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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
29/03/2016 |
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Data da última atualização: |
29/03/2016 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
HELENE, L. C. F.; DELAMUTA, J. R. M.; RIBEIRO. R. A.; ORMEÑO-ORRILLO, E.; ROGEL, M. A; MARTINEZ-ROMERO, E.; HUNGRIA, M. |
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Afiliação: |
Luisa Caroline Ferraz Helene, UEL; Jakeline Renata Marçon Delamuta, UEL; Renan Augusto Ribeiro, Conselho Nacional de Desenvolvimento Cientifico e Tecnológico; Ernesto Ormeño-Orrillo, Universidad Nacional Agraria La Molina; Marco Antonio Rogel, Centro de Ciencias Genó micas, Universidad Nacional Autónoma de México; Esperanza Martinez-Romero, Centro de Ciências Genómicas, Universidad Nacional Autónoma de México; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Bradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for green manure and environmental services. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Reading v. 65, n. 12, 2015. |
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Páginas: |
p. 4441-4438 |
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Idioma: |
Português |
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Conteúdo: |
Symbiotic nitrogen-fixing bacteria, commonly called rhizobia, are agronomically important because they can provide significant amounts of nitrogen to plants and help in recovery of impoverished soils and improvement of degraded environments. In recent years, with advances in molecular techniques, several studies have shown that these bacteria have high levels of genetic diversity, resulting in taxonomic reclassifications and descriptions of new species. However, despite the advances achieved, highly conserved 16S ribosomal genes (16S rRNA) do not elucidate differences between species of several genera, including the genus Bradyrhizobium. Other methodologies, such as multilocus sequence analysis (MLSA), have been used in such cases, with good results. In this study, three strains (SEMIAs 690T, 6387 and 6428) of the genus Bradyrhizobium, isolated from nitrogen-fixing nodules of Centrosema and Acacia species, without clear taxonomic positions, were studied. These strains differed from genetically closely related species according to the results of MLSA of four housekeeping genes (dnaK, glnII, gyrB and recA) and nucleotide identities of the concatenated genes with those of related species ranged from 87.8 % to 95.7 %, being highest with Bradyrhizobium elkanii. DNA?DNA hybridization (less than 32 % DNA relatedness) and average nucleotide identity values of the whole genomes (less than 90.5 %) indicated that these strains represented a novel species, and phenotypic traits were determined. Our data supported the description of the SEMIA strains as Bradyrhizobium viridifuturi sp. nov., and SEMIA 690T (5CNPSo 991T5C 100aT5BR 1804T5LMG 28866T), isolated from Centrosema pubescens, was chosen as type strain. MenosSymbiotic nitrogen-fixing bacteria, commonly called rhizobia, are agronomically important because they can provide significant amounts of nitrogen to plants and help in recovery of impoverished soils and improvement of degraded environments. In recent years, with advances in molecular techniques, several studies have shown that these bacteria have high levels of genetic diversity, resulting in taxonomic reclassifications and descriptions of new species. However, despite the advances achieved, highly conserved 16S ribosomal genes (16S rRNA) do not elucidate differences between species of several genera, including the genus Bradyrhizobium. Other methodologies, such as multilocus sequence analysis (MLSA), have been used in such cases, with good results. In this study, three strains (SEMIAs 690T, 6387 and 6428) of the genus Bradyrhizobium, isolated from nitrogen-fixing nodules of Centrosema and Acacia species, without clear taxonomic positions, were studied. These strains differed from genetically closely related species according to the results of MLSA of four housekeeping genes (dnaK, glnII, gyrB and recA) and nucleotide identities of the concatenated genes with those of related species ranged from 87.8 % to 95.7 %, being highest with Bradyrhizobium elkanii. DNA?DNA hybridization (less than 32 % DNA relatedness) and average nucleotide identity values of the whole genomes (less than 90.5 %) indicated that these strains represented a novel species, and phenotypic traits were det... Mostrar Tudo |
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Palavras-Chave: |
Fixação biologica de nitrogenio. |
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Categoria do assunto: |
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Marc: |
LEADER 02480naa a2200217 a 4500
001 2041960
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
100 1 $aHELENE, L. C. F.
