pdf
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Marc: |
LEADER 02616naa a2200373 a 4500 001 2158739 005 2023-11-28 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/pathogens12101250$2DOI 100 1 $aSUNIGA, P. A. P. 245 $aGlanders Diagnosis in an Asymptomatic Mare from Brazil$bInsights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.$h[electronic resource] 260 $c2023 520 $aABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders. 650 $aAnimal diseases 650 $aBurkholderia mallei 650 $aGenome 650 $aGlanders 650 $aMass spectrometry 650 $aPathogenicity 650 $aDoença Animal 650 $aPatogenicidade 700 1 $aMANTOVANI, C. 700 1 $aSANTOS, M. G. dos 700 1 $aEGITO, A. A. do 700 1 $aVERBISCK, N. V. 700 1 $aSANTOS, L. R. dos 700 1 $aDÁVILA, A. M. R. 700 1 $aZIMPEL, C. K. 700 1 $aZERPA, M. C. S. 700 1 $aCHIEBAO, D. P. 700 1 $aGUIMARÃES, A. M. DE S. 700 1 $aNASSAR, A. F. DE C. 700 1 $aARAUJO, F. R. 773 $tPathogens$gv. 12, e1250, 2023.
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