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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
21/03/2023 |
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Data da última atualização: |
01/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOCEMA, M. L.; MORAES, T. DE S.; ATTÍLIO, L. B.; MARQUES, J. P. R.; STIPP, L. C. L.; HARAKAVA, R.; ASTUA, J. de F.; BELASQUE JUNIOR, J.; AZEVEDO, R. A.; GAZIOLA, S. A.; MOURÃO FILHO, F. DE A. A. |
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Afiliação: |
MATHEUS LUÍS DOCEMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; TATIANA DE SOUZA MORAES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JOÃO PAULO RODRIGUES MARQUES, UNIVERSIDADE DE SÃO PAULO; LILIANE CRISTINA LIBORIO STIPP, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; JULIANA DE FREITAS ASTUA, CNPMF; JOSÉ BELASQUE JUNIOR, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; RICARDO ANTUNES AZEVEDO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SALETE APARECIDA GAZIOLA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ. |
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Título: |
Transgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, 2022. |
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DOI: |
https://doi.org/10.1007/s11240-022-02420-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Currently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sieve tube elements of leaf tissues in some transgenic events. Callose accumulation is an important plant defense response to biotic stresses and, in csd1 transgenic lines, it was associated with lower Xcc populations. MenosCurrently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sie... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica; Planta Transgênica. |
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Thesaurus Nal: |
Antimicrobial peptides; Citrus; Superoxide dismutase; Transgenic plants; Xanthomonas citri subsp. citri. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02770naa a2200337 a 4500
001 2152545
005 2023-06-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-022-02420-3$2DOI
100 1 $aDOCEMA, M. L.
245 $aTransgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease.$h[electronic resource]
260 $c2022
520 $aCurrently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sieve tube elements of leaf tissues in some transgenic events. Callose accumulation is an important plant defense response to biotic stresses and, in csd1 transgenic lines, it was associated with lower Xcc populations.
650 $aAntimicrobial peptides
650 $aCitrus
650 $aSuperoxide dismutase
650 $aTransgenic plants
650 $aXanthomonas citri subsp. citri
650 $aFruta Cítrica
650 $aPlanta Transgênica
700 1 $aMORAES, T. DE S.
700 1 $aATTÍLIO, L. B.
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aHARAKAVA, R.
700 1 $aASTUA, J. de F.
700 1 $aBELASQUE JUNIOR, J.
700 1 $aAZEVEDO, R. A.
700 1 $aGAZIOLA, S. A.
700 1 $aMOURÃO FILHO, F. DE A. A.
773 $tPlant Cell, Tissue and Organ Culture, 2022.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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| Registros recuperados : 49 | |
| 41. |  | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; BANGUELA-CASTILLO, E.; TASSI, A. D.; RODRIGUES, M. da C.; KITAJIMA, E. W.; HARAKAVA, R.; ASTUA, J. de F. Unveiling the complete genome sequence of clerodendrum chlorotic spot virus, a putative dichorhavirus infecting ornamental plants. Archives of Virology, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 42. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; PEREIRA, L. R.; BASTIANEL, M.; LAU, D.; CANALE, M. C.; HARAKAVA, R.; NOVELLI, V. M.; KITAJIMA, E. W. N.; ASTUA, J. de F. Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. Plants, v.12, n.6 p.1371. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Trigo. |