245 $aBradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for green manure and environmental services.$h[electronic resource]
260 $c2015
300 $ap. 4441-4438
520 $aSymbiotic nitrogen-fixing bacteria, commonly called rhizobia, are agronomically important because they can provide significant amounts of nitrogen to plants and help in recovery of impoverished soils and improvement of degraded environments. In recent years, with advances in molecular techniques, several studies have shown that these bacteria have high levels of genetic diversity, resulting in taxonomic reclassifications and descriptions of new species. However, despite the advances achieved, highly conserved 16S ribosomal genes (16S rRNA) do not elucidate differences between species of several genera, including the genus Bradyrhizobium. Other methodologies, such as multilocus sequence analysis (MLSA), have been used in such cases, with good results. In this study, three strains (SEMIAs 690T, 6387 and 6428) of the genus Bradyrhizobium, isolated from nitrogen-fixing nodules of Centrosema and Acacia species, without clear taxonomic positions, were studied. These strains differed from genetically closely related species according to the results of MLSA of four housekeeping genes (dnaK, glnII, gyrB and recA) and nucleotide identities of the concatenated genes with those of related species ranged from 87.8 % to 95.7 %, being highest with Bradyrhizobium elkanii. DNA?DNA hybridization (less than 32 % DNA relatedness) and average nucleotide identity values of the whole genomes (less than 90.5 %) indicated that these strains represented a novel species, and phenotypic traits were determined. Our data supported the description of the SEMIA strains as Bradyrhizobium viridifuturi sp. nov., and SEMIA 690T (5CNPSo 991T5C 100aT5BR 1804T5LMG 28866T), isolated from Centrosema pubescens, was chosen as type strain.
653 $aFixação biologica de nitrogenio
700 1 $aDELAMUTA, J. R. M.
700 1 $aRIBEIRO. R. A.
700 1 $aORMEÑO-ORRILLO, E.
700 1 $aROGEL, M. A
700 1 $aMARTINEZ-ROMERO, E.
700 1 $aHUNGRIA, M.
773 $tInternational Journal of Systematic and Evolutionary Microbiology, Reading$gv. 65, n. 12, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 24 | |
| 5. |  | KLEPA, M. S.; HELENE, L. C. F.; O´HARA, G.; HUNGRIA, M. Bradyrhizobium agreste sp. nov., Bradyrhizobium glycinis sp. nov. and Bradyrhizobium diversitatis sp. nov., isolated from a biodiversity hotspot of the genus Glycine in Western Australia. International Journal of Systematic and Evolutionary Microbiology, v. 71, n. 3, 004742, 2021. 13 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | HELENE, L. C. F.; KLEPA, M. S.; O'HARA, G.; HUNGRIA, M. Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia International journal of systematic and evolutionary microbiology, v. 70, n. 8, p. 4623-4636, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | KLEPA, M. S.; HELENE, L. C. F.; O´HARA, G.; HUNGRIA, M. Bradyrhizobium cenepequi sp. nov., Bradyrhizobium semiaridum sp. nov., Bradyrhizobium hereditatis sp. nov. and Bradyrhizobium australafricanum sp. nov., symbionts of different leguminous plants of Western Australia and South Africa and definition of three novel symbiovars. International Journal of Systematic and Evolutionary Microbiology, v. 72, n. 7, 005446, 2022. 18 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | HELENE, L. C. F.; DALL'AGNOL, R. F.; DELAMUTA, J. R. M.; HUNGRIA, M. Mesorhizobium atlanticum sp. nov., a new nitrogen-fixing species from soils of the Brazilian Atlantic Forest biome. International Journal of Systematic and Evolutionary Microbiology, v. 69, n. 6, p. 1800-1806, jun. 2019. p. 1800-1806,| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 14. | | MOURA, F. T.; HELENE, L. C. F.; RIBEIRO, R. A.; NOGUEIRA, M. A.; HUNGRIA, M. The outstanding diversity of rhizobia microsymbionts of common bean (Phaseolus vulgaris L.) in Mato Grosso do Sul, central-western Brazil, revealing new Rhizobium species. Archives of Microbiology, v. 205, 325, 2023. 16 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 16. | | HELENE, L. C. F.; DELAMUTA, J. R. M.; RIBEIRO, R. A.; HUNGRIA, M. Determinação da filogenia de rizóbios por multilocus sequence analysis (mlsa) com identificação e descrição de novas espécies. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 145. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 20. | | BARBOSA, R. L.; HELENE, L. C. F.; RONDINA, A. B. L.; GUIMARÃES, G. S.; HUNGRIA, M.; NOGUEIRA, M. A. Exopolímeros bacterianos como protetores celulares em inoculantes para soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 18., 2023, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2023. (Embrapa Soja. Documentos, 453). Regina Maria Villas Bôas de Campos Leite, Larissa Alexandra Cardoso Moraes, Kelly Catharin, Editoras Técnicas. p. 106-112.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 24 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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