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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
04/08/2023 |
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Data da última atualização: |
04/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, R. S.; COSTA, V. V.; GOMES, G. L. M.; CAMPANA, P. R. V.; PÁDUA, R. M. de; BARBOSA, M.; OKI, Y.; HEIDEN, G.; FERNANDES, G. W.; OLIVEIRA, D. M. de; SOUZA, D. G.; TEIXEIRA, M. M.; BRAGA, F. C. |
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Afiliação: |
ROSÂNGELA SANTOS PEREIRA, Universidade Federal de Minas Gerais; VIVIAN VASCONCELOS COSTA, Universidade Federal de Minas Gerais; GABRIEL LUIZ MENEZES GOMES, Universidade Federal de Minas Gerais; PRISCILLA RODRIGUES VALADARES CAMPANA, Universidade Federal de Minas Gerais; RODRIGO MAIA DE PÁDUA, Universidade Federal de Minas Gerais; MILTON BARBOSA, Universidade Federal de Minas Gerais; YUMI OKI, Universidade Federal de Minas Gerais; GUSTAVO HEIDEN, CPACT; GERALDO WILSON FERNANDES, Universidade Federal de Minas Gerais; DJALMA MENEZES DE OLIVEIRA, Universidade Estadual do Sudoeste da Bahia; DANIELE G. SOUZA, Universidade Federal de Minas Gerais; MAURO MARTINS TEIXEIRA, Universidade Federal de Minas Gerais; FERNÃO CASTRO BRAGA, Universidade Federal de Minas Gerais. |
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Título: |
Anti-Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL-81 and Human Neuroblastoma SH-SY5Y Cells. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Chemistry & Biodiversity, v. 19, n. 4, e202100842, April 2022. |
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DOI: |
https://doi.org/10.1002/cbdv.202100842 |
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Idioma: |
Inglês |
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Conteúdo: |
Zika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV-infected Vero CCL-81 cells were treated with samples at non-cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration-dependent anti-ZIKV effect, with viral loads reduction from 0.4 to 3.8?log units. The 12 active extracts were tested on ZIKV-infected SH-SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5?log), Terminalia phaeocarpa (3.7?log), Maytenus rigida (1.7?log) and Echinodorus grandiflorus (1.7?log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH-SY5Y lineage. M. ilicifolia (IC50=16.8±10.3??g/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8??g/mL, SI=4.8) were the most active extracts. UPLC-ESI-MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti-ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes were identified as the probable bioactive constituents of T. phaeocarpa and M. ilicifolia, respectively. MenosZika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV-infected Vero CCL-81 cells were treated with samples at non-cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration-dependent anti-ZIKV effect, with viral loads reduction from 0.4 to 3.8?log units. The 12 active extracts were tested on ZIKV-infected SH-SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5?log), Terminalia phaeocarpa (3.7?log), Maytenus rigida (1.7?log) and Echinodorus grandiflorus (1.7?log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH-SY5Y lineage. M. ilicifolia (IC50=16.8±10.3??g/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8??g/mL, SI=4.8) were the most active extracts. UPLC-ESI-MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti-ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes w... Mostrar Tudo |
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Thesagro: |
Biodiversidade; Extrato Vegetal; Vírus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155672/1/Artigo-Anti8208Zika-Virus-Activity-of-Plant-Extracts.pdf
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Marc: |
LEADER 02560naa a2200313 a 4500
001 2155672
005 2023-08-04
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/cbdv.202100842$2DOI
100 1 $aPEREIRA, R. S.
245 $aAnti-Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL-81 and Human Neuroblastoma SH-SY5Y Cells.$h[electronic resource]
260 $c2022
520 $aZika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV-infected Vero CCL-81 cells were treated with samples at non-cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration-dependent anti-ZIKV effect, with viral loads reduction from 0.4 to 3.8?log units. The 12 active extracts were tested on ZIKV-infected SH-SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5?log), Terminalia phaeocarpa (3.7?log), Maytenus rigida (1.7?log) and Echinodorus grandiflorus (1.7?log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH-SY5Y lineage. M. ilicifolia (IC50=16.8±10.3??g/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8??g/mL, SI=4.8) were the most active extracts. UPLC-ESI-MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti-ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes were identified as the probable bioactive constituents of T. phaeocarpa and M. ilicifolia, respectively.
650 $aBiodiversidade
650 $aExtrato Vegetal
650 $aVírus
700 1 $aCOSTA, V. V.
700 1 $aGOMES, G. L. M.
700 1 $aCAMPANA, P. R. V.
700 1 $aPÁDUA, R. M. de
700 1 $aBARBOSA, M.
700 1 $aOKI, Y.
700 1 $aHEIDEN, G.
700 1 $aFERNANDES, G. W.
700 1 $aOLIVEIRA, D. M. de
700 1 $aSOUZA, D. G.
700 1 $aTEIXEIRA, M. M.
700 1 $aBRAGA, F. C.
773 $tChemistry & Biodiversity$gv. 19, n. 4, e202100842, April 2022.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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Tipo/Formato |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
29/04/2021 |
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Data da última atualização: |
04/11/2022 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
BERNI, R. F.; CHAVES, F. C. M. |
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Afiliação: |
RODRIGO FASCIN BERNI, CPAA; FRANCISCO CELIO MAIA CHAVES, CPAA. |
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Título: |
Crescimento e marcha de absorção de nutrientes em tomateiro sob cultivo protegido na região de Manaus, AM. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Manaus: Embrapa Amazônia Ocidental, 2021. |
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Páginas: |
21 p. |
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Série: |
(Embrapa Amazônia Ocidental. Boletim de pesquisa e desenvolvimento, 37). |
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ISSN: |
1517-2457 |
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Idioma: |
Português |
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Notas: |
ODS 2 |
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Conteúdo: |
Objetivou-se avaliar o crescimento e a marcha de absorção de nutrientes em tomateiro do tipo Italiano ("saladete"), cultivar BRS Nagai, sob cultivo protegido em vaso preenchido com substrato de fibra de coco e fertirrigado, na região de Manaus, AM. |
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Palavras-Chave: |
Combate a fome; Sustentabilidade. |
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Thesagro: |
Cocos Nucifera; Cultivo Protegido; Fertirrigação; Tomate. |
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Thesaurus NAL: |
Solanum lycopersicum. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222921/1/BolPesq.pdf
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Marc: |
LEADER 01040nam a2200253 a 4500
001 2131559
005 2022-11-04
008 2021 bl uuuu u0uu1 u #d
022 $a1517-2457
100 1 $aBERNI, R. F.
245 $aCrescimento e marcha de absorção de nutrientes em tomateiro sob cultivo protegido na região de Manaus, AM.$h[electronic resource]
260 $aManaus: Embrapa Amazônia Ocidental$c2021
300 $a21 p.
490 $a(Embrapa Amazônia Ocidental. Boletim de pesquisa e desenvolvimento, 37).
500 $aODS 2
520 $aObjetivou-se avaliar o crescimento e a marcha de absorção de nutrientes em tomateiro do tipo Italiano ("saladete"), cultivar BRS Nagai, sob cultivo protegido em vaso preenchido com substrato de fibra de coco e fertirrigado, na região de Manaus, AM.
650 $aSolanum lycopersicum
650 $aCocos Nucifera
650 $aCultivo Protegido
650 $aFertirrigação
650 $aTomate
653 $aCombate a fome
653 $aSustentabilidade
700 1 $aCHAVES, F. C. M.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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