Data/hora: 20/06/2024 11:48:51

Biblioteca(s): | Embrapa Algodão; Embrapa Territorial.
Data corrente: | 20/07/2022
Data da última atualização: | 04/05/2023
Autoria: | HOFFMANN, L. V.; BRANQUINHO, A. A.; BARROSO, P. A. V.; VASLIN, M. F. S.
Afiliação: | LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO.
Título: | Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease.
Ano de publicação: | 2022
Fonte/Imprenta: | Frontiers in Plant Science, v. 13, article 814119, 5 p. 2022.
ISBN: | 1664-462X
DOI: | 10.3389/fpls.2022.814119
Idioma: | Inglês
Conteúdo: | The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.
Palavras-Chave: | CLRDV; Enzyme-linked immunosorbent assay (ELISA); Intermediary host.
Thesagro: | Vírus.
Thesaurus Nal: | Serology.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144832/1/6046.pdf
Registro original: | Embrapa Algodão (CNPA)

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