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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
02/03/2021 |
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Data da última atualização: |
09/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LANDIM, A. P. M.; HIDALGO CHÁVEZ, D. W.; ROSA, J. S. da; SILVA, C. M.; ROSENTHAL, A. |
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Afiliação: |
ANA PAULA MIGUEL LANDIM, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; DAVY WILLIAM HIDALGO CHÁVEZ, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; JEANE SANTOS DA ROSA, CTAA; CAROLINE MELLINGER SILVA, CTAA; AMAURI ROSENTHAL, CTAA. |
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Título: |
Effect of high hydrostatic pressure on the antioxidant capacity and peptic hydrolysis of whey proteins. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Ciência Rural, v. 51, n. 4, Santa Maria, 2021. p. 1-12. |
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DOI: |
https://doi.org/10.1590/0103-8478cr20200560 |
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Idioma: |
Inglês |
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Notas: |
Efeito da alta pressão hidrostática na hidrólise péptica das proteínas do soro de leite e na capacidade antioxidante. |
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Conteúdo: |
The effect of high hydrostatic pressure (HHP) application on whey protein concentrate was evaluated both before (pre-treatment - PT) and during (hydrolysis assisted - HA) hydrolysis processes. A factorial design 22 with 3 central points was used with pressure (100, 250, 400 MPa) and time (5, 20 and 35 minutes) as independent variables. The hydrolysis was evaluated and monitored by soluble protein, aromatic amino acid contents and RP-HPLC. ABTS and ORAC tests were used to evaluate the in vitro antioxidant capacity. The reduction of soluble protein content was approximately 20% for conventional hydrolysis and for all PT treatments up to 4 h of reaction, while HHP assisted hydrolysis at 100 MPa showed a 35% protein reduction after 35 minutes of reaction. In addition, pressurization favored peptic hydrolysis of ?-lactoglobulin by up to 98% and also improved the in vitro antioxidant capacity of the hydrolysates, which increased from 34.25 to 60.89 ?moles TE g-1 of protein in the best treatment. The results suggest that the use of HHP assisted hydrolysis favored the peptic hydrolysis, with a reduction in hydrolysis time and increased antioxidant activity. Neste estudo, o efeito da aplicação de alta pressão hidrostática (HHP) sobre o concentrado proteico de soro de leite foi avaliado antes (pré-tratamento - PT) e durante os processos de hidrólise (assistida por hidrólise - HA). Utilizou-se o delineamento fatorial 22 com três pontos centrais, onde as variáveis independentes foram pressão (100, 250, 400 MPa) e tempo (5, 20 e 35 minutos). A hidrólise foi avaliada pelo conteúdo de proteínas solúveis e aminoácidos aromáticos, além do perfil peptídico por RP-HPLC. As análises de ABTS e ORAC foram utilizadas para avaliar a capacidade antioxidante in vitro. A redução do teor de proteína solúvel foi de aproximadamente 20% para a hidrólise convencional e para todos os pontos de PT até 4h de reação, enquanto a hidrólise assistida por HHP a 100 MPa mostrou uma redução de 35% de proteína em 35 minutos de reação. Além disso, a pressurização favoreceu a hidrólise péptica da ?-lactoglobulina em até 98% e também melhorou a capacidade antioxidante in vitro dos hidrolisados, que aumentaram de 34,25 para 60,89 ?moles de TE g-1 de proteína no melhor tratamento. Os resultados sugerem que o uso da hidrólise assistida por HHP favoreceu a hidrólise péptica, com redução no tempo de hidrólise e aumento da atividade antioxidante. MenosThe effect of high hydrostatic pressure (HHP) application on whey protein concentrate was evaluated both before (pre-treatment - PT) and during (hydrolysis assisted - HA) hydrolysis processes. A factorial design 22 with 3 central points was used with pressure (100, 250, 400 MPa) and time (5, 20 and 35 minutes) as independent variables. The hydrolysis was evaluated and monitored by soluble protein, aromatic amino acid contents and RP-HPLC. ABTS and ORAC tests were used to evaluate the in vitro antioxidant capacity. The reduction of soluble protein content was approximately 20% for conventional hydrolysis and for all PT treatments up to 4 h of reaction, while HHP assisted hydrolysis at 100 MPa showed a 35% protein reduction after 35 minutes of reaction. In addition, pressurization favored peptic hydrolysis of ?