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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/08/2021 |
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Data da última atualização: |
19/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. |
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Afiliação: |
VERÓNICA K. C. PÉREZ, Universidade Federal de Lavras, Lavras; DIRCÉIA A. C. CUSTÓDIO, Universidade Federal de Lavras; EDUARDA M. M. SILVA, Universidade Federal de Lavras; JULIA DE OLIVEIRA, Universidade Federal de Lavras; ALESSANDRO DE SA GUIMARAES, CNPGL; MARIA A. V. P. BRITO; ANTÔNIO F. SOUZA-FILHO, Universidade de São Paulo; MARCOS B. HEINEMANN, Universidade de São Paulo; ANDREY P. LAGE, Universidade Federal de Minas Gerais; ELAINE M. S. DORNELES, Universidade Federal de Lavras. |
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Título: |
Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00363-5 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. MenosThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), er... Mostrar Tudo |
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Palavras-Chave: |
Hemolisina; Infecção intramamária; Intramammary infection; Mastite; Staphylococci. |
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Thesagro: |
Biofilme; Bovino; Doença Animal; Estafilococo. |
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Thesaurus Nal: |
Biofilm; Hemolysins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03482naa a2200373 a 4500
001 2133706
005 2021-08-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00363-5$2DOI
100 1 $aPEREZ, V. K. C.
245 $aVirulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.$h[electronic resource]
260 $c2020
520 $aThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin.
650 $aBiofilm
650 $aHemolysins
650 $aBiofilme
650 $aBovino
650 $aDoença Animal
650 $aEstafilococo
653 $aHemolisina
653 $aInfecção intramamária
653 $aIntramammary infection
653 $aMastite
653 $aStaphylococci
700 1 $aCUSTODIO, D. A. C.
700 1 $aSILVA, E. M. M.
700 1 $aOLIVEIRA, J. de
700 1 $aGUIMARÃES, A. S.
700 1 $aBRITO, M. A. V. P.
700 1 $aSOUZA-FILHO, A. F.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
700 1 $aDORNELES, E. M. S.
773 $tBrazilian Journal of Microbiology$gv. 51, p. 2111-2122, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 35 | |
| 21. |  | FONSECA JÚNIOR, A. A.; NONAKA, C. K. V.; GUEDES, E. de O.; LOBATO, Z. I. P.; DIAS, A. S.; NASCIMENTO, J. A. F. B. do; KLEIN, C. S.; REIS, J. K. P. dos; HEINEMANN, M. B. Detecção de agentes associados com doenças respiratórias de suínos por PCR em tempo real. Revista Brasileira de Saúde e Produção Animal, v. 16, n. 2, p. 300-307, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
|    |
| 22. | | PÉREZ, V. K. C.; MAGOSSI, E. M.; OLIVEIRA, P. F. R. e; CUSTODIO, D. A. C.; CRUZ, H. F. DA; ABREU, J. P. DE; HEINEMANN, M. B.; GUIMARÃES, A. S.; DORNELES, E. M. Detection of biofilm-forming Staphylococcus aureus isolated from bovine mastitis and association with antimicrobial resistance in Brazil. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 24., 2018, Santiago, Chile. Resúmenes... Santiago: ALAM, 2018. p. 201.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 23. | | DORNELES, E. M. S.; ANDRADE, G. I.; SANTOS, E. L. DE S.; SANTANA, J. A.; LAGE, A. P.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. DE S.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using eric-PCR. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 24. | | DORNELES, E. M. S.; SANTANA, J. A.; ANDRADE, G. I.; SANTOS, E. L. S.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. S.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; LAGE, A. P.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR. Genetics and Molecular Research, v. 153, n. 3/4, p. 299-306, 2011| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 25. | | CARMO, F. B. DO; GUIMARAES, A. S.; PAULETTI, R. B.; LAGE, A. P.; GONÇALVES, V. S. P.; MEYER, R.; PORTELA, R. W. B.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra a linfadenite caseosa em criações comerciais de ovinos no Distrito Federal, Brasil. Arquivos do Instituto Biológico, v. 79, n. 2, p. 293-298, 2012.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 26. | | CARMO, F. B. do; GUIMARÃES, A. de S.; PAULETTI, R. B.; LAGE, A. P.; GOUVEIA, A. M. G.; FERREIRA, F.; PINHEIRO, R. R.; PORTELA, R. W. D.; AZEVEDO, V. A. de C.; HEINEMANN, M. B. Soroprevalência da linfadenite caseosa em caprinos em propriedades no estado do Ceará In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25.,2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2009. Resumo 2103-1.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 27. | | SALES, M. L.; FONSECA JUNIOR, A. A.; ORZIL, L.; ALENCAR, A. P.; HODON, M. A.; ISSA, M. A.; SOARES FILHO, P. M.; SILVA, M. R.; LAGE, A. P.; HEINEMANN, M. B. Validation of two real-time PCRs targeting the PE-PGRS 20 gene and the region of difference 4 for the characterization of Mycobacterium bovis isolates. Genetics and Molecular Research, v. 13, n. 2, p. 4607-16, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 28. | | PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 29. | | LEAL FILHO, J. M.; BOTTENE, I. F. N.; MONTEIRO, L. A. R. C.; PELLEGRIN, A. O.; GONÇALVES, V. S. P.; FERREIRA, F.; DIAS, R. A.; AMAKU, M.; TELLES, E. O.; GRISI FILHO, J. H. H.; HEINEMANN, M. B.; FERREIRA NETO, J. S. Control of bovine brucellosis from 1998 to 2009 in the state of Mato Grosso do Sul, Brazil. Semina: Ciências Agrárias, Londrina, v. 37, n. 5, suplemento 2, p. 3467-3478, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| 30. | | CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 31. | | HOMEM, V. S. F.; HEINEMANN, M. B.; MORAES, Z. M.; VIANNA, M. C. B.; SILVA, J. C. R.; SAKAMOTO, S. M.; PINHEIRO, S. R.; VEIGA, J. B. da; LAU, H. D.; QUANZ, D.; TOURRAND, J.-F.; FERREIRA, F.; FERREIRA NETO, J. S. Leptospirose bovina em Uruará, PA, município da Amazônia Oriental. Arquivos do Instituto Biológico, v. 67, n. 1, p. 1-10, 2000.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 32. | | DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. Plos One, v. 9, n. 6, e98758, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 33. | | DORNELES, E. M. S.; GUIMARÃES, A. S.; GOUVEIA, A. M. G.; COURA, F. M.; CARMO, F. B. do; PAULETTI, R. B.; AZEVEDO, V.; LILENBAUM, W.; VITOR, V. de A.; PINHEIRO, R. R.; FERREIRA, A. C. R.; DASSO, M. G.; LAGE, A. P.; HEINEMANN, M. B. Seroprevalence of Brucella ovis-epididymitis, smooth-Brucella, leptospirosis, toxoplasmosis, and Maedi-Visna in sheep slaughtered in Minas Gerais State, Brazil. Brazilian Journal of Veterinary Research and Animal Science, v. 57, n. 2, e164278, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 34. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 35. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N. de; LAGE, A. P.; BLAGITZ, M. G.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 35 | |
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