Data/hora: 19/06/2024 20:46:44

Biblioteca(s): | Embrapa Pantanal.
Data corrente: | 28/04/1995
Data da última atualização: | 04/04/2017
Autoria: | CORBEIL, L. B.; HODGSON, J. L.; WIDDERS, P. R.
Título: | Immunoglobulin binding by Tritrichomonas foetus.
Ano de publicação: | 1991
Fonte/Imprenta: | Journal of Clinical Microbiology, v.29, n.12, p.2710-2714, 1991.
Idioma: | Inglês
Conteúdo: | A better method for diagnosis of bovine trichomoniasis is needed because culture is slow and somewhat lacking in sensitivity. Immunodiagnosis of Tritrichomonas foetus infection usually involves detection of antigen-antibody reactions with an anti-immunoglobulin conjugate. However, nonspecific immunoglobulin (Ig) bound to the surface of T. foetus, would also be tetected by anti-Ig conjugate and thus would interfere with the specificity of the immunoassay. The goals of this study were to define the binding of bovine immunoglobulins to T. foetus. To determine whether nonimmune binding of Ig to T. foetus occurs, we immunized rabbits with organisms that had been grown in medium containing normal bovine serum and vigorously washed three times with phosphate-buffered saline. The rabbit antiserum had similar titers to T. foetus and to normal bovine serum by enzyme-linked immunosorbent assay (ELISA). Furthermore, two bovine serum proteins were immunoprecipitated by the rabbit antiserum in an immunoelectrophoretogram. One of the serum proteins had a distribution characteristics of IgG2. The rabbit antiserum was then shown to react with purified bovine IgG and IgM by ELISA. Reactivity to IgG was greater. To identify the IgG subisotypes bound and to confirm nonimmune binding of Ig, we grew T. foetus in agammaglobulinemic fetal calf serum and reacted it with IgG1, IgG2 and IgM specific for dinitrophenol (DNP) in ELISA. The binding of IgG2 was greastest that of IgG1 was next, and that of IgM was least. Little competitive inhibition by DNP was detected, indicating that binding of DNP-specific antibodies was predominantly nonimmune rather than antigen-specific Ig binding. We also demosntrated that T. foetus grew well in medium containing agammaglobulinemic fetal calf serum or serum made agammaglobulinemic by ammonium sulfate precipitations of Igs. This may overcome the problem of low specificity in diagnostic assays as a resulted of antigen with Ig bound by nonimmune mechanisms.
Palavras-Chave: | Disease; Doencas da reproducao; Trichomonas faetus.
Thesagro: | Reprodução.
Thesaurus Nal: | reproduction.
Categoria do assunto: | --
Registro original: | Embrapa Pantanal (CPAP)
Exemplares: | CPAP - (27981-1) - ADD - SP - PP - SP14261 -  - 14261 - DIS