-lactoglobulin by up to 98% and also improved the in vitro antioxidant capacity of the hydrolysates, which increased from 34.25 to 60.89 ?moles TE g-1 of protein in the best treatment. The results suggest that the use of HHP assisted hydrolysis favored the peptic hydrolysis, with a reduction in hydrolysis time and increased antioxidant activity. Neste estudo, o efeito da aplicação de alta pressão hidrostática (HHP) sobre o concentrado proteico de soro de leite foi avaliado antes (pré-tratamento - PT) e durante os processos de hidrólise (assistida por hidrólise - HA). Utilizou-se o delineamento fatorial 22 com três pontos centrais, onde as variáveis independentes foram pr... Mostrar Tudo |
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Palavras-Chave: |
Alta pressão hidrostática; Antioxidant capacity; Capacidade antioxidante; Hidrólise enzimática; High hydrostatic pressure. |
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Thesagro: |
Pepsina; Tecnologia de Alimento. |
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Thesaurus Nal: |
Enzymatic hydrolysis; Food technology; Pepsin. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03584naa a2200313 a 4500
001 2130420
005 2024-05-09
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/0103-8478cr20200560$2DOI
100 1 $aLANDIM, A. P. M.
245 $aEffect of high hydrostatic pressure on the antioxidant capacity and peptic hydrolysis of whey proteins.$h[electronic resource]
260 $c2021
500 $aEfeito da alta pressão hidrostática na hidrólise péptica das proteínas do soro de leite e na capacidade antioxidante.
520 $aThe effect of high hydrostatic pressure (HHP) application on whey protein concentrate was evaluated both before (pre-treatment - PT) and during (hydrolysis assisted - HA) hydrolysis processes. A factorial design 22 with 3 central points was used with pressure (100, 250, 400 MPa) and time (5, 20 and 35 minutes) as independent variables. The hydrolysis was evaluated and monitored by soluble protein, aromatic amino acid contents and RP-HPLC. ABTS and ORAC tests were used to evaluate the in vitro antioxidant capacity. The reduction of soluble protein content was approximately 20% for conventional hydrolysis and for all PT treatments up to 4 h of reaction, while HHP assisted hydrolysis at 100 MPa showed a 35% protein reduction after 35 minutes of reaction. In addition, pressurization favored peptic hydrolysis of ?-lactoglobulin by up to 98% and also improved the in vitro antioxidant capacity of the hydrolysates, which increased from 34.25 to 60.89 ?moles TE g-1 of protein in the best treatment. The results suggest that the use of HHP assisted hydrolysis favored the peptic hydrolysis, with a reduction in hydrolysis time and increased antioxidant activity. Neste estudo, o efeito da aplicação de alta pressão hidrostática (HHP) sobre o concentrado proteico de soro de leite foi avaliado antes (pré-tratamento - PT) e durante os processos de hidrólise (assistida por hidrólise - HA). Utilizou-se o delineamento fatorial 22 com três pontos centrais, onde as variáveis independentes foram pressão (100, 250, 400 MPa) e tempo (5, 20 e 35 minutos). A hidrólise foi avaliada pelo conteúdo de proteínas solúveis e aminoácidos aromáticos, além do perfil peptídico por RP-HPLC. As análises de ABTS e ORAC foram utilizadas para avaliar a capacidade antioxidante in vitro. A redução do teor de proteína solúvel foi de aproximadamente 20% para a hidrólise convencional e para todos os pontos de PT até 4h de reação, enquanto a hidrólise assistida por HHP a 100 MPa mostrou uma redução de 35% de proteína em 35 minutos de reação. Além disso, a pressurização favoreceu a hidrólise péptica da ?-lactoglobulina em até 98% e também melhorou a capacidade antioxidante in vitro dos hidrolisados, que aumentaram de 34,25 para 60,89 ?moles de TE g-1 de proteína no melhor tratamento. Os resultados sugerem que o uso da hidrólise assistida por HHP favoreceu a hidrólise péptica, com redução no tempo de hidrólise e aumento da atividade antioxidante.
