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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
14/10/2020 |
Data da última atualização: |
12/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CÔNSOLO, N. R. B.; SAMUELSSON, L. M.; BARBOSA, L. C. G. S.; MONARETTO, T.; MORAES, T. B.; BUARQUE, V. L. M.; HIGUERA-PADILLA, A. R.; COLNAGO, L. A.; SILVA, S. L.; REIS, M. M.; FONSECA, A. C.; ARAÚJO, C. S. S.; LEITE, B. G. S.; ROQUE, F. A.; ARAÚJO, L. F. |
Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
Título: |
Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Poultry Science, v. 99, n. 11, 2020. |
Páginas: |
6247-6257 |
DOI: |
https://doi.org/10.1016/j.psj.2020.06.066 |
Idioma: |
Inglês |
Conteúdo: |
Metabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry. MenosMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transvers... Mostrar Tudo |
Palavras-Chave: |
Chicken breast myopathy; NMR spectroscopy; TD-NMR relaxometry; Water mobilization. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03360naa a2200361 a 4500 001 2125497 005 2022-08-12 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.psj.2020.06.066$2DOI 100 1 $aCÔNSOLO, N. R. B. 245 $aCharacterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry$ba pilot study.$h[electronic resource] 260 $c2020 300 $a6247-6257 520 $aMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry. 653 $aChicken breast myopathy 653 $aNMR spectroscopy 653 $aTD-NMR relaxometry 653 $aWater mobilization 700 1 $aSAMUELSSON, L. M. 700 1 $aBARBOSA, L. C. G. S. 700 1 $aMONARETTO, T. 700 1 $aMORAES, T. B. 700 1 $aBUARQUE, V. L. M. 700 1 $aHIGUERA-PADILLA, A. R. 700 1 $aCOLNAGO, L. A. 700 1 $aSILVA, S. L. 700 1 $aREIS, M. M. 700 1 $aFONSECA, A. C. 700 1 $aARAÚJO, C. S. S. 700 1 $aLEITE, B. G. S. 700 1 $aROQUE, F. A. 700 1 $aARAÚJO, L. F. 773 $tPoultry Science$gv. 99, n. 11, 2020.
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Embrapa Instrumentação (CNPDIA) |
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Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
24/03/2022 |
Data da última atualização: |
23/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MIRANDA, M.; SUN, X.; MARÍN, A.; SANTOS L. C. dos; PLOTTO, A.; BAI, J.; ASSIS, O. B. G. de; FERREIRA, M. D.; BALDWIN, E. |
Afiliação: |
ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; MARCOS DAVID FERREIRA, CNPDIA. |
Título: |
Nano- and micro-sized carnauba wax emulsions-based coatings incorporated with ginger essential oil and hydroxypropyl methylcellulose on papaya: Preservation of quality and delay of post-harvest fruit decay. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Food Chemistry: X, v. 13, 100249, 2022. |
Páginas: |
13 p. |
ISSN: |
2590-1575 |
DOI: |
https://doi.org/10.1016/j.fochx.2022.100249 |
Idioma: |
Inglês |
Conteúdo: |
Carnauba wax nano and micro-sized emulsions and hydroxypropyl methylcellulose coatings, alone or combined with ginger essential oils (GEO) were applied on papayas and evaluated under several storage conditions. In a first experiment, storage parameters were: 6 days at 22 ◦C, and 9 days at 13 ◦C followed by 5 days at 22 ◦C. In a second experiment, storage was: 5 days at 22 ◦C, and 10 days at 16 ◦C followed by 3 days at 22 ◦C. Coating effects were dependent on storage conditions. While fruits were in cold storage, there were few changes; however, at 22 ◦C, the differences between coatings became more evident. Nanoemulsions maintained papaya quality during storage by retarding firmness loss, color changes, and reducing respiration rates, resulting in delayed ripening.GEO exhibited some positive effect on fungal disease control. Nanoemulsion-based coatings improved shelf lifeby reducing weight loss, color development, and slowing ripening of papaya fruit |
Palavras-Chave: |
Edible coatings; Postharvest quality. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232903/1/P-Nano-and-micro-sized-carnauba-wax-emulsions-based-coatings.pdf
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Marc: |
LEADER 01879naa a2200277 a 4500 001 2141287 005 2024-01-23 008 2022 bl uuuu u00u1 u #d 022 $a2590-1575 024 7 $ahttps://doi.org/10.1016/j.fochx.2022.100249$2DOI 100 1 $aMIRANDA, M. 245 $aNano- and micro-sized carnauba wax emulsions-based coatings incorporated with ginger essential oil and hydroxypropyl methylcellulose on papaya$bPreservation of quality and delay of post-harvest fruit decay.$h[electronic resource] 260 $c2022 300 $a13 p. 520 $aCarnauba wax nano and micro-sized emulsions and hydroxypropyl methylcellulose coatings, alone or combined with ginger essential oils (GEO) were applied on papayas and evaluated under several storage conditions. In a first experiment, storage parameters were: 6 days at 22 ◦C, and 9 days at 13 ◦C followed by 5 days at 22 ◦C. In a second experiment, storage was: 5 days at 22 ◦C, and 10 days at 16 ◦C followed by 3 days at 22 ◦C. Coating effects were dependent on storage conditions. While fruits were in cold storage, there were few changes; however, at 22 ◦C, the differences between coatings became more evident. Nanoemulsions maintained papaya quality during storage by retarding firmness loss, color changes, and reducing respiration rates, resulting in delayed ripening.GEO exhibited some positive effect on fungal disease control. Nanoemulsion-based coatings improved shelf lifeby reducing weight loss, color development, and slowing ripening of papaya fruit 653 $aEdible coatings 653 $aPostharvest quality 700 1 $aSUN, X. 700 1 $aMARÍN, A. 700 1 $aSANTOS L. C. dos 700 1 $aPLOTTO, A. 700 1 $aBAI, J. 700 1 $aASSIS, O. B. G. de 700 1 $aFERREIRA, M. D. 700 1 $aBALDWIN, E. 773 $tFood Chemistry: X$gv. 13, 100249, 2022.
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