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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
20/02/2013 |
Data da última atualização: |
09/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DORNELES, E. M. S.; SANTANA, J. A.; ANDRADE, G. I.; SANTOS, E. L. S.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. S.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; LAGE, A. P.; HEINEMANN, M. B. |
Afiliação: |
E. M. S. DORNELES, UFMG; J. A. SANTANA, UFMG; G. I. ANDRADE, UFMG; E. L. S. SANTOS, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; R. A. MORA, UFRP; A. S. SANTOS, UFRP; A. MIYOSHI, UFMG; V. AZEVEDO, UFMG; A. M. G. GOUVEIA, UFMG; A. P. LAGE, UFMG; M. B. HEINEMANN, UFMG. |
Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 153, n. 3/4, p. 299-306, 2011 |
DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
Idioma: |
Inglês |
Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R + ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1 + 2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1 + 2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P < 5.01 × 10−6). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R + ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1 + 2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1 + 2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P < ... Mostrar Tudo |
Palavras-Chave: |
Cabras; ERIC-PCR. |
Thesagro: |
Corynebacterium Pseudotuberculosis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02621naa a2200301 a 4500 001 1950191 005 2024-02-09 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI 100 1 $aDORNELES, E. M. S. 245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR.$h[electronic resource] 260 $c2011 520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R + ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1 + 2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1 + 2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P < 5.01 × 10−6). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. 650 $aCorynebacterium Pseudotuberculosis 653 $aCabras 653 $aERIC-PCR 700 1 $aSANTANA, J. A. 700 1 $aANDRADE, G. I. 700 1 $aSANTOS, E. L. S. 700 1 $aGUIMARAES, A. S. 700 1 $aMOTA, R. A. 700 1 $aSANTOS, A. S. 700 1 $aMIYOSHI, A. 700 1 $aAZEVEDO, V. 700 1 $aGOUVEIA, A. M. G. 700 1 $aLAGE, A. P. 700 1 $aHEINEMANN, M. B. 773 $tGenetics and Molecular Research$gv. 153, n. 3/4, p. 299-306, 2011
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Embrapa Gado de Leite (CNPGL) |
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Registros recuperados : 35 | |
1. | | RODRIGUES, R. O.; HERRMANN, G. P.; HEINEMANN, M. B.; LAGE, A. P.; LOPES, L. B.; MOREIRA, E. C. Comparação entre a imunidade induzida em bovinos vacinados com bacterinas polivalentes comerciais e uma monovalente experimental. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 31, n. 1, p. 10-16, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Agrossilvipastoril. |
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2. | | OLIVEIRA, A. P.; HEINEMANN, M. B.; CORTEZ, A.; NASCIMENTO, P. M. P. do; LEITE, R. C.; VIANA, J. H. M. Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows. Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.676-679, maio, 2016. Nota científica.Tipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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3. | | GONÇALVES, M. S.; DORNELES, E. M. S.; HEINEMANN, M. B.; BRITO, M. A. V. P. e; GUIMARÃES, A. S. Genetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Minas Gerais, Brazil. Ciência Rural, Santa Maria, v. 53, n. 3, e20210643, 2023.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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4. | | NASCIMENTO-PENIDO, P. M. P.; PENIDO, A. O.; GALINARI, G. C. F.; HEINEMANN, M. B.; LEITE, R. C. Ocorrência do vírus da artrite encefalite caprina (CAEV) em cabras leiteiras produzidas em sistema intensivo confinado no estado de Minas Gerais. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 577-581, jun. 2017. Título em inglês: Occurrence of caprine arthritis encephalitis virus (CAEV) in dairy goats produced in an intensive confined system in the state of Minas Gerais, Brazil.Biblioteca(s): Embrapa Unidades Centrais. |
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5. | | MINARDI, J. C.; GOUVEIA, A. M. G.; BRAZ, G. F.; ANDRIOLI, A.; PINHEIRO, R. R.; HEINEMANN, M. B. Venereal shedding pattern of caprine lentivirus in semen of infected bucks. In: WORLD BUIATRICS CONGRESS, 27., 2012, Lisboa. Proceedings... Lisboa: APB, 2012. Publicado em: Revista Portuguesa de Buiatria - Abstract Book. Lisboa, 2012. Abstract P:1069, p. 322-323.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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6. | | MUSSI, J. M. S.; GOUVEIA, A. M. G.; CORTEZ, A.; LAGE, A. P.; GUIMARAES, A. S.; HEINEMANN, M. B. Use of serological diagnostic techniques in the control and eradication of caprine arthritis encephalitis: an update. Brazilian Journal of Veterinary Research and Animal Science, v. 52, n. 4, p. 283-297, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Gado de Leite. |