650 $aEnzymatic hydrolysis
650 $aFood technology
650 $aPepsin
650 $aPepsina
650 $aTecnologia de Alimento
653 $aAlta pressão hidrostática
653 $aAntioxidant capacity
653 $aCapacidade antioxidante
653 $aHidrólise enzimática
653 $aHigh hydrostatic pressure
700 1 $aHIDALGO CHÁVEZ, D. W.
700 1 $aROSA, J. S. da
700 1 $aSILVA, C. M.
700 1 $aROSENTHAL, A.
773 $tCiência Rural$gv. 51, n. 4, Santa Maria, 2021. p. 1-12.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/12/2023 |
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Data da última atualização: |
08/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
ADUNOLA, P.; FERRÃO, M. A. G.; FERRÃO, R. G.; FONSECA, A. F. A. da; VOLPI, P. S.; COMÉRIO, M.; VERDIN FILHO, A. C.; MUNOZ, P. R.; FERRÃO, L. F. V. |
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Afiliação: |
PAUL ADUNOLA, UNIVERSITY OF FLORIDA; MARIA AMÉLIA G FERRÃO, CNPCa; ROMÁRIO G FERRÃO, INSTITUTO CAPIXABA DE PESQUISA, ASSISTÊNCIA TÉCNICA E EXTENSÃO RURAL; AYMBIRE F A DA FONSECA, INSTITUTO CAPIXABA DE PESQUISA, ASSISTÊNCIA TÉCNICA E EXTENSÃO RURAL; PAULO S VOLPI, INSTITUTO CAPIXABA DE PESQUISA, ASSISTÊNCIA TÉCNICA E EXTENSÃO RURAL; MARCONE COMÉRIO, INSTITUTO CAPIXABA DE PESQUISA, ASSISTÊNCIA TÉCNICA E EXTENSÃO RURAL; ABRAÃO C VERDIN FILHO, INSTITUTO CAPIXABA DE PESQUISA, ASSISTÊNCIA TÉCNICA E EXTENSÃO RURAL; PATRICIO R MUNOZ, UNIVERSITY OF FLORIDA; LUÍS FELIPE V FERRÃO, UNIVERSITY OF FLORIDA. |
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Título: |
Genomic selection for genotype performance and environmental stability in Coffea canéfora. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
G3: Genes, Genomes, Genetics, v. 13, n. 6, p. 1-13, 2023. |
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DOI: |
https://doi.org/10.1093/g3journal/jkad062 |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee is one of the most important beverages and trade products in the world. Among the multiple research initiatives focused on coffee sustainability, plant breeding provides the best means to increase phenotypic performance and release cultivars that could meet market demands. Since coffee is well adapted to a diversity of tropical environments, an important question for those confronting the problem of evaluating phenotypic performance is the relevance of genotype-by-environment interaction. As a perennial crop with a long juvenile phase, coffee is subjected to significant temporal and spatial variations. Such facts not only hinder the selection of promising materials but also cause a majority of complaints among growers. In this study, we hypothesized that trait stability in coffee is genetically controlled and therefore is predictable using molecular information. To test it, we used genome-based methods to predict stability metrics computed with the primary goal of selecting coffee genotypes that combine high phenotypic performance and stability for target environments. Using 2 populations of Coffea canephora, evaluated across multiple years and locations, our contribution is 3-fold: (1) first, we demonstrated that the number of harvest evaluations may be reduced leading to accelerated implementation of molecular breeding; (2) we showed that stability metrics are predictable; and finally, (3) both stable and high-performance genotypes can be simultaneously predicted and selected. While this research was carried out on representative environments for coffee production with substantial crossover in genotypic ranking, we anticipate that genomic prediction can be an efficient tool to select coffee genotypes that combine high performance and stability across years and the target locations here evaluated. MenosCoffee is one of the most important beverages and trade products in the world. Among the multiple research initiatives focused on coffee sustainability, plant breeding provides the best means to increase phenotypic performance and release cultivars that could meet market demands. Since coffee is well adapted to a diversity of tropical environments, an important question for those confronting the