Data/hora: 23/09/2024 12:53:56

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	10/05/2000	
Data da última atualização: 	10/05/2000	
Autoria: 	SCHOPKE, C.; MASONA, M. V.; TAYLOR, N. J.; CARCAMO, R.; HO, T.; BEACHY, R. N.; FAUQUET, C. M.	
Afiliação: 	ILTAB/IRD-DDPSC.	
Título: 	Production and characterization of transgenic cassava plants expressing the coat protein gene of cassava common mosaic virus	
Ano de publicação: 	2000	
Fonte/Imprenta: 	Brasilia: Embrapa Recursos Genetico e Biotecnologia/CBN, 2000	
Páginas: 	p.236-243	
ISBN: 	85-87697-05-6	
Idioma: 	Inglês	
Notas: 	In: INTERNATIONAL SCIENTIFIC MEETING ON CASSAVA BIOTECHNOLOGY, 1998. Salvador-Bahia. Proceedings... Brasilia, DF: Embrapa Recursos Geneticos e Biotecnologia/CBN, 2000. 625p. Editado por Luiz J. C. B. Carvalho, Ann Marie Thro, Alberto Duarte Vilarinhos	
Conteúdo: 	Genetic transformation of cassava has been achieved in several laboratories during recent years. While initially marker genes were used to optimize transformation protocols, work is now focusing on the transfer of genes of interest into this crop. One of our goals is to apply the coat protein-mediated resistance strategy to produce cassava plants resistant to a virus originating from South America, cassava common mosaic virus (cCMV). Gene constructs containing the neophosphomycin transferase gene (nptII) as selectable marker and the coat protein gene of CsCMV were transferred through particle bombardment into embryogenic suspension-derived tissue of cassava cultivar TMS 60444. Bombarded tissues were selected with paromomycin, and after regeneration of selected tissue through somatic embryogenesis eventually twenty nine transgenic plant lines were established. CsCMV coat protein was detected through Western blot analysis in nine of these lines.	
Palavras-Chave: 	Cassava transgenic; Disease; Mandioca transgenico.	
Thesagro: 	Doença; Engenharia Genética; Mandioca; Vírus.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (14858-1) - ADD - PL - -- - 633.682 - I61p. - 2000.00063 - DIS