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8. | | BOTONI, L. S.; SCHERER, C. B.; SILVA, R. O.; COURA, F. M.; HEINEMANN, M. B.; PAES-LEME, F. O; COSTA-VAL, A. P. Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1178-1180, dez. 2016.Biblioteca(s): Embrapa Unidades Centrais. |
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9. | | DORNELES, E. M. S.; MORCATTI, F. C.; GUIMARAES, A. S.; LOBATO, Z. I. P.; LAGE, A. P.; GONÇALVES, V. S. P.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra o virus da lingua azul em ovinos do Distrito Federal. Semina, v. 33, n. 4, p. 1521-1524, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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10. | | AIZAWA, J.; SOUZA-FILHO, A. F.; GUIMARÃES, A. S.; VASCONCELOS, C. G. C.; BRITO, M. A. V. P.; SELLERA, F. P.; CORTEZ, A.; HEINEMANN, M. B. Retrospective multicenter study reveals absence of MRSA-associated bovine mastitis in Brazil (1994 to 2016). The Journal of Infection in Developing Countries, v. 13, n. 6, p. 581-583, 2019.Tipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Gado de Leite. |
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12. | | GUIMARAES, A. S.; CARMO, F. B.; HEINEMANN, M. B.; PORTELA, R. W. D.; MEYER, R.; LAGE, A. P.; SEYFFERT, N.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G. High sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of Minas Gerais, Brazil. BMC Veterinary Research, v. 7, article 68, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Gado de Leite. |
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13. | | RAMOS, C. P.; DORNELES, E. M. S.; HAAS, D. J.; VESCHI, J. L. A.; LOUREIRO, D.; PORTELA, R. D.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. Ciência Rural, v. 52, n. 11, e20210328, 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Semiárido. |
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14. | | GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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15. | | MONTEIRO, K.; ALLENDORF, S. D.; VICENTE, A. F.; APPOLINÁRIO, C. M.; PERES, M. G.; CORTEZ, A.; HEINEMANN, M. B.; MEGID, J. Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1181-1185, dez. 2016.Biblioteca(s): Embrapa Unidades Centrais. |
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16. | | AIZAWA, J.; SOUZA FILHO, A. F.; BRITO, M. A. V. P. e; LANGE, C. C.; GUIMARAES, A. S.; SANTOS, R. P.; SOUZA, F. N.; SILVEIRA, A. C. O.; VASCONCELOS, C. G. C.; CORTEZ, A.; HEINEMANN, M. B. Absence of methicillin resistance in staphylococcus aureus isolated from bovine mastitis in southeast Brazil In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14., 2016, Rosário. Resúmenes. Buenos Aires: Asociación Argentina de Microbiología, 2016.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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17. | | CRUZ, J. C. M.; GOUVEIA, A. M. G.; SOUZA, K. C. de; BRAZ, G. F.; TEIXEIRA, B. M.; HEINEMANN, M. B.; LEITE, R. C.; REIS, J. K. P.; PINHEIRO, R. R.; ANDRIOLI, A. Caprine arthritis-encephalitis virus (CAEV) detection in semen of endangered goat breeds by nested polymerase chain reaction. Small Ruminant Research, v. 85, n. 2/3, p. 149-152, 2009.Tipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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18. | | FONSECA JÚNIOR, A. A.; NONAKA, C. K. V.; GUEDES, E. de O.; LOBATO, Z. I. P.; DIAS, A. S.; NASCIMENTO, J. A. F. B. do; KLEIN, C. S.; REIS, J. K. P. dos; HEINEMANN, M. B. Detecção de agentes associados com doenças respiratórias de suínos por PCR em tempo real. Revista Brasileira de Saúde e Produção Animal, v. 16, n. 2, p. 300-307, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Suínos e Aves. |
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19. | | PÉREZ, V. K. C.; MAGOSSI, E. M.; OLIVEIRA, P. F. R. e; CUSTODIO, D. A. C.; CRUZ, H. F. DA; ABREU, J. P. DE; HEINEMANN, M. B.; GUIMARÃES, A. S.; DORNELES, E. M. Detection of biofilm-forming Staphylococcus aureus isolated from bovine mastitis and association with antimicrobial resistance in Brazil. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 24., 2018, Santiago, Chile. Resúmenes... Santiago: ALAM, 2018. p. 201.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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20. | | CARMO, F. B. DO; GUIMARAES, A. S.; PAULETTI, R. B.; LAGE, A. P.; GONÇALVES, V. S. P.; MEYER, R.; PORTELA, R. W. B.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra a linfadenite caseosa em criações comerciais de ovinos no Distrito Federal, Brasil. Arquivos do Instituto Biológico, v. 79, n. 2, p. 293-298, 2012.Tipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 35 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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