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| 43. | | AZEVEDO, F. A. de; MOURÃO FILHO, F. de A. A.; SCHINOR, E. H.; PAOLI, L. G. de; MENDES, B. M. J.; HARAKAVA, R.; GABRIEL, D. W.; LEE, R. F. GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 11, p. 1623-1628, nov. 2006 Título em português: Expressão do gene GUS controlado por promotor de citros em plantas transgênicas de tabaco e laranja 'Valência'.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 44. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; POSTCLAM-BARRO, M.; FONTENELE, RA. S.; HARAKAVA, R.; BASSANEZI, R. B.; MOREIRA, A. S.; KITAJIMA, E. W.; VARSANI, A.; ASTUA, J. de F. Molecular epidemiology of Citrus Leprosis Virus C: a new viral lineage and phylodynamic of the main viral subpopulations in the Americas. Frontiers in Microbiology, V. 12, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 45. | | ZANOTTA, S.; TOFOLI, J. G.; SALAS, F. J. S.; TERÇARIOL, I. M. L.; DOMINGUES, R. J.; FERRARI, J. T.; RIVAS, E. B.; PRADO, S. de S.; SILVA, G. A. C.; MARCIANO, C. S.; HARAKAVA, R. Distribuição dos grupos de compatibilidade A1 e A2 de Phytophthora infestans nas regiões sul e sudeste do Brasil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 49., 2016, Maceió. Anais... Maceió: Sociedade Brasileira de Fitopatologia, 2016. Ref. 586.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 46. | | JESUS, C. C. de; GONZÁLEZ, P. L. R.; TOLEDO, P. de F.; HARAKAVA, R.; TASSIRA, A.; BURILLE, F.; OLIVEIRA, S. S.; SANTOS FILHO, H. P.; KITAJIMA, E. W.; ASTUA, J. de F. First report of passion fruit green spot virus in the state of Mato Grosso, Brazil. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 28.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 12., 2017, Belo Horizonte. Trabalho pôsteres... Brasília: SBV, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 47. | | DOCEMA, M. L.; MORAES, T. DE S.; ATTÍLIO, L. B.; MARQUES, J. P. R.; STIPP, L. C. L.; HARAKAVA, R.; ASTUA, J. de F.; BELASQUE JUNIOR, J.; AZEVEDO, R. A.; GAZIOLA, S. A.; MOURÃO FILHO, F. DE A. A. Transgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease. Plant Cell, Tissue and Organ Culture, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 48. | | GONZÁLEZ, P. L. R.; ALEXANDRE, M. A. V.; POTSCLAM-BARRO, M.; DUARTE, L. M. L.; GONZALEZ, G. L. M.; CHABI-JESUS, C.; RAMOS, A. F.; HARAKAVA, R.; LORENZI, H.; ASTUA, J. de F.; KITAJIMA, E. W. Two Novel Betarhabdovirins Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii. Viruses, v. 16, n. 322, 2024. 17 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 49. | | VIEIRA, L. G. E.; ANDRADE, A. C.; COLOMBO, C. A.; MORAES, A. H. de A.; METHA, A.; OLIVEIRA, A. C. de; LABATE, C. A.; MARINO, C. L.; MONTEIRO-VITORELLO, C. de B.; MONTE, D. C.; GIGLIOTI, E.; KIMURA, E. T.; ROMANO, E.; KURAMAE, E. E.; LEMOS, E. G. M.; ALMEIDA, E. R. P. de; JORGE, E. C.; ALBUQUERQUE, E. V. S.; SILVA, F. R. da; VINECKY, F.; SAWAZAKI, H. E.; DORRY, H. F. A.; CARRER, H.; ABREU, I. N.; BATISTA, J. A. N.; TEIXEIRA, J. B.; KITAJIMA, J. P.; XAVIER, K. G.; LIMA, L. M. de; CAMARGO, L. E. A. de; PEREIRA, L. F. P.; COUTINHO, L. L.; LEMOS, M. V. F.; ROMANO, M. R.; MACHADO, M. A.; COSTA, M. M. do C.; SÁ, M. F. G. de; GOLDMAN, M. H. S.; FERRO, M. I. T.; TINOCO, M. L. P.; OLIVEIRA, M. C.; VAN SLUYS, M-A.; SHIMIZU, M. M.; MALUF, M. P.; EIRA, M. T. S. da; GUERREIRO FILHO, O.; ARRUDA, P.; MAZZAFERA, P.; MARIANI, P. D. S. C.; OLIVEIRA, R. L. B. C. de; HARAKAVA, R.; BALBAO, S. F.; TSAI, S. M.; MAURO, S. M. Z. di; SANTOS, S. N.; SIQUEIRA, W. J.; COSTA, G. G. L.; FORMIGHIERI, E. F.; CARAZZOLLE, M. F.; PEREIRA, G. A. G. Brazilian coffee genome project: an EST-based genomic resource. Brazilian Journal of Plant Physiology, v. 18, n. 1, p. 95-108, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 49 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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