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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/10/1995 |
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Data da última atualização: |
10/10/1995 |
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Autoria: |
HIEBERT, E.; McDONALD, J. G. |
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Afiliação: |
Plant Pathology Department, University of Florida, Gainesuille, Florida. |
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Título: |
Capsid protein heterogeneity in turnip mosaic virus. |
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Ano de publicação: |
1976 |
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Fonte/Imprenta: |
Virology, v.70, p.144-150, 1976. |
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Idioma: |
Inglês |
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Conteúdo: |
The effect of capsid protein heterogeneity on various virus properties in purified turnip mosaic virus was investigated. Polyacrylamide-gel electrophoresis in the presence of sodium dodecyl (SDS) revealed three distinct capsid protein species, designated as fast form (MW 27,000), intermediate form (MW 29,000), slow form (MW 36,000). Virus with capsid protein in the fast or intermediate form sedimented about 12.3 S slower and had a buoyant density in CsCl about 0.01 g/cm3 greater than slow-form capsid containing virus. Immunodiffusion tests in the presence of SDS revealed that slow-form capsid protein was serologically distinct from fast or intermediate capsid forms. Little effect was detected in ultraviolet-absorption spectra virus containing either of the three capsid forms. |
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Palavras-Chave: |
Reacao serologica. |
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Thesagro: |
Proteína. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01184naa a2200157 a 4500
001 1647374
005 1995-10-10
008 1976 bl --- 0-- u #d
100 1 $aHIEBERT, E.
245 $aCapsid protein heterogeneity in turnip mosaic virus.
260 $c1976
520 $aThe effect of capsid protein heterogeneity on various virus properties in purified turnip mosaic virus was investigated. Polyacrylamide-gel electrophoresis in the presence of sodium dodecyl (SDS) revealed three distinct capsid protein species, designated as fast form (MW 27,000), intermediate form (MW 29,000), slow form (MW 36,000). Virus with capsid protein in the fast or intermediate form sedimented about 12.3 S slower and had a buoyant density in CsCl about 0.01 g/cm3 greater than slow-form capsid containing virus. Immunodiffusion tests in the presence of SDS revealed that slow-form capsid protein was serologically distinct from fast or intermediate capsid forms. Little effect was detected in ultraviolet-absorption spectra virus containing either of the three capsid forms.
650 $aProteína
653 $aReacao serologica
700 1 $aMcDONALD, J. G.
773 $tVirology$gv.70, p.144-150, 1976.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 2 | |
| 1. |  | GILBERTSON, R. L.; FARIA, J. C.; HIEBERT, E.; MAXWELL, D. P. Properties and cytopathology of bean golden mosaic from Brazil. Phytopathology, v. 78, n. 12, p. 1567-1568, Dec. 1988. Resumo 441. Resumos do Annual Meeting of the American Phytopathological Society and the Pacific Divison, San Diego, California, Nov. 1988.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 2. | | FREITAS-ASTUA, J.; ASTUA-MONGE, G.; POLSTON, J. E.; HIEBERT, E. A simple and reliable method for the screening of transgenic tobacco plants. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 7, p. 893-896, jul. 2003 Notas Científicas.
Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 2 | |
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