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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/05/2000 |
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Data da última atualização: |
16/05/2000 |
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Autoria: |
TAYLOR, N. J.; MASONA, M. V.; SCHOPKE, C.; CARCAMO, R.; HO, T.; GONZALEZ, A. E.; BEACHY, R. N.; FAUQURT, C. M. |
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Afiliação: |
ILTAB/IRD-DDPSC; University of Zimbabwe, MP 167. |
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Título: |
Production of genetically transformed cassava plants containing various genes of interest |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Brasilia: Embrapa Recursos Genetico e Biotecnologia/CBN, 2000 |
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Páginas: |
p.267-276 |
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ISBN: |
85-87697-05-6 |
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Idioma: |
Inglês |
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Notas: |
In: INTERNATIONAL SCIENTIFIC MEETING ON CASSAVA BIOTECHNOLOGY, 1998. Salvador-Bahia. Proceedings... Brasilia, DF: Embrapa Recursos Geneticos e Biotecnologia/CBN, 2000. 625p. Editado por Luiz J. C. B. Carvalho, Ann Marie Thro, Alberto Duarte Vilharinhos. |
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Conteúdo: |
The production of friable embryogenic tissues is a prerequisite for the recovery of cassava plants genetically transformed by particle bombardment. Here we report the production of friable embryogenic callus from fourteen geographically diverse cultivars of cassava. Plant regeneration has been achieved from seven of these, although the frequency at which this was achieved is cultivar dependent. Genetic transformation experiments have resulted in the recovery of transgenic plants from cvs. TMS 60444, Bonoua Rouge and M. Col 1505 containing the uidA marker gene, the coat protein gene from cassava common mosaic virus and the African cassava mosaic virus replicase gene. This demonstrates that the production of transgenic cassava with a range of transgenes is becoming a routine and reliable process, and that attention can now turn to examining the phenotype of transgenic cassava plants. |
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Palavras-Chave: |
Cultura de tecidos; Plant regeneration; Regeneracao de plantas; Tissues culture; Transformacao genetica. |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
genetic transformation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02040naa a2200325 a 4500
001 1645843
005 2000-05-16
008 2000 bl uuuu u00u1 u #d
020 $a85-87697-05-6
100 1 $aTAYLOR, N. J.
245 $aProduction of genetically transformed cassava plants containing various genes of interest
260 $c2000
300 $ap.267-276
500 $aIn: INTERNATIONAL SCIENTIFIC MEETING ON CASSAVA BIOTECHNOLOGY, 1998. Salvador-Bahia. Proceedings... Brasilia, DF: Embrapa Recursos Geneticos e Biotecnologia/CBN, 2000. 625p. Editado por Luiz J. C. B. Carvalho, Ann Marie Thro, Alberto Duarte Vilharinhos.
520 $aThe production of friable embryogenic tissues is a prerequisite for the recovery of cassava plants genetically transformed by particle bombardment. Here we report the production of friable embryogenic callus from fourteen geographically diverse cultivars of cassava. Plant regeneration has been achieved from seven of these, although the frequency at which this was achieved is cultivar dependent. Genetic transformation experiments have resulted in the recovery of transgenic plants from cvs. TMS 60444, Bonoua Rouge and M. Col 1505 containing the uidA marker gene, the coat protein gene from cassava common mosaic virus and the African cassava mosaic virus replicase gene. This demonstrates that the production of transgenic cassava with a range of transgenes is becoming a routine and reliable process, and that attention can now turn to examining the phenotype of transgenic cassava plants.
650 $agenetic transformation
650 $aMandioca
653 $aCultura de tecidos
653 $aPlant regeneration
653 $aRegeneracao de plantas
653 $aTissues culture
653 $aTransformacao genetica
700 1 $aMASONA, M. V.
700 1 $aSCHOPKE, C.
700 1 $aCARCAMO, R.
700 1 $aHO, T.
700 1 $aGONZALEZ, A. E.
700 1 $aBEACHY, R. N.
700 1 $aFAUQURT, C. M.
773 $tBrasilia: Embrapa Recursos Genetico e Biotecnologia/CBN, 2000
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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