problem of evaluating phenotypic performance is the relevance of genotype-by-environment interaction. As a perennial crop with a long juvenile phase, coffee is subjected to significant temporal and spatial variations. Such facts not only hinder the selection of promising materials but also cause a majority of complaints among growers. In this study, we hypothesized that trait stability in coffee is genetically controlled and therefore is predictable using molecular information. To test it, we used genome-based methods to predict stability metrics computed with the primary goal of selecting coffee genotypes that combine high phenotypic performance and stability for target environments. Using 2 populations of Coffea canephora, evaluated across multiple years and locations, our contribution is 3-fold: (1) first, we demonstrated that the number of harvest evaluations may be reduced leading to accelerated implementation of molecular breeding; (2) we showed that stability metrics are predictable; and finally, (3) both stable and high-performance genotypes can be simultaneously predicted an... Mostrar Tudo |
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Thesagro: |
Coffea Canephora. |
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Thesaurus NAL: |
Genomics; Genotype; Plant breeding; Prediction. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159395/1/Genomic-selection-for-genotype.pdf
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Marc: |
LEADER 02692naa a2200289 a 4500
001 2159395
005 2023-12-08
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1093/g3journal/jkad062$2DOI
100 1 $aADUNOLA, P.
245 $aGenomic selection for genotype performance and environmental stability in Coffea canéfora.$h[electronic resource]
260 $c2023
520 $aCoffee is one of the most important beverages and trade products in the world. Among the multiple research initiatives focused on coffee sustainability, plant breeding provides the best means to increase phenotypic performance and release cultivars that could meet market demands. Since coffee is well adapted to a diversity of tropical environments, an important question for those confronting the problem of evaluating phenotypic performance is the relevance of genotype-by-environment interaction. As a perennial crop with a long juvenile phase, coffee is subjected to significant temporal and spatial variations. Such facts not only hinder the selection of promising materials but also cause a majority of complaints among growers. In this study, we hypothesized that trait stability in coffee is genetically controlled and therefore is predictable using molecular information. To test it, we used genome-based methods to predict stability metrics computed with the primary goal of selecting coffee genotypes that combine high phenotypic performance and stability for target environments. Using 2 populations of Coffea canephora, evaluated across multiple years and locations, our contribution is 3-fold: (1) first, we demonstrated that the number of harvest evaluations may be reduced leading to accelerated implementation of molecular breeding; (2) we showed that stability metrics are predictable; and finally, (3) both stable and high-performance genotypes can be simultaneously predicted and selected. While this research was carried out on representative environments for coffee production with substantial crossover in genotypic ranking, we anticipate that genomic prediction can be an efficient tool to select coffee genotypes that combine high performance and stability across years and the target locations here evaluated.
650 $aGenomics
650 $aGenotype
650 $aPlant breeding
650 $aPrediction
650 $aCoffea Canephora
700 1 $aFERRÃO, M. A. G.
700 1 $aFERRÃO, R. G.
700 1 $aFONSECA, A. F. A. da
700 1 $aVOLPI, P. S.
700 1 $aCOMÉRIO, M.
700 1 $aVERDIN FILHO, A. C.
700 1 $aMUNOZ, P. R.
700 1 $aFERRÃO, L. F. V.
773 $tG3: Genes, Genomes, Genetics$gv. 13, n. 6, p. 1-13, 2023.
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Embrapa Café (CNPCa